2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA

[EN]Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where...

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Detalles Bibliográficos
Autores: Haugland, Marius M., El-Sagheer, Afaf H., Porter, Rachel J., Peña González, Javier, Brown, Tom, Anderson, Edward A., Lovett, Janet E.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/166914
Acceso en línea:http://hdl.handle.net/10366/166914
Access Level:acceso abierto
Palabra clave:Biopolymers
Electron paramagnetic resonance spectroscopy
Genetics
Labeling
Quantum mechanics
DNA
Descripción
Sumario:[EN]Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide–alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron–electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.

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