In Vitro anti-inflammatory activity of iridoids and triterpenoid compounds isolated from Phillyrea latifolia L.
Two iridoids, oleuropeoside and ligustroside, and two triterpenoid compounds, oleanolic acid and ursolic acid, ha re been isolated from the leaves of Phillyrea latifolia L. (Oleaceae). These compounds were tested for interactions with the cyclooxygenase (COX) and 5-lipoxygenase (5-LOX) pathways of a...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2000 |
| País: | España |
| Institución: | Universidad de Alcalá (UAH) |
| Repositorio: | e_Buah Biblioteca Digital Universidad de Alcalá |
| Idioma: | inglés |
| OAI Identifier: | oai:ebuah.uah.es:10017/64382 |
| Acceso en línea: | http://hdl.handle.net/10017/64382 https://dx.doi.org/10.1248/bpb.23.1307 |
| Access Level: | acceso abierto |
| Palabra clave: | Phillyrea latifolia Iridoid Triterpenoid PGE(2) LTC4 TXB2 Farmacia Pharmacy |
| Sumario: | Two iridoids, oleuropeoside and ligustroside, and two triterpenoid compounds, oleanolic acid and ursolic acid, ha re been isolated from the leaves of Phillyrea latifolia L. (Oleaceae). These compounds were tested for interactions with the cyclooxygenase (COX) and 5-lipoxygenase (5-LOX) pathways of arachidonate metabolism in calcium ionophore-stimulated mouse peritoneal macrophages and human platelets, and for their effect on cell viability Structure-activity relationships obtained for in vitro screening results were discussed, These compounds ale capable of exerting inhibitory actions on enzymes of the arachidonate cascade,,ill compounds assayed showed a significant effect on prostaglandin E-2 (PGE(2))-release, with inhibition percentages similar to the reference drug indomethacin (IC50 = 0.95 muM). The IC50 values of the active compounds ate: oleuropeoside 47 muM, ligustroside 38.53 muM, oleanolic acid 23.51 muM and ursolic acid 60.91 muM;. In the leukotriene C-4 (LTC4)-assay, only oleanolic acid showed a significant effect (IC50 = 16.79 muM). We also investigated the action of compounds on thromborane B-2 (TXB2)-release induced by calcium ionophore in human platelets. Of all the tested compounds, only. ligustroside (IC50 = 122.63 muM) and ursolic acid (IC50 = 50.21 muM) showed a significant effect, although with less potency than the reference drug ibuprofen (IC50 = 1.27 muM). Thus, our compounds possess an array of potentially beneficial anti-inflammatory properties which may, alongside other constituents, contribute to the claimed therapeutic properties of the plant from which they are derived. |
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