The Biosynthesis of Non-Endogenous Apocarotenoids in Transgenic Nicotiana glauca

Crocins are high-value compounds with industrial and food applications. Saffron is currently the main source of these soluble pigments, but its high market price hinders its use by sectors, such as pharmaceutics. Enzymes involved in the production of these compounds have been identified in saffron,...

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Autores: Huang, Xin, Morote, Lucía, Zhu, Changfu, Ahrazem, Oussama, Capell Capell, Teresa, Christou, Paul, Gómez-Gómez, Lourdes
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/84470
Acceso en línea:https://doi.org/10.3390/metabo12070575
http://hdl.handle.net/10459.1/84470
Access Level:acceso abierto
Palabra clave:Apocarotenoids
CsCCD2L
BdCCD4.1
Crocins
Nicotiana glauca
Metabolic engineering
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spelling The Biosynthesis of Non-Endogenous Apocarotenoids in Transgenic Nicotiana glaucaHuang, XinMorote, LucíaZhu, ChangfuAhrazem, OussamaCapell Capell, TeresaChristou, PaulGómez-Gómez, LourdesApocarotenoidsCsCCD2LBdCCD4.1CrocinsNicotiana glaucaMetabolic engineeringCrocins are high-value compounds with industrial and food applications. Saffron is currently the main source of these soluble pigments, but its high market price hinders its use by sectors, such as pharmaceutics. Enzymes involved in the production of these compounds have been identified in saffron, Buddleja, and gardenia. In this study, the enzyme from Buddleja, BdCCD4.1, was constitutively expressed in Nicotiana glauca, a tobacco species with carotenoid-pigmented petals. The transgenic lines produced significant levels of crocins in their leaves and petals. However, the accumulation of crocins was, in general, higher in the leaves than in the petals, reaching almost 302 µg/g DW. The production of crocins was associated with decreased levels of endogenous carotenoids, mainly β-carotene. The stability of crocins in leaf and petal tissues was evaluated after three years of storage, showing an average reduction of 58.06 ± 2.20% in the petals, and 78.37 ± 5.08% in the leaves. This study illustrates the use of BdCCD4.1 as an effective tool for crocin production in N. glauca and how the tissue has an important impact on the stability of produced high-value metabolites during storage.This work was supported by grants BIO2016-77000-R from the Spanish Ministerio de Ciencia; Innovación y Universidades and SBPLY/17/180501/000234 from the Junta de Comunidades de Castilla-La Mancha (co-financed European Union FEDER funds); the National Natural Science Foundation of China (31870278); and the Spanish Ministry of Economy and Competitiveness (MINECO), Spain (RTI2018–097613-B-I00; PGC2018–097655-B-I00). C.Z. and L.G.G. are participants of the European COST action CA15136 (EUROCAROTEN) and Programa Estatal de Investigación Científica y Técnica de excelencia, Spain (BIO2015–71703-REDT and BIO2017–90877-REDT).MDPI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3390/metabo12070575http://hdl.handle.net/10459.1/84470reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097613-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-097655-B-I00Reproducció del document publicat a https://doi.org/10.3390/metabo12070575Metabolites, 2022, vol. 12, núm. 7, art. 575.cc-by (c) Xin Huang et al., 2022info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/844702026-06-24T12:42:17Z
dc.title.none.fl_str_mv The Biosynthesis of Non-Endogenous Apocarotenoids in Transgenic Nicotiana glauca
title The Biosynthesis of Non-Endogenous Apocarotenoids in Transgenic Nicotiana glauca
spellingShingle The Biosynthesis of Non-Endogenous Apocarotenoids in Transgenic Nicotiana glauca
Huang, Xin
Apocarotenoids
CsCCD2L
BdCCD4.1
Crocins
Nicotiana glauca
Metabolic engineering
title_short The Biosynthesis of Non-Endogenous Apocarotenoids in Transgenic Nicotiana glauca
title_full The Biosynthesis of Non-Endogenous Apocarotenoids in Transgenic Nicotiana glauca
title_fullStr The Biosynthesis of Non-Endogenous Apocarotenoids in Transgenic Nicotiana glauca
title_full_unstemmed The Biosynthesis of Non-Endogenous Apocarotenoids in Transgenic Nicotiana glauca
title_sort The Biosynthesis of Non-Endogenous Apocarotenoids in Transgenic Nicotiana glauca
dc.creator.none.fl_str_mv Huang, Xin
Morote, Lucía
Zhu, Changfu
Ahrazem, Oussama
Capell Capell, Teresa
Christou, Paul
Gómez-Gómez, Lourdes
author Huang, Xin
author_facet Huang, Xin
Morote, Lucía
Zhu, Changfu
Ahrazem, Oussama
Capell Capell, Teresa
Christou, Paul
Gómez-Gómez, Lourdes
author_role author
author2 Morote, Lucía
Zhu, Changfu
Ahrazem, Oussama
Capell Capell, Teresa
Christou, Paul
Gómez-Gómez, Lourdes
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Apocarotenoids
CsCCD2L
BdCCD4.1
Crocins
Nicotiana glauca
Metabolic engineering
topic Apocarotenoids
CsCCD2L
BdCCD4.1
Crocins
Nicotiana glauca
Metabolic engineering
description Crocins are high-value compounds with industrial and food applications. Saffron is currently the main source of these soluble pigments, but its high market price hinders its use by sectors, such as pharmaceutics. Enzymes involved in the production of these compounds have been identified in saffron, Buddleja, and gardenia. In this study, the enzyme from Buddleja, BdCCD4.1, was constitutively expressed in Nicotiana glauca, a tobacco species with carotenoid-pigmented petals. The transgenic lines produced significant levels of crocins in their leaves and petals. However, the accumulation of crocins was, in general, higher in the leaves than in the petals, reaching almost 302 µg/g DW. The production of crocins was associated with decreased levels of endogenous carotenoids, mainly β-carotene. The stability of crocins in leaf and petal tissues was evaluated after three years of storage, showing an average reduction of 58.06 ± 2.20% in the petals, and 78.37 ± 5.08% in the leaves. This study illustrates the use of BdCCD4.1 as an effective tool for crocin production in N. glauca and how the tissue has an important impact on the stability of produced high-value metabolites during storage.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/metabo12070575
http://hdl.handle.net/10459.1/84470
url https://doi.org/10.3390/metabo12070575
http://hdl.handle.net/10459.1/84470
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097613-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-097655-B-I00
Reproducció del document publicat a https://doi.org/10.3390/metabo12070575
Metabolites, 2022, vol. 12, núm. 7, art. 575.
dc.rights.none.fl_str_mv cc-by (c) Xin Huang et al., 2022
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Xin Huang et al., 2022
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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