Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins

Microspore embryogenesis is an inducible pathway interesting from basic and applied perspectives. For plant breeding, it is a powerful tool to produce doubled haploids, useful as pure lines. The most efficient way to produce them is through isolated microspore culture. In eggplant, one of the most i...

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Autores: Corral Martínez, Patricia, Seguí-Simarro, Jose M.|||0000-0001-7672-4169
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/63157
Acceso en línea:https://riunet.upv.es/handle/10251/63157
Access Level:acceso abierto
Palabra clave:Androgenesis
Doubled haploid
Haploid
Microspore embryogenesis
Solanum melongena
GENETICA
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spelling Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteinsCorral Martínez, PatriciaSeguí-Simarro, Jose M.|||0000-0001-7672-4169AndrogenesisDoubled haploidHaploidMicrospore embryogenesisSolanum melongenaGENETICAMicrospore embryogenesis is an inducible pathway interesting from basic and applied perspectives. For plant breeding, it is a powerful tool to produce doubled haploids, useful as pure lines. The most efficient way to produce them is through isolated microspore culture. In eggplant, one of the most important vegetable crops, this method is still poorly explored. So far, it is possible to produce doubled haploids, but not directly from embryos, because they are converted into calli early during their development. In this work we evaluated the effect of abscisic acid, epibrassinolide, polyethylene glycol, and arabinogalactans and arabinogalactan proteins, previously described as promoters of embryo induction and development in other species. When added individually to the standard protocol, all of them significantly increased induction of microspore embryogenesis and callus cell proliferation, producing more and larger calli. Particular combinations of them further improved the efficiency of the method. In particular, gum arabic containing arabinogalactans and arabinogalactan proteins allowed embryos to progress beyond the globular stage, constituting a significant improvement in order to achieve the desired direct induction of viable, germinating embryos. We also evaluated the effect of altering the concentration and relative ratio of naphthaleneacetic acid and 6-benzylaminopurine, used in the standard protocol. Significantly better results were obtained by reducing their concentration. Together, our results shed light on the morphogenic and regulatory roles of these substances on microspore embryogenesis, opening ways to further increase the efficiency of production of androgenic doubled haploids through microspore culture in eggplant.We acknowledge the staff of the COMAV greenhouses for their valuable help. Thanks are also due to Dr. Rosa Peiro for her valuable statistical advice. This work was supported by the following grants to JMSS: AGL2010-17895 from Spanish MICINN, ACOMP/2012/168 from Generalitat Valenciana, and PAID-05-11 1909 from Universitat Politecnica de Valencia.Springer Verlag (Germany)Departamento de BiotecnologíaInstituto Universitario de Conservación y Mejora de la Agrodiversidad ValencianaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalMinisterio de Ciencia e InnovaciónGeneralitat ValencianaUniversitat Politècnica de ValènciaRepositorio Institucional de la Universitat Politècnica de València Riunet20142014-02-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/63157reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 AGL2010-17895 GENERACION EFICIENTE DE DOBLE HAPLOIDES EN BERENJENA Y PIMIENTO MEDIANTE CULTIVO IN VITRO DE MICROSPORAS AISLADAS. ANALISIS CELULAR Y MOLECULAR DEL DESARROLLO ANDROGENICOGeneralitat Valenciana https://doi.org/10.13039/501100003359 ACOMP%2F2012%2F168Universitat Politècnica de València https://doi.org/10.13039/501100004233 PAID-05-11 1909open accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/631572026-06-13T07:49:27Z
dc.title.none.fl_str_mv Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins
title Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins
spellingShingle Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins
Corral Martínez, Patricia
Androgenesis
Doubled haploid
Haploid
Microspore embryogenesis
Solanum melongena
GENETICA
title_short Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins
title_full Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins
title_fullStr Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins
title_full_unstemmed Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins
title_sort Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins
dc.creator.none.fl_str_mv Corral Martínez, Patricia
Seguí-Simarro, Jose M.|||0000-0001-7672-4169
author Corral Martínez, Patricia
author_facet Corral Martínez, Patricia
Seguí-Simarro, Jose M.|||0000-0001-7672-4169
author_role author
author2 Seguí-Simarro, Jose M.|||0000-0001-7672-4169
author2_role author
dc.contributor.none.fl_str_mv Departamento de Biotecnología
Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Ministerio de Ciencia e Innovación
Generalitat Valenciana
Universitat Politècnica de València
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Androgenesis
Doubled haploid
Haploid
Microspore embryogenesis
Solanum melongena
GENETICA
topic Androgenesis
Doubled haploid
Haploid
Microspore embryogenesis
Solanum melongena
GENETICA
description Microspore embryogenesis is an inducible pathway interesting from basic and applied perspectives. For plant breeding, it is a powerful tool to produce doubled haploids, useful as pure lines. The most efficient way to produce them is through isolated microspore culture. In eggplant, one of the most important vegetable crops, this method is still poorly explored. So far, it is possible to produce doubled haploids, but not directly from embryos, because they are converted into calli early during their development. In this work we evaluated the effect of abscisic acid, epibrassinolide, polyethylene glycol, and arabinogalactans and arabinogalactan proteins, previously described as promoters of embryo induction and development in other species. When added individually to the standard protocol, all of them significantly increased induction of microspore embryogenesis and callus cell proliferation, producing more and larger calli. Particular combinations of them further improved the efficiency of the method. In particular, gum arabic containing arabinogalactans and arabinogalactan proteins allowed embryos to progress beyond the globular stage, constituting a significant improvement in order to achieve the desired direct induction of viable, germinating embryos. We also evaluated the effect of altering the concentration and relative ratio of naphthaleneacetic acid and 6-benzylaminopurine, used in the standard protocol. Significantly better results were obtained by reducing their concentration. Together, our results shed light on the morphogenic and regulatory roles of these substances on microspore embryogenesis, opening ways to further increase the efficiency of production of androgenic doubled haploids through microspore culture in eggplant.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-02-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/63157
url https://riunet.upv.es/handle/10251/63157
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 AGL2010-17895 GENERACION EFICIENTE DE DOBLE HAPLOIDES EN BERENJENA Y PIMIENTO MEDIANTE CULTIVO IN VITRO DE MICROSPORAS AISLADAS. ANALISIS CELULAR Y MOLECULAR DEL DESARROLLO ANDROGENICO
Generalitat Valenciana https://doi.org/10.13039/501100003359 ACOMP%2F2012%2F168
Universitat Politècnica de València https://doi.org/10.13039/501100004233 PAID-05-11 1909
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Verlag (Germany)
publisher.none.fl_str_mv Springer Verlag (Germany)
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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