Methicillin-resistant coagulase-negative staphylococci from healthy dogs in Nsukka, Nigeria

The occurrence, resistance phenotype and molecular mechanisms of resistance of methicillinresistant staphylococci from groin swabs of 109 clinically healthy dogs in Nsukka, Nigeria were investigated. The groin swab samples were cultured on mannitol salt agar supplemented with 10 μg of cloxacillin. S...

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Detalles Bibliográficos
Autores: Chah, K.F., Gómez-Sanz, E. [0000-0001-7666-3642], Nwanta, J.A., Asadu, B., Agbo, I.C., Lozano, C. [0000-0002-0048-9315], Zarazaga, M. [0000-0002-8703-5489], Torres, C. [0000-0003-3709-1690]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universidad de La Rioja (UR)
Repositorio:RIUR. Repositorio Institucional de la Universidad de La Rioja
OAI Identifier:oai:portal.dialnet.es:doc/5bbc696cb750603269e81aea
Acceso en línea:https://investigacion.unirioja.es/documentos/5bbc696cb750603269e81aea
Access Level:acceso abierto
Palabra clave:Dogs
Methicillin-resistant
Resistant genes
S. sciuri
Descripción
Sumario:The occurrence, resistance phenotype and molecular mechanisms of resistance of methicillinresistant staphylococci from groin swabs of 109 clinically healthy dogs in Nsukka, Nigeria were investigated. The groin swab samples were cultured on mannitol salt agar supplemented with 10 μg of cloxacillin. Sixteen methicillin-resistant coagulase negative staphylococci (MRCoNS), all harbouring the mecA gene were isolated from 14 (12.8%) of the 109 dogs studied. The MRCoNS isolated were: S. sciuri subspecies rodentium, S. lentus, S. haemolyticus, and S. simulans with S. sciuri subspecies rodentium (62.5%) being the predominant species. Thirteen (81.3%) of the MRCoNS were resistant to tetracycline while 12 (75%) and 10 (62.5%) were resistant to kanamycin and trimthoprim- sulphamethoxazole respectively. None of the isolates was resistant to fusidic acid, linezolid and vancomycin. Thirteen (81.3%) of the MRCoNS were multi-drug resistance (MDR). Other antimicrobial genes detected were: blaZ, tet(K), tet(M), tet(L), erm(B), lnu(A), aacA-aphD, aphA3, str, dfr(G), catpC221,and catpC223. Methicillin-resistant staphylococci are common colonizers of healthy dogs in Nigeria with a major species detected being S. sciuri subsp. rodentium. © 2014, Sociedade Brasileira de Microbiologia.