Vasoactive intestinal peptide exerts an osteoinductive effect in human mesenchymal stem cells

Several neuropeptides present in bone tissues, produced by nerve fibers and bone cells, have been reported to play a role in regulating the fine-tuning of osteoblast and osteoclast functions to maintain bone homeostasis. This study aims to characterize the influence of the neuropeptide vasoactive in...

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Detalles Bibliográficos
Autores: Castro Vázquez, David, Arribas Castaño, Paula, García López, Iván, Gutiérrez Cañas, Irene, Pérez García, Selene, Lamana Domínguez, Amalia, Villanueva Romero, Raúl, Cabrera Martín, Alicia, Tecza , Karolina, Martínez Mora, María Del Carmen, Juarranz Moratilla, Yasmina, Pérez Gomáriz, Rosa María, Carrión Caballo, Mar
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/104281
Acceso en línea:https://hdl.handle.net/20.500.14352/104281
Access Level:acceso abierto
Palabra clave:577.175.82
576.31
616.72-022.77
VIP
Actin cytoskeleton
Bone metabolism
Human mesenchymal stem cells
Osteogenesis
Biología celular (Biología)
Bioquímica (Biología)
Reumatología
2407.01 Cultivo Celular
2407.03 Morfología Celular
2407 Biología Celular
3205.09 Reumatología
Descripción
Sumario:Several neuropeptides present in bone tissues, produced by nerve fibers and bone cells, have been reported to play a role in regulating the fine-tuning of osteoblast and osteoclast functions to maintain bone homeostasis. This study aims to characterize the influence of the neuropeptide vasoactive intestinal peptide (VIP) on the differentiation process of human mesenchymal stem cells (MSCs) into osteoblasts and on their anabolic function. We describe the mRNA and protein expression profile of VIP and its receptors in MSCs as they differentiate into osteoblasts, suggesting the presence of an autocrine signaling pathway in these cells. Our findings reveal that VIP enhances the expression of early osteoblast markers in MSCs under osteogenic differentiation and favors both bone matrix formation and proper cytoskeletal reorganization. Finally, our data suggest that VIP could be exerting a direct modulatory role on the osteoblast to osteoclast signaling by downregulating the receptor activator of nuclear factor-κB ligand/osteoprotegerin ratio. These results highlight the potential of VIP as an osteoinductive differentiation factor, emerging as a key molecule in the maintenance of human bone homeostasis.

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