Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopy

We present what is, to our knowledge, the first experimental demonstration of dielectric constant measurement and quantification of supported lipid bilayers in electrolyte solutions with nanoscale spatial resolution. The dielectric constant was quantitatively reconstructed with finite element calcul...

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Authors: Gramse, Georg, Dols-Pérez, Aurora, Edwards, M. A., Fumagalli, Laura, 1959-, Gomila Lluch, Gabriel
Format: article
Status:Published version
Publication Date:2013
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/98162
Online Access:https://hdl.handle.net/2445/98162
Access Level:Open access
Keyword:Nanotecnologia
Bioelectrònica
Microscopis
Nanotechnology
Bioelectronics
Microscopes
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spelling Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopyGramse, GeorgDols-Pérez, AuroraEdwards, M. A.Fumagalli, Laura, 1959-Gomila Lluch, GabrielNanotecnologiaBioelectrònicaMicroscopisNanotechnologyBioelectronicsMicroscopesWe present what is, to our knowledge, the first experimental demonstration of dielectric constant measurement and quantification of supported lipid bilayers in electrolyte solutions with nanoscale spatial resolution. The dielectric constant was quantitatively reconstructed with finite element calculations by combining thickness information and local polarization forces which were measured using an electrostatic force microscope adapted to work in a liquid environment. Measurements of submicrometric dipalmitoylphosphatidylcholine lipid bilayer patches gave dielectric constants of εr ∼ 3, which are higher than the values typically reported for the hydrophobic part of lipid membranes (εr ∼ 2) and suggest a large contribution of the polar headgroup region to the dielectric response of the lipid bilayer. This work opens apparently new possibilities in the study of biomembrane electrostatics and other bioelectric phenomena.Biophysical Society2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/98162Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1016/j.bpj.2013.02.011Biophysical Journal, 2013, vol. 104, num. 6, p. 1257-1262http://dx.doi.org/10.1016/j.bpj.2013.02.011info:eu-repo/grantAgreement/EC/FP7/info:eu-repo/grantAgreement/EC/FP7/228685(c) Biophysical Society, 2013info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/981622026-05-27T06:46:51Z
dc.title.none.fl_str_mv Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopy
title Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopy
spellingShingle Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopy
Gramse, Georg
Nanotecnologia
Bioelectrònica
Microscopis
Nanotechnology
Bioelectronics
Microscopes
title_short Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopy
title_full Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopy
title_fullStr Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopy
title_full_unstemmed Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopy
title_sort Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopy
dc.creator.none.fl_str_mv Gramse, Georg
Dols-Pérez, Aurora
Edwards, M. A.
Fumagalli, Laura, 1959-
Gomila Lluch, Gabriel
author Gramse, Georg
author_facet Gramse, Georg
Dols-Pérez, Aurora
Edwards, M. A.
Fumagalli, Laura, 1959-
Gomila Lluch, Gabriel
author_role author
author2 Dols-Pérez, Aurora
Edwards, M. A.
Fumagalli, Laura, 1959-
Gomila Lluch, Gabriel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Nanotecnologia
Bioelectrònica
Microscopis
Nanotechnology
Bioelectronics
Microscopes
topic Nanotecnologia
Bioelectrònica
Microscopis
Nanotechnology
Bioelectronics
Microscopes
description We present what is, to our knowledge, the first experimental demonstration of dielectric constant measurement and quantification of supported lipid bilayers in electrolyte solutions with nanoscale spatial resolution. The dielectric constant was quantitatively reconstructed with finite element calculations by combining thickness information and local polarization forces which were measured using an electrostatic force microscope adapted to work in a liquid environment. Measurements of submicrometric dipalmitoylphosphatidylcholine lipid bilayer patches gave dielectric constants of εr ∼ 3, which are higher than the values typically reported for the hydrophobic part of lipid membranes (εr ∼ 2) and suggest a large contribution of the polar headgroup region to the dielectric response of the lipid bilayer. This work opens apparently new possibilities in the study of biomembrane electrostatics and other bioelectric phenomena.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/98162
url https://hdl.handle.net/2445/98162
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1016/j.bpj.2013.02.011
Biophysical Journal, 2013, vol. 104, num. 6, p. 1257-1262
http://dx.doi.org/10.1016/j.bpj.2013.02.011
info:eu-repo/grantAgreement/EC/FP7/
info:eu-repo/grantAgreement/EC/FP7/228685
dc.rights.none.fl_str_mv (c) Biophysical Society, 2013
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Biophysical Society, 2013
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Biophysical Society
publisher.none.fl_str_mv Biophysical Society
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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