Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria

This study was conducted to determine the species distribution, antimicrobial resistance pheno- and genotypes and virulence traits of mannitol-positive methicillin-resistant staphylococci (MRS) isolated from pigs in Nsukka agricultural zone, Nigeria. Twenty mannitol-positive methicillin-resistant co...

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Autores: Ugwu, C.C. [0000-0003-1828-1448], Gomez-Sanz, E. [0000-0001-7666-3642], Agbo, I.C., Torres, C. [0000-0003-3709-1690], Chah, K.F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Universidad de La Rioja (UR)
Repositorio:RIUR. Repositorio Institucional de la Universidad de La Rioja
OAI Identifier:oai:portal.dialnet.es:doc/5bbc6814b750603269e80217
Acceso en línea:https://investigacion.unirioja.es/documentos/5bbc6814b750603269e80217
Access Level:acceso abierto
Palabra clave:Mannitol-fermenting
Methicillin-resistant
Pigs
Quinolone resistance
Staphylococci
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spelling Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in NigeriaUgwu, C.C. [0000-0003-1828-1448]Gomez-Sanz, E. [0000-0001-7666-3642]Agbo, I.C.Torres, C. [0000-0003-3709-1690]Chah, K.F.Mannitol-fermentingMethicillin-resistantPigsQuinolone resistanceStaphylococciThis study was conducted to determine the species distribution, antimicrobial resistance pheno- and genotypes and virulence traits of mannitol-positive methicillin-resistant staphylococci (MRS) isolated from pigs in Nsukka agricultural zone, Nigeria. Twenty mannitol-positive methicillin-resistant coagulase-negative staphylococcal (MRCoNS) strains harboring the mecA gene were detected among the 64 Staphylococcus isolates from 291 pigs. A total of 4 species were identified among the MRCoNS isolates, namely, Staphylococcus sciuri (10 strains), Staphylococcus lentus (6 strains), Staphylococcus cohnii (3 strains) and Staphylococcus haemolyticus (one strain). All MRCoNS isolates were multidrug-resistant. In addition to β-lactams, the strains were resistant to fusidic acid (85%), tetracycline (75%), streptomycin (65%), ciprofloxacin (65%), and trimethoprim/sulpha-methoxazole (60%). In addition to the mecA and blaZ genes, other antimicrobial resistance genes detected were tet(K), tet(M), tet(L), erm(B), erm(C), aacA-aphD, aphA3, str, dfrK, dfrG, cat<inf>pC221</inf>, and cat<inf>pC223</inf>. Thirteen isolates were found to be ciprofloxacin-resistant, and all harbored a Ser84Leu mutation within the QRDR of the GyrA protein, with 3 isolates showing 2 extra substitutions, Ser98Ile and Arg100Lys (one strain) and Glu88Asp and Asp96Thr (2 strains). A phylogenetic tree of the QRDR nucleotide sequences in the gyrA gene revealed a high nucleotide diversity, with several major clusters not associated with the bacterial species. Our study highlights the possibility of transfer of mecA and other antimicrobial resistance genes from MRCoNS to pathogenic bacteria, which is a serious public health and veterinary concern. © 2015, Sociedade Brasileira de Microbiologia.2015info:eu-repo/semantics/articleSubtype: Articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://investigacion.unirioja.es/documentos/5bbc6814b750603269e80217reponame:RIUR. Repositorio Institucional de la Universidad de La Riojainstname:Universidad de La Rioja (UR)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1590/S1517-838246320140644info:eu-repo/semantics/altIdentifier/wos/WOS:000360074700034info:eu-repo/semantics/altIdentifier/pissn/1517-8382Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria, 2015, vol. 46, núm. 3, pág. 885-892info:eu-repo/semantics/openAccessoai:portal.dialnet.es:doc/5bbc6814b750603269e802172026-06-14T12:47:17Z
dc.title.none.fl_str_mv Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria
title Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria
spellingShingle Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria
Ugwu, C.C. [0000-0003-1828-1448]
Mannitol-fermenting
Methicillin-resistant
Pigs
Quinolone resistance
Staphylococci
title_short Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria
title_full Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria
title_fullStr Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria
title_full_unstemmed Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria
title_sort Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria
dc.creator.none.fl_str_mv Ugwu, C.C. [0000-0003-1828-1448]
Gomez-Sanz, E. [0000-0001-7666-3642]
Agbo, I.C.
