Characterization, stability, and in vivo effects in Caenorhabditis elegans of microencapsulated protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts

This study aimed to microencapsulate protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts produced by Alcalase (HA) and Protamex (HP) by spray drying, using maltodextrin as wall material. The physicochemical characteristics, and in vitro antioxidant and Angiotensin...

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Autores: Oliveira Lima, Karina, Alemán, Ailén, López Caballero, M. Elvira, Gómez Guillén, M. C., Montero García, Pilar, Prentice, Carlos, Taipe Huisa, Andy Joel, Monserrat, José Maria
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/251701
Acesso em linha:http://hdl.handle.net/10261/251701
Access Level:acceso abierto
Palavra-chave:Fish protein hydrolysates
Spray drying
Stability
C. elegans
Physiological effects
Oxidative stress
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spelling Characterization, stability, and in vivo effects in Caenorhabditis elegans of microencapsulated protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproductsOliveira Lima, KarinaAlemán, AilénLópez Caballero, M. ElviraGómez Guillén, M. C.Montero García, PilarPrentice, CarlosTaipe Huisa, Andy JoelMonserrat, José MariaFish protein hydrolysatesSpray dryingStabilityC. elegansPhysiological effectsOxidative stressThis study aimed to microencapsulate protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts produced by Alcalase (HA) and Protamex (HP) by spray drying, using maltodextrin as wall material. The physicochemical characteristics, and in vitro antioxidant and Angiotensin-I converting enzyme-inhibitory activities were evaluated during storage. Both microencapsulated hydrolysates showed spherical shape (~3.6 µm particle diameter), low water activity (<0.155) during storage and reduced hygroscopicity (~30%) compared to the free hydrolysate. Infrared spectroscopy evidenced the maltodextrin-hydrolysate interaction. Based on the in vitro results, nematoid C. elegans in L1 larval stage were treated with free and microencapsulated HP, which demonstrated a protective effect on nematoid exposed to oxidative stress (survival ~ 13% control, 77% free HP, and 85% microencapsulated HP) and improved their growth and reproduction rate. Thus, microencapsulation appears to be a good alternative to maintain hydrolysates stability during storage, showing bioactivity in C. elegans.This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. We also thank the Rio Grande do Sul State Research Foundation (FAPERGS) (17/2551-0000916-9), Brazilian Scientific and Technological National Council (CNPq) and Electron Microscopy Center of South (CEME-SUL) at Federal University of Rio Grande (FURG) for SEM analysis as well as the Agencia Estatal de Investigación (AEI, Spain) and Fondo Europeo de Desarrollo Regional (FEDER), through project NANOALIVAL AGL2017-84161. J. M. Monserrat is a productivity research fellow from the Brazilian CNPq (process number PQ 308539/2016-8).Peer reviewedElsevierCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)Universidade Federal do Rio Grande do SulConselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/251701reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84161-C2-1-Rhttps://doi.org/10.1016/j.foodchem.2021.130380Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2517012026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization, stability, and in vivo effects in Caenorhabditis elegans of microencapsulated protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts
title Characterization, stability, and in vivo effects in Caenorhabditis elegans of microencapsulated protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts
spellingShingle Characterization, stability, and in vivo effects in Caenorhabditis elegans of microencapsulated protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts
Oliveira Lima, Karina
Fish protein hydrolysates
Spray drying
Stability
C. elegans
Physiological effects
Oxidative stress
title_short Characterization, stability, and in vivo effects in Caenorhabditis elegans of microencapsulated protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts
title_full Characterization, stability, and in vivo effects in Caenorhabditis elegans of microencapsulated protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts
title_fullStr Characterization, stability, and in vivo effects in Caenorhabditis elegans of microencapsulated protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts
title_full_unstemmed Characterization, stability, and in vivo effects in Caenorhabditis elegans of microencapsulated protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts
title_sort Characterization, stability, and in vivo effects in Caenorhabditis elegans of microencapsulated protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts
dc.creator.none.fl_str_mv Oliveira Lima, Karina
Alemán, Ailén
López Caballero, M. Elvira
Gómez Guillén, M. C.
Montero García, Pilar
Prentice, Carlos
Taipe Huisa, Andy Joel
Monserrat, José Maria
author Oliveira Lima, Karina
author_facet Oliveira Lima, Karina
Alemán, Ailén
López Caballero, M. Elvira
Gómez Guillén, M. C.
Montero García, Pilar
Prentice, Carlos
Taipe Huisa, Andy Joel
Monserrat, José Maria
author_role author
author2 Alemán, Ailén
López Caballero, M. Elvira
Gómez Guillén, M. C.
Montero García, Pilar
Prentice, Carlos
Taipe Huisa, Andy Joel
Monserrat, José Maria
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Universidade Federal do Rio Grande do Sul
Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fish protein hydrolysates
Spray drying
Stability
C. elegans
Physiological effects
Oxidative stress
topic Fish protein hydrolysates
Spray drying
Stability
C. elegans
Physiological effects
Oxidative stress
description This study aimed to microencapsulate protein hydrolysates from stripped weakfish (Cynoscion guatucupa) industrial byproducts produced by Alcalase (HA) and Protamex (HP) by spray drying, using maltodextrin as wall material. The physicochemical characteristics, and in vitro antioxidant and Angiotensin-I converting enzyme-inhibitory activities were evaluated during storage. Both microencapsulated hydrolysates showed spherical shape (~3.6 µm particle diameter), low water activity (<0.155) during storage and reduced hygroscopicity (~30%) compared to the free hydrolysate. Infrared spectroscopy evidenced the maltodextrin-hydrolysate interaction. Based on the in vitro results, nematoid C. elegans in L1 larval stage were treated with free and microencapsulated HP, which demonstrated a protective effect on nematoid exposed to oxidative stress (survival ~ 13% control, 77% free HP, and 85% microencapsulated HP) and improved their growth and reproduction rate. Thus, microencapsulation appears to be a good alternative to maintain hydrolysates stability during storage, showing bioactivity in C. elegans.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/251701
url http://hdl.handle.net/10261/251701
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84161-C2-1-R
https://doi.org/10.1016/j.foodchem.2021.130380
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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