Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions

Mycoplasma pneumoniae (MPN) is a bacterial pathogen that primarily causes atypical pneumonia. It cannot synthesize certain essential lipids and therefore relies on the host for their acquisition to survive. MPN has been detected in increased amounts within ruptured atherosclerotic plaques. In this w...

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Detalles Bibliográficos
Autores: Vizarraga, David, Marcos, Marina, Rotllan, Noemi, Martín, Jesús, Santos, David, Camacho, Mercedes, Soto, Begoña, Velasco-Reniu, Lorena, Guerra, Pablo, Pareja, Félix, Collantes, María, Wu, Wanlu, Rodríguez-Arce, Irene, Serrano, Luis, Piñol, Jaume, Fita, Ignacio, Escolà-Gil, Joan Carles
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/421155
Acceso en línea:http://hdl.handle.net/10261/421155
https://api.elsevier.com/content/abstract/scopus_id/105025061980
Access Level:acceso abierto
Palabra clave:Bacterial techniques and applications
Cryoelectron microscopy
Dyslipidaemias
Lipoproteins
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spelling Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesionsVizarraga, DavidMarcos, MarinaRotllan, NoemiMartín, JesúsSantos, DavidCamacho, MercedesSoto, BegoñaVelasco-Reniu, LorenaGuerra, PabloPareja, FélixCollantes, MaríaWu, WanluRodríguez-Arce, IreneSerrano, LuisPiñol, JaumeFita, IgnacioEscolà-Gil, Joan CarlesBacterial techniques and applicationsCryoelectron microscopyDyslipidaemiasLipoproteinsMycoplasma pneumoniae (MPN) is a bacterial pathogen that primarily causes atypical pneumonia. It cannot synthesize certain essential lipids and therefore relies on the host for their acquisition to survive. MPN has been detected in increased amounts within ruptured atherosclerotic plaques. In this work, we show that the protein P116 facilitates cholesterol acquisition from LDL, HDL and various cell types. Targeting P116’s C-terminal domain with a monoclonal antibody inhibits cholesterol acquisition and bacterial growth in vitro. Phase contrast epifluorescence microscopy of human arteries reveals that this antibody blocks MPN binding to atherosclerotic lesions ex vivo. Additionally, an MPN chassis injected into hyperlipidemic female mice localizes to the liver and atherosclerotic plaques. Here, we report that P116 plays a role in extracting essential lipids from lipoproteins and host cells and regulates MPN localization to atheromatous plaques. The study highlights MPN’s potential as a tool for targeting atherosclerotic lesions and fatty liver.This work was partly funded by the Instituto de Salud Carlos III and FEDER “Una manera de hacer Europa” grant PI2300232 (to J.E.-G.). N.R. was funded by Agencia Estatal de Investigación (AEI/10.13039/501100011033 and CNS2023-144119) within the Subprograma Ramón y Cajal (RYC-201722879) and by MICINN-Spain grant PID2022-137186OB-I00. CIBERDEM is an Instituto de Salud Carlos III project. I.F. and J.P. were funded by MICINN-Spain grant PID2021-125632OB-C21 and PID2021-125632OB-C22. The authors acknowledge funding from Project, IU16-014045 (CRYO-TEM) from Generalitat de Catalunya, and by “ERDF A way of making Europe”, by the European Union.Peer reviewedSpringer NatureInstituto de Salud Carlos IIIEuropean CommissionAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Generalitat de CatalunyaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/421155https://api.elsevier.com/content/abstract/scopus_id/105025061980reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137186OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-125632OB-C21info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-125632OB-C22Download of the data available on the platform https://dataverse.csuc.cat/previewurl.xhtml?token=c28f573c-18ee-45f6-a3d8-323087d1342dhttps://doi.org/10.1038/s41467-025-66129-5Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4211552026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions
title Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions
spellingShingle Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions
Vizarraga, David
Bacterial techniques and applications
Cryoelectron microscopy
Dyslipidaemias
Lipoproteins
title_short Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions
title_full Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions
title_fullStr Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions
title_full_unstemmed Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions
title_sort Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions
dc.creator.none.fl_str_mv Vizarraga, David
Marcos, Marina
Rotllan, Noemi
Martín, Jesús
Santos, David
Camacho, Mercedes
Soto, Begoña
Velasco-Reniu, Lorena
Guerra, Pablo
Pareja, Félix
Collantes, María
Wu, Wanlu
Rodríguez-Arce, Irene
Serrano, Luis
Piñol, Jaume
Fita, Ignacio
Escolà-Gil, Joan Carles
author Vizarraga, David
author_facet Vizarraga, David
Marcos, Marina
Rotllan, Noemi
Martín, Jesús
Santos, David
Camacho, Mercedes
Soto, Begoña
Velasco-Reniu, Lorena
Guerra, Pablo
Pareja, Félix
Collantes, María
Wu, Wanlu
Rodríguez-Arce, Irene
Serrano, Luis
Piñol, Jaume
Fita, Ignacio
Escolà-Gil, Joan Carles
author_role author
author2 Marcos, Marina
Rotllan, Noemi
Martín, Jesús
Santos, David
Camacho, Mercedes
Soto, Begoña
Velasco-Reniu, Lorena
Guerra, Pablo
Pareja, Félix
Collantes, María
Wu, Wanlu
Rodríguez-Arce, Irene
Serrano, Luis
Piñol, Jaume
Fita, Ignacio
Escolà-Gil, Joan Carles
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
European Commission
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Generalitat de Catalunya
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacterial techniques and applications
Cryoelectron microscopy
Dyslipidaemias
Lipoproteins
topic Bacterial techniques and applications
Cryoelectron microscopy
Dyslipidaemias
Lipoproteins
description Mycoplasma pneumoniae (MPN) is a bacterial pathogen that primarily causes atypical pneumonia. It cannot synthesize certain essential lipids and therefore relies on the host for their acquisition to survive. MPN has been detected in increased amounts within ruptured atherosclerotic plaques. In this work, we show that the protein P116 facilitates cholesterol acquisition from LDL, HDL and various cell types. Targeting P116’s C-terminal domain with a monoclonal antibody inhibits cholesterol acquisition and bacterial growth in vitro. Phase contrast epifluorescence microscopy of human arteries reveals that this antibody blocks MPN binding to atherosclerotic lesions ex vivo. Additionally, an MPN chassis injected into hyperlipidemic female mice localizes to the liver and atherosclerotic plaques. Here, we report that P116 plays a role in extracting essential lipids from lipoproteins and host cells and regulates MPN localization to atheromatous plaques. The study highlights MPN’s potential as a tool for targeting atherosclerotic lesions and fatty liver.
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/421155
https://api.elsevier.com/content/abstract/scopus_id/105025061980
url http://hdl.handle.net/10261/421155
https://api.elsevier.com/content/abstract/scopus_id/105025061980
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137186OB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-125632OB-C21
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-125632OB-C22
Download of the data available on the platform https://dataverse.csuc.cat/previewurl.xhtml?token=c28f573c-18ee-45f6-a3d8-323087d1342d
https://doi.org/10.1038/s41467-025-66129-5
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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