Conservation Implications for the Iberian Narrow Endemic Androsace cantabrica (Primulaceae) Using Population Genomics With Target Capture Sequence Data

Androsace cantabrica (Losa & P. Monts.) Kress is a narrow endemic alpine plant restricted to a few high- elevation localities inthe Cantabrian Mountains of northern Spain. Although currently accepted as a distinct species, its close morphological andphylogenetic affinity to related taxa such as...

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Autores: Liang, Ke (Jungle), Shepherd-Clowes, Amelia, Agut, Agustí, Hidalgo, Oriane, Tejero Ibarra, Pablo, Viruel, Juan
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/399357
Acesso em linha:http://hdl.handle.net/10261/399357
Access Level:acceso abierto
Palavra-chave:Alpine ecosystem
Angiosperms353
Conservation genetics
Global warming
Phylogenomics
Population genomics
Threatened species
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spelling Conservation Implications for the Iberian Narrow Endemic Androsace cantabrica (Primulaceae) Using Population Genomics With Target Capture Sequence DataLiang, Ke (Jungle)Shepherd-Clowes, AmeliaAgut, AgustíHidalgo, OrianeTejero Ibarra, PabloViruel, JuanAlpine ecosystemAngiosperms353Conservation geneticsGlobal warmingPhylogenomicsPopulation genomicsThreatened speciesAndrosace cantabrica (Losa & P. Monts.) Kress is a narrow endemic alpine plant restricted to a few high- elevation localities inthe Cantabrian Mountains of northern Spain. Although currently accepted as a distinct species, its close morphological andphylogenetic affinity to related taxa such as Androsace adfinis and Androsace halleri has led to historical uncertainty about itstaxonomic status and evolutionary origin. Here, we use the universal Angiosperms353 target capture kit to generate nuclear andplastid data from A. cantabrica and closely related species in section Aretia. We employ phylogenomic analyses to clarify speciesboundaries and population genomic analyses to inform conservation management, as well as flow cytometry and sequence-based analysis using allelic frequencies to estimate its ploidy level. Phylogenetic analyses based on nuclear loci support A. can-tabrica as a monophyletic clade, distinct from both A. adfinis and the A. halleri and Androsace laggeri clades, although topologicalincongruence with plastid data suggests historical hybridization. Flow cytometry and allelic frequency-based analysis indicatethat A. cantabrica is tetraploid, differentiating it from A. halleri and A. laggeri, which are diploid. Population structure analysesreveal a shallow genetic split between eastern and western groups (FST = 0.04485), with higher genetic diversity observed in theeast. We estimated the species' distribution, population sizes, and threats, and classified it as Vulnerable under IUCN criteriaB1ab(ii,iii) + 2ab(ii,iii). We recommend targeted in situ management, ex situ seed conservation, and the establishment of a micro-reserve. This study illustrates the utility of Angiosperms353 data for resolving both taxonomic questions and conservation strat-egies in polyploid, range-restricted species.This research is a result of the PRIOCONEX project, supported by the “Fundación Biodiversidad” of the Spanish “Ministerio para la transición ecológica y el reto demográfico” and the “Aranzadi Zientzia Elkartea.” K.J.L. received financial sponsorship for fieldwork in Spain from the MSc in “Plantand Fungal Taxonomy, Diversity and Conservation,” jointly organized by Queen Mary University of London and RBG KewPeer reviewedJohn Wiley & SonsMinisterio de Transición Ecológica (España)Sociedad de Ciencias AranzadiQueen Mary University of LondonRoyal Botanical Gardens, KewConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/399357reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1002/ece3.71901Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3993572026-05-22T06:33:51Z
dc.title.none.fl_str_mv Conservation Implications for the Iberian Narrow Endemic Androsace cantabrica (Primulaceae) Using Population Genomics With Target Capture Sequence Data
title Conservation Implications for the Iberian Narrow Endemic Androsace cantabrica (Primulaceae) Using Population Genomics With Target Capture Sequence Data
