Determination of water-and fat-soluble vitamins in seawater samples by liquid chromatography-mass spectrometry after simultaneous extraction
This manuscript presents the determination of water- and fat-soluble vitamins in seawater samples after being simultaneously extracted in a single solid-phase extraction procedure. The determination of water- (i.e., B1, B2, B3, B5, B6, B7, B9, and B12) and fat- (i.e., A (as retinal, retinol, and ret...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL) |
| Repositorio: | r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante |
| OAI Identifier: | oai:dnet:isabial_____::60180098a260cbcc1f7d307c8e41abe6 |
| Acceso en línea: | https://isabial.portalinvestigacion.com/publicaciones12598 https://www.sciencedirect.com/science/article/pii/S0889157525007367?pes=vor&utm_source=clarivate&getft_integrator=clarivate |
| Access Level: | acceso abierto |
| Palabra clave: | Water-soluble vitamin Fat-soluble vitamin Solid-phase extraction Liquid chromatography Seawater samples Food supplement |
| Sumario: | This manuscript presents the determination of water- and fat-soluble vitamins in seawater samples after being simultaneously extracted in a single solid-phase extraction procedure. The determination of water- (i.e., B1, B2, B3, B5, B6, B7, B9, and B12) and fat- (i.e., A (as retinal, retinol, and retinoic acid), D2, D3, E (as alpha-, delta and gamma-tocopherol), K1, K2, and K3) soluble vitamins in seawater by liquid chromatography/triple quadrupole mass spectrometry is proposed by two analytical methods. The analytical methods enabled the achievement of low limits of quantification, with values ranging from 0.01 to 0.1 mu g kg-1 and of 0.5 mu g kg-1 for water- and fat-soluble vitamins, respectively. The analysis was conducted using 1 L of seawater, which was subjected to solid-phase extraction, eluted with 40 mL of methanol, evaporated to dryness and reconstituted with 1 g of methanol. The analysis of both filtered and unfiltered seawater samples was successfully carried out. Therefore, the developed methods allow the determination of water- and fat-soluble vitamins in seawater samples, the latter being determined for the first time. In addition, this provides valuable information for mapping the environmental variability of vitamins in the sea, as well as for consumers of seawater-based food supplements. |
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