2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA

[EN]Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where...

ver descrição completa

Detalhes bibliográficos
Autores: Haugland, Marius M., El-Sagheer, Afaf H., Porter, Rachel J., Peña González, Javier, Brown, Tom, Anderson, Edward A., Lovett, Janet E.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/166914
Acesso em linha:http://hdl.handle.net/10366/166914
Access Level:acceso abierto
Palavra-chave:Biopolymers
Electron paramagnetic resonance spectroscopy
Genetics
Labeling
Quantum mechanics
DNA
id ES_6f8c014a2d3e92cc5db458076d1ff424
oai_identifier_str oai:gredos.usal.es:10366/166914
network_acronym_str ES
network_name_str España
repository_id_str
spelling 2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNAHaugland, Marius M.El-Sagheer, Afaf H.Porter, Rachel J.Peña González, JavierBrown, TomAnderson, Edward A.Lovett, Janet E.BiopolymersElectron paramagnetic resonance spectroscopyGeneticsLabelingQuantum mechanicsDNA[EN]Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide–alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron–electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.ACS202520252016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/166914reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1669142026-06-07T06:28:51Z
dc.title.none.fl_str_mv 2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA
title 2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA
spellingShingle 2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA
Haugland, Marius M.
Biopolymers
Electron paramagnetic resonance spectroscopy
Genetics
Labeling
Quantum mechanics
DNA
title_short 2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA
title_full 2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA
title_fullStr 2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA
title_full_unstemmed 2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA
title_sort 2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA
dc.creator.none.fl_str_mv Haugland, Marius M.
El-Sagheer, Afaf H.
Porter, Rachel J.
Peña González, Javier
Brown, Tom
Anderson, Edward A.
Lovett, Janet E.
author Haugland, Marius M.
author_facet Haugland, Marius M.
El-Sagheer, Afaf H.
Porter, Rachel J.
Peña González, Javier
Brown, Tom
Anderson, Edward A.
Lovett, Janet E.
author_role author
author2 El-Sagheer, Afaf H.
Porter, Rachel J.
Peña González, Javier
Brown, Tom
Anderson, Edward A.
Lovett, Janet E.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biopolymers
Electron paramagnetic resonance spectroscopy
Genetics
Labeling
Quantum mechanics
DNA
topic Biopolymers
Electron paramagnetic resonance spectroscopy
Genetics
Labeling
Quantum mechanics
DNA
description [EN]Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide–alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron–electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.
publishDate 2016
dc.date.none.fl_str_mv 2016
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/166914
url http://hdl.handle.net/10366/166914
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ACS
publisher.none.fl_str_mv ACS
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410503391969280
score 15,811543

Registos relacionados