Concordance between circulating tumor cells and clinical status during follow-up in anaplastic lymphoma kinase (ALK) non-small-cell lung cancer patients

Background: The identification of anaplastic lymphoma kinase (ALK) rearrangements is found in approximately 5% of non-small-cell lung cancers (NSCLCs). However, the development of liquid biopsies as a diagnostic tool is less developed in these cases. This study investigates the use of CTCs during tr...

Descripción completa

Detalles Bibliográficos
Autores: Provencio Pulla, Mariano, Pérez-Callejo, David, Torrente, María, Martín, Paloma, Calvo de Juan, Virginia, Gutiérrez, Lourdes, Franco, Fernando, Coronado, María José, Cruz-Bermúdez, Juan Luis, Martín Ruiz-Valdepeñas, Asunción, Cruz-Bermúdez, Alberto, Sánchez-Beato, Margarita, Romero, Atocha, García-Grande, Aránzazu
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/681151
Acceso en línea:http://hdl.handle.net/10486/681151
https://dx.doi.org/10.18632/oncotarget.19722
Access Level:acceso abierto
Palabra clave:Liquid biopsy
Non-small-cell lung cancer
Circulating tumor cells
ALK-rearrangement
ALK inhibitors
Medicina
Descripción
Sumario:Background: The identification of anaplastic lymphoma kinase (ALK) rearrangements is found in approximately 5% of non-small-cell lung cancers (NSCLCs). However, the development of liquid biopsies as a diagnostic tool is less developed in these cases. This study investigates the use of CTCs during treatment, together with an extended follow-up to correlate with clinical evolution. Patients and Methods: A total of 13 patients out of a cohort of 212 patients with lung adenocarcinoma, presented ALK rearrangements (6%) confirmed by tumor biopsy. A total of 60 serial blood samples were collected from these patients who were prospectively enrolled in the study. Results: All patients had a positive CTC count at baseline (mean = 3). The median follow-up was 9 months (range 1-17 months). Three patients underwent surgery and their CTC counts decreased after the procedure but still remained detectable. After radiotherapy, 3 cases showed an average decrease of 5 CTCs. A total of 6 patients were treated with ALK inhibitors and a partial response was observed in 3 of them, who also presented decreased CTC counts. The other 3 patients presented primary resistance, and their CTC counts were higher than those obtained prior to progression. Conclusion: We believe that the use of CTCs for dynamic monitoring of NSCLC with ALK rearrangement and to detect disease persistence or recurrence may be a reliable technique. CTC counts may also have potential use to monitor the efficacy of ALK inhibitors, facilitating detection of resistance to treatment