Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches

The labelled of pistachio on food products is mandatory and, as a consequence, the development of suitable analytical methodologies to detect this nut in processed foods is advisable. In this work, two different qPCR assays to detect pistachio, SYBR®Green and locked nucleic acid (LNA) probes, are te...

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Detalles Bibliográficos
Autores: Sanchiz Giraldo, África, Ballesteros Redondo, María Isabel, Martín, Ana, Rueda Muñoz de San Pedro, Julia, Martín Pedrosa, Mercedes, Diéguez, Carmen, Rovira, Mercè, Cuadrado Vives, María Carmen, Linacero De La Fuente, M. Rosario
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/17952
Acceso en línea:https://hdl.handle.net/20.500.14352/17952
Access Level:acceso abierto
Palabra clave:577.2
616.056.3:612.392
634.5
577.213.3.08
Pistachio
Allergen detection
Real time PCR
Processed foods
Biología
Biología molecular (Biología)
Genética
24 Ciencias de la Vida
2415 Biología Molecular
2409 Genética
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oai_identifier_str oai:docta.ucm.es:20.500.14352/17952
network_acronym_str ES
network_name_str España
repository_id_str
spelling Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approachesSanchiz Giraldo, ÁfricaBallesteros Redondo, María IsabelMartín, AnaRueda Muñoz de San Pedro, JuliaMartín Pedrosa, MercedesDiéguez, CarmenRovira, MercèCuadrado Vives, María CarmenLinacero De La Fuente, M. Rosario577.2616.056.3:612.392634.5577.213.3.08PistachioAllergen detectionReal time PCRProcessed foodsBiologíaBiología molecular (Biología)Genética24 Ciencias de la Vida2415 Biología Molecular2409 GenéticaThe labelled of pistachio on food products is mandatory and, as a consequence, the development of suitable analytical methodologies to detect this nut in processed foods is advisable. In this work, two different qPCR assays to detect pistachio, SYBR®Green and locked nucleic acid (LNA) probes, are tested and compared. Pis v allergen coding sequences have been amplified and cloned in different pistachio varieties, and specific primers and probes for each allergen have been designed. According to our results, LNA probe-real time PCR appears to be the most sensitive and specific method, reaching 10 mg/kg of pistachio. The effect of temperature and/or pressure on pistachio DNA detection was also analysed by LNA probe-based qPCR. Data showed a reduced amplificability of pistachio after thermal treatment under pressure, nevertheless, this effect was not observed after boiling. The applicability of this method has been studied by analysing 14 food products and by comparison with a commercial ELISA kit.ElsevierUniversidad Complutense de Madrid20172017-01-0120172017-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://hdl.handle.net/20.500.14352/17952reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2012-39863-C02-01 EFECTO DE PROCESADO TECNOLOGICO COMBINANDO TEMPERATURA, PRESION Y TRATAMIENTOS ENZIMATICOS SOBRE LA REACTIVIDAD ALERGENICA DE FRUTOS SECOS (ANACARDO, PISTACHO Y CASTAÑA)open accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/179522026-06-02T12:44:21Z
dc.title.none.fl_str_mv Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches
title Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches
spellingShingle Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches
Sanchiz Giraldo, África
577.2
616.056.3:612.392
634.5
577.213.3.08
Pistachio
Allergen detection
Real time PCR
Processed foods
Biología
Biología molecular (Biología)
Genética
24 Ciencias de la Vida
2415 Biología Molecular
2409 Genética
title_short Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches
title_full Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches
title_fullStr Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches
title_full_unstemmed Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches
title_sort Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches
dc.creator.none.fl_str_mv Sanchiz Giraldo, África
Ballesteros Redondo, María Isabel
Martín, Ana
Rueda Muñoz de San Pedro, Julia
Martín Pedrosa, Mercedes
Diéguez, Carmen
Rovira, Mercè
Cuadrado Vives, María Carmen
Linacero De La Fuente, M. Rosario
author Sanchiz Giraldo, África
author_facet Sanchiz Giraldo, África
Ballesteros Redondo, María Isabel
Martín, Ana
Rueda Muñoz de San Pedro, Julia
Martín Pedrosa, Mercedes
Diéguez, Carmen
Rovira, Mercè
Cuadrado Vives, María Carmen
Linacero De La Fuente, M. Rosario
author_role author
author2 Ballesteros Redondo, María Isabel
Martín, Ana
Rueda Muñoz de San Pedro, Julia
Martín Pedrosa, Mercedes
Diéguez, Carmen
Rovira, Mercè
Cuadrado Vives, María Carmen
Linacero De La Fuente, M. Rosario
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.2
616.056.3:612.392
634.5
577.213.3.08
Pistachio
Allergen detection
Real time PCR
Processed foods
Biología
Biología molecular (Biología)
Genética
24 Ciencias de la Vida
2415 Biología Molecular
2409 Genética
topic 577.2
616.056.3:612.392
634.5
577.213.3.08
Pistachio
Allergen detection
Real time PCR
Processed foods
Biología
Biología molecular (Biología)
Genética
24 Ciencias de la Vida
2415 Biología Molecular
2409 Genética
description The labelled of pistachio on food products is mandatory and, as a consequence, the development of suitable analytical methodologies to detect this nut in processed foods is advisable. In this work, two different qPCR assays to detect pistachio, SYBR®Green and locked nucleic acid (LNA) probes, are tested and compared. Pis v allergen coding sequences have been amplified and cloned in different pistachio varieties, and specific primers and probes for each allergen have been designed. According to our results, LNA probe-real time PCR appears to be the most sensitive and specific method, reaching 10 mg/kg of pistachio. The effect of temperature and/or pressure on pistachio DNA detection was also analysed by LNA probe-based qPCR. Data showed a reduced amplificability of pistachio after thermal treatment under pressure, nevertheless, this effect was not observed after boiling. The applicability of this method has been studied by analysing 14 food products and by comparison with a commercial ELISA kit.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01
2017
2017-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/17952
url https://hdl.handle.net/20.500.14352/17952
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2012-39863-C02-01 EFECTO DE PROCESADO TECNOLOGICO COMBINANDO TEMPERATURA, PRESION Y TRATAMIENTOS ENZIMATICOS SOBRE LA REACTIVIDAD ALERGENICA DE FRUTOS SECOS (ANACARDO, PISTACHO Y CASTAÑA)
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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