Identification and characterization of apocarotenoid modifiers and carotenogenic enzymes for biosynthesis of crocins in Buddleja davidii flowers

Crocetin biosynthesis in Buddleja davidii flowers proceeds through a zeaxanthin cleavage pathway catalyzed by two carotenoid cleavage dioxygenases (BdCCD4.1 and BdCCD4.3), followed by oxidation and glucosylation reactions that lead to the production of crocins. We isolated and analyzed the expressio...

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Detalles Bibliográficos
Autores: Diretto, Gianfranco, López Jiménez, Alberto José, Ahrazem El Kadiri, Oussama, Frusciante, Sarah, Song, Jingyuan, Rubio Moraga, Ángela, Gómez Gómez, María Lourdes
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/35247
Acceso en línea:https://hdl.handle.net/10578/35247
Access Level:acceso abierto
Palabra clave:Aldehyde dehydrogenase
Apocarotenoids
Carotenoid cleavage dioxygenases
Carotenoids
Crocins
Flower
Glucosyltransferase
Picrocrocin
Descripción
Sumario:Crocetin biosynthesis in Buddleja davidii flowers proceeds through a zeaxanthin cleavage pathway catalyzed by two carotenoid cleavage dioxygenases (BdCCD4.1 and BdCCD4.3), followed by oxidation and glucosylation reactions that lead to the production of crocins. We isolated and analyzed the expression of 12 genes from the carotenoid pathway in B. davidii flowers and identified four candidate genes involved in the biosynthesis of crocins (BdALDH, BdUGT74BC1, BdUGT74BC2, and BdUGT94AA3).

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