spellingShingle Conservation Implications for the Iberian Narrow Endemic Androsace cantabrica (Primulaceae) Using Population Genomics With Target Capture Sequence Data
Liang, Ke (Jungle)
Alpine ecosystem
Angiosperms353
Conservation genetics
Global warming
Phylogenomics
Population genomics
Threatened species
title_short Conservation Implications for the Iberian Narrow Endemic Androsace cantabrica (Primulaceae) Using Population Genomics With Target Capture Sequence Data
title_full Conservation Implications for the Iberian Narrow Endemic Androsace cantabrica (Primulaceae) Using Population Genomics With Target Capture Sequence Data
title_fullStr Conservation Implications for the Iberian Narrow Endemic Androsace cantabrica (Primulaceae) Using Population Genomics With Target Capture Sequence Data
title_full_unstemmed Conservation Implications for the Iberian Narrow Endemic Androsace cantabrica (Primulaceae) Using Population Genomics With Target Capture Sequence Data
title_sort Conservation Implications for the Iberian Narrow Endemic Androsace cantabrica (Primulaceae) Using Population Genomics With Target Capture Sequence Data
dc.creator.none.fl_str_mv Liang, Ke (Jungle)
Shepherd-Clowes, Amelia
Agut, Agustí
Hidalgo, Oriane
Tejero Ibarra, Pablo
Viruel, Juan
author Liang, Ke (Jungle)
author_facet Liang, Ke (Jungle)
Shepherd-Clowes, Amelia
Agut, Agustí
Hidalgo, Oriane
Tejero Ibarra, Pablo
Viruel, Juan
author_role author
author2 Shepherd-Clowes, Amelia
Agut, Agustí
Hidalgo, Oriane
Tejero Ibarra, Pablo
Viruel, Juan
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Transición Ecológica (España)
Sociedad de Ciencias Aranzadi
Queen Mary University of London
Royal Botanical Gardens, Kew
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Alpine ecosystem
Angiosperms353
Conservation genetics
Global warming
Phylogenomics
Population genomics
Threatened species
topic Alpine ecosystem
Angiosperms353
Conservation genetics
Global warming
Phylogenomics
Population genomics
Threatened species
description Androsace cantabrica (Losa & P. Monts.) Kress is a narrow endemic alpine plant restricted to a few high- elevation localities inthe Cantabrian Mountains of northern Spain. Although currently accepted as a distinct species, its close morphological andphylogenetic affinity to related taxa such as Androsace adfinis and Androsace halleri has led to historical uncertainty about itstaxonomic status and evolutionary origin. Here, we use the universal Angiosperms353 target capture kit to generate nuclear andplastid data from A. cantabrica and closely related species in section Aretia. We employ phylogenomic analyses to clarify speciesboundaries and population genomic analyses to inform conservation management, as well as flow cytometry and sequence-based analysis using allelic frequencies to estimate its ploidy level. Phylogenetic analyses based on nuclear loci support A. can-tabrica as a monophyletic clade, distinct from both A. adfinis and the A. halleri and Androsace laggeri clades, although topologicalincongruence with plastid data suggests historical hybridization. Flow cytometry and allelic frequency-based analysis indicatethat A. cantabrica is tetraploid, differentiating it from A. halleri and A. laggeri, which are diploid. Population structure analysesreveal a shallow genetic split between eastern and western groups (FST = 0.04485), with higher genetic diversity observed in theeast. We estimated the species' distribution, population sizes, and threats, and classified it as Vulnerable under IUCN criteriaB1ab(ii,iii) + 2ab(ii,iii). We recommend targeted in situ management, ex situ seed conservation, and the establishment of a micro-reserve. This study illustrates the utility of Angiosperms353 data for resolving both taxonomic questions and conservation strat-egies in polyploid, range-restricted species.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/399357
url http://hdl.handle.net/10261/399357
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1002/ece3.71901
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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