Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe

One hundred and 26 faecal samples from healthy dