Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology

33 p.-4 fig.-2 tab.-5 fig. supl.

Detalles Bibliográficos
Autores: Hakalin, Neumara L. S., Molina-Gutiérrez, María, Prieto Orzanco, Alicia, Martínez, María Jesús
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::02aaf0299eaab6fcdeaa42941befdc5a
Acceso en línea:http://hdl.handle.net/10261/164737
Access Level:acceso abierto
Palabra clave:Optimization
Versatile lipase
Phytosterol esters
Enzymatic esterification
OPE
LDL-cholesterol
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spelling Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodologyHakalin, Neumara L. S.Molina-Gutiérrez, MaríaPrieto Orzanco, AliciaMartínez, María JesúsOptimizationVersatile lipasePhytosterol estersEnzymatic esterificationOPELDL-cholesterol33 p.-4 fig.-2 tab.-5 fig. supl.The esters of β-sitostanol and fatty acids are known for their effect as cholesterol-lowering agents. In this work, the efficiency of three lipases as biocatalysts of the esterification of β-sitostanol and C16 and C18 fatty acids was compared. The sterol esterase of Ophiostoma piceae (OPEr) yielded the highest esterification rates and was selected for further optimization of the reaction. The effects of four parameters (temperature, enzymatic dosage, acyl donor concentration, and reaction time) on ester synthesis were investigated and the process conditions were optimized using response surface methodology (RSM). The best conditions for esterification for each fatty acid were predicted using a second-order model, and experimentally validated. Very high esterification efficiencies (86–97%) were observed using the predicted values for the four variables. This approach was shown to be suitable for optimizing the enzymatic production of β-sitostanol esters, which represents a green alternative to the chemical synthesis of these dietary complements.This work has been funded by projects S2013/MAE-2907 from Comunidad de Madrid and and BIO2015-68387-R from MINECO/FEDER.Peer reviewedElsevierComunidad de MadridMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/164737reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-68387-Rhttps://doi.org/10.1016/j.foodchem.2018.04.031Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::02aaf0299eaab6fcdeaa42941befdc5a2026-05-22T06:33:51Z
dc.title.none.fl_str_mv Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology
title Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology
spellingShingle Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology
Hakalin, Neumara L. S.
Optimization
Versatile lipase
Phytosterol esters
Enzymatic esterification
OPE
LDL-cholesterol
title_short Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology
title_full Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology
title_fullStr Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology
title_full_unstemmed Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology
title_sort Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology
dc.creator.none.fl_str_mv Hakalin, Neumara L. S.
Molina-Gutiérrez, María
Prieto Orzanco, Alicia
Martínez, María Jesús
author Hakalin, Neumara L. S.
author_facet Hakalin, Neumara L. S.
Molina-Gutiérrez, María
Prieto Orzanco, Alicia
Martínez, María Jesús
author_role author
author2 Molina-Gutiérrez, María
Prieto Orzanco, Alicia
Martínez, María Jesús
author2_role author
author
author
dc.contributor.none.fl_str_mv Comunidad de Madrid
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Optimization
Versatile lipase
Phytosterol esters
Enzymatic esterification
OPE
LDL-cholesterol
topic Optimization
Versatile lipase
Phytosterol esters
Enzymatic esterification
OPE
LDL-cholesterol
description 33 p.-4 fig.-2 tab.-5 fig. supl.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/164737
url http://hdl.handle.net/10261/164737
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-68387-R
https://doi.org/10.1016/j.foodchem.2018.04.031
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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