Sparse force-bearing bridges between neighboring synaptic vesicles

Most vesicles in the interior of synaptic terminals are clustered in clouds close to active zone regions of the plasma membrane where exocytosis occurs. Electron-dense structures, termed bridges, have been reported between a small minority of pairs of neighboring vesicles within the clouds. Synapsin...

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Autores: Wesseling, John F., Phan, Sébastien, Bushong, Eric A., Siksou, Léa, Marty, Serge, Pérez-Otaño, Isabel, Ellisman, Mark
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/217958
Acceso en línea:http://hdl.handle.net/10261/217958
Access Level:acceso abierto
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spelling Sparse force-bearing bridges between neighboring synaptic vesiclesWesseling, John F.Phan, SébastienBushong, Eric A.Siksou, LéaMarty, SergePérez-Otaño, IsabelEllisman, MarkMost vesicles in the interior of synaptic terminals are clustered in clouds close to active zone regions of the plasma membrane where exocytosis occurs. Electron-dense structures, termed bridges, have been reported between a small minority of pairs of neighboring vesicles within the clouds. Synapsin proteins have been implicated previously, but the existence of the bridges as stable structures in vivo has been questioned. Here we use electron tomography to show that the bridges are present but less frequent in synapsin knockouts compared to wildtype. An analysis of distances between neighbors in wildtype tomograms indicated that the bridges are strong enough to resist centrifugal forces likely induced by fixation with aldehydes. The results confirm that the bridges are stable structures and that synapsin proteins are involved in formation or stabilization.This work was funded by: the US National Institute of Health (NIHGM103412 to ME); the Unión Temporal de Empresas (UTE) project at the Centro de Investigación Médica Aplicada of the Universidad de Navarra; the Spanish Ministry of Science (BFU2009-12160, SAF2013-48983R, and BFU2016-80918R to JFW); a Salvador de Madariaga Visiting Scholarship award to JFW; and the Spanish Jerónimo de Ayanz program to IPO and JFW.Peer reviewedSpringer NatureUniversidad de NavarraMinisterio de Economía y Competitividad (España)Ministerio de Ciencia e Innovación (España)National Institutes of Health (US)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/217958reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2013-48983-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-80918-Rhttps://doi.org/10.1007/s00429-019-01966-xSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2179582026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sparse force-bearing bridges between neighboring synaptic vesicles
title Sparse force-bearing bridges between neighboring synaptic vesicles
spellingShingle Sparse force-bearing bridges between neighboring synaptic vesicles
Wesseling, John F.
title_short Sparse force-bearing bridges between neighboring synaptic vesicles
title_full Sparse force-bearing bridges between neighboring synaptic vesicles
title_fullStr Sparse force-bearing bridges between neighboring synaptic vesicles
title_full_unstemmed Sparse force-bearing bridges between neighboring synaptic vesicles
title_sort Sparse force-bearing bridges between neighboring synaptic vesicles
dc.creator.none.fl_str_mv Wesseling, John F.
Phan, Sébastien
Bushong, Eric A.
Siksou, Léa
Marty, Serge
Pérez-Otaño, Isabel
Ellisman, Mark
author Wesseling, John F.
author_facet Wesseling, John F.
Phan, Sébastien
Bushong, Eric A.
Siksou, Léa
Marty, Serge
Pérez-Otaño, Isabel
Ellisman, Mark
author_role author
author2 Phan, Sébastien
Bushong, Eric A.
Siksou, Léa
Marty, Serge
Pérez-Otaño, Isabel
Ellisman, Mark
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Navarra
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
National Institutes of Health (US)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Most vesicles in the interior of synaptic terminals are clustered in clouds close to active zone regions of the plasma membrane where exocytosis occurs. Electron-dense structures, termed bridges, have been reported between a small minority of pairs of neighboring vesicles within the clouds. Synapsin proteins have been implicated previously, but the existence of the bridges as stable structures in vivo has been questioned. Here we use electron tomography to show that the bridges are present but less frequent in synapsin knockouts compared to wildtype. An analysis of distances between neighbors in wildtype tomograms indicated that the bridges are strong enough to resist centrifugal forces likely induced by fixation with aldehydes. The results confirm that the bridges are stable structures and that synapsin proteins are involved in formation or stabilization.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/217958
url http://hdl.handle.net/10261/217958
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2013-48983-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-80918-R
https://doi.org/10.1007/s00429-019-01966-x
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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