Proteomic analysis of synovial fibroblasts and articular chondrocytes co-cultures reveals valuable VIP-modulated inflammatory and degradative proteins in osteoarthritis

Osteoarthritis (OA) is the most common musculoskeletal disorder causing a great disability and a reduction in the quality of life. In OA, articular chondrocytes (AC) and synovial fibroblasts (SF) release innate-derived immune mediators that initiate and perpetuate inflammation, inducing cartilage ex...

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Detalles Bibliográficos
Autores: Pérez García, Selene, Calamia, Valentina, Hermida-Gómez, Tamara, Gutiérrez Cañas, Irene, Carrión Caballo, Mar, Villanueva Romero, Raúl, Castro Vázquez, David, Martínez Mora, María Del Carmen, Juarranz Moratilla, Yasmina, Blanco, Francisco J., Pérez Gomáriz, Rosa María
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/8694
Acceso en línea:https://hdl.handle.net/20.500.14352/8694
Access Level:acceso abierto
Palabra clave:577.175.82
616.72-022.77
Osteoarthritis
Synovial fibroblasts
Chondrocytes
VIP
CHI3L1
PTX3
Complement system
Decorin
Cathepsin B
MMP2
Bioquímica (Medicina)
Inmunología
Reumatología
2412 Inmunología
3205.09 Reumatología
Descripción
Sumario:Osteoarthritis (OA) is the most common musculoskeletal disorder causing a great disability and a reduction in the quality of life. In OA, articular chondrocytes (AC) and synovial fibroblasts (SF) release innate-derived immune mediators that initiate and perpetuate inflammation, inducing cartilage extracellular matrix (ECM) degradation. Given the lack of therapies for the treatment of OA, in this study, we explore biomarkers that enable the development of new therapeutical approaches. We analyze the set of secreted proteins in AC and SF co-cultures by stable isotope labeling with amino acids (SILAC). We describe, for the first time, 115 proteins detected in SF-AC co-cultures stimulated by fibronectin fragments (Fn-fs). We also study the role of the vasoactive intestinal peptide (VIP) in this secretome, providing new proteins involved in the main events of OA, confirmed by ELISA and multiplex analyses. VIP decreases proteins involved in the inflammatory process (CHI3L1, PTX3), complement activation (C1r, C3), and cartilage ECM degradation (DCN, CTSB and MMP2), key events in the initiation and progression of OA. Our results support the anti-inflammatory and anti-catabolic properties of VIP in rheumatic diseases and provide potential new targets for OA treatment.

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