Torres, C. [0000-0003-3709-1690]
Chah, K.F.
author Ugwu, C.C. [0000-0003-1828-1448]
author_facet Ugwu, C.C. [0000-0003-1828-1448]
Gomez-Sanz, E. [0000-0001-7666-3642]
Agbo, I.C.
Torres, C. [0000-0003-3709-1690]
Chah, K.F.
author_role author
author2 Gomez-Sanz, E. [0000-0001-7666-3642]
Agbo, I.C.
Torres, C. [0000-0003-3709-1690]
Chah, K.F.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Mannitol-fermenting
Methicillin-resistant
Pigs
Quinolone resistance
Staphylococci
topic Mannitol-fermenting
Methicillin-resistant
Pigs
Quinolone resistance
Staphylococci
description This study was conducted to determine the species distribution, antimicrobial resistance pheno- and genotypes and virulence traits of mannitol-positive methicillin-resistant staphylococci (MRS) isolated from pigs in Nsukka agricultural zone, Nigeria. Twenty mannitol-positive methicillin-resistant coagulase-negative staphylococcal (MRCoNS) strains harboring the mecA gene were detected among the 64 Staphylococcus isolates from 291 pigs. A total of 4 species were identified among the MRCoNS isolates, namely, Staphylococcus sciuri (10 strains), Staphylococcus lentus (6 strains), Staphylococcus cohnii (3 strains) and Staphylococcus haemolyticus (one strain). All MRCoNS isolates were multidrug-resistant. In addition to β-lactams, the strains were resistant to fusidic acid (85%), tetracycline (75%), streptomycin (65%), ciprofloxacin (65%), and trimethoprim/sulpha-methoxazole (60%). In addition to the mecA and blaZ genes, other antimicrobial resistance genes detected were tet(K), tet(M), tet(L), erm(B), erm(C), aacA-aphD, aphA3, str, dfrK, dfrG, cat<inf>pC221</inf>, and cat<inf>pC223</inf>. Thirteen isolates were found to be ciprofloxacin-resistant, and all harbored a Ser84Leu mutation within the QRDR of the GyrA protein, with 3 isolates showing 2 extra substitutions, Ser98Ile and Arg100Lys (one strain) and Glu88Asp and Asp96Thr (2 strains). A phylogenetic tree of the QRDR nucleotide sequences in the gyrA gene revealed a high nucleotide diversity, with several major clusters not associated with the bacterial species. Our study highlights the possibility of transfer of mecA and other antimicrobial resistance genes from MRCoNS to pathogenic bacteria, which is a serious public health and veterinary concern. © 2015, Sociedade Brasileira de Microbiologia.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
Subtype: Article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://investigacion.unirioja.es/documentos/5bbc6814b750603269e80217
url https://investigacion.unirioja.es/documentos/5bbc6814b750603269e80217
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/S1517-838246320140644
info:eu-repo/semantics/altIdentifier/wos/WOS:000360074700034
info:eu-repo/semantics/altIdentifier/pissn/1517-8382
Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria, 2015, vol. 46, núm. 3, pág. 885-892
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUR. Repositorio Institucional de la Universidad de La Rioja
instname:Universidad de La Rioja (UR)
instname_str Universidad de La Rioja (UR)
reponame_str RIUR. Repositorio Institucional de la Universidad de La Rioja
collection RIUR. Repositorio Institucional de la Universidad de La Rioja
repository.name.fl_str_mv
repository.mail.fl_str_mv
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