Genotoxicity of aflatoxin B1 and ochratoxin A after simultaneous application of the in vivo micronucleus and comet assay

Aflatoxin B1 (AFB1) and Ochratoxin A (OTA) are genotoxic mycotoxins that can contaminate a variety of foodstuffs, the liver and the kidney being their target organ, respectively. The micronucleus (MN) assay (bone marrow) and the comet assay (liver and kidney) were performed simultaneously in F344 ra...

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Detalles Bibliográficos
Autores: Corcuera, L.A. (Laura Ana)|||/items/43716f68-33a0-47aa-8d48-1df70c45ac0b, Vettorazzi, A. (Ariane)|||/items/b1ef6cda-f150-427f-b212-9f29c2c4f557, Arbillaga, L. (Leire)|||/items/5d121cf6-ae3a-49f1-853d-2bcd4588ff39, Perez-Hernandez, N. (Noemí)|||/items/173430e1-b0da-4049-a172-c2ada1d26a56, Gil-Royo, A.G. (Ana Gloria)|||/items/f54744d1-e54a-42e1-b5d0-cbabefd3fc53, Azqueta, A. (Amaya)|||/items/f04b3c23-3ff4-41c8-b5e1-4d372fbc4893, Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f, García-Jalón, J.A. (José Antonio)|||/items/aa664ae0-a811-4725-8aa7-a0ae54c27815, Lopez-de-Cerain, A. (Adela)|||/items/8d95b72a-6816-4612-884b-5cf5c6c172f1
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/37258
Acceso en línea:https://hdl.handle.net/10171/37258
Access Level:acceso abierto
Palabra clave:Aflatoxin B1
Ochratoxin A
Mycotoxins
Micronucleus
Comet assay
Combined exposure
Descripción
Sumario:Aflatoxin B1 (AFB1) and Ochratoxin A (OTA) are genotoxic mycotoxins that can contaminate a variety of foodstuffs, the liver and the kidney being their target organ, respectively. The micronucleus (MN) assay (bone marrow) and the comet assay (liver and kidney) were performed simultaneously in F344 rats, treated with AFB1 (0.25 mg/kg b.w.), OTA (0.5 mg/kg b.w.) or both mycotoxins. After AFB1 treatment, histopathology and biochemistry analysis showed liver necrosis, focal inflammation and an increase in Alanine Aminotransferase and Aspartate Aminotransferase. OTA alone did not cause any alteration. The acute hepatotoxic effects caused by AFB1 were less pronounced in animals treated with both mycotoxins. With regard to the MN assay, after 24h, positive results were obtained for AFB1 and negative results were obtained for OTA, although both toxins caused bone marrow toxicity. In the combined treatment, OTA reduced the toxicity and the number of MN produced by AFB1. In the comet assay, after 3h, positive results were obtained for AFB1 in the liver and for OTA in the kidney. The combined treatment reduced DNA damage in the liver and had no influence in the kidney. Altogether, these results may be indicative of an antagonistic relationship regarding the genotoxicity of both mycotoxins.

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