Lipidomic changes of LDL in overweight and moderately hypercholesterolemic subjects taking phytosterol- and omega-3-supplemented milk
The benefits of dietary phytosterols (PhySs) and long-chain n-3 PUFA (omega 3) have been linked to their effects as cholesterol-and triglyceride (TGL)-lowering agents. However, it remains unknown whether these compounds have further metabolic effects on LDL lipid composition. Here, we studied the ef...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau) |
| Repositorio: | r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
| OAI Identifier: | oai:iibsantpau.fundanetsuite.com:p14531 |
| Acceso en línea: | https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=14531 |
| Access Level: | acceso abierto |
| Palabra clave: | atherosclerosis cholesterol triglycerides diet and dietary lipids phospholipids mass spectrometry glycerophospholipids low density lipoprotein-lipid metabolites |
| Sumario: | The benefits of dietary phytosterols (PhySs) and long-chain n-3 PUFA (omega 3) have been linked to their effects as cholesterol-and triglyceride (TGL)-lowering agents. However, it remains unknown whether these compounds have further metabolic effects on LDL lipid composition. Here, we studied the effects of PhyS-or omega 3-supplemented low-fat milk (milk) on the LDL-lipidome. Overweight and moderately hypercholesterolemic subjects (n = 32) were enrolled in a two-arm longitudinal crossover study. Milk (250 ml/day), enriched with either 1.57 g PhyS or 375 mg omega 3 (EPA + DHA), was given to the participants during two sequential 28 day intervention periods. Compared with baseline, PhyS-milk induced a higher reduction in the LDL cholesterol (LDLc) level than omega 3-milk. LDL resistance to oxidation was significantly increased after intervention with PhyS-milk. Changes in TGL and VLDL cholesterol were only evident after omega 3-milk intake. Lipidomic analysis revealed a differential effect of the PhyS-and omega 3-milk interventions on the LDL lipid metabolite pattern. Content in LDL-glycerophospholipids was reduced after PhyS-milk intake, with major changes in phosphatidylcholine (PC) and phosphatidylserine subclasses, whereas omega 3-milk induced signifi cant changes in the long-chain polyunsaturated cholesteryl esters and in the ratio PC36: 5/lysoPC16: 0, associated to a reduced inflammatory activity. In conclusion, daily intake of milk products containing PhyS or omega 3 supplements induce changes in the LDL-lipidome that indicate reduced inflammatory and atherogenic effects, beyond their LDLc-and TGL-lowering effects. |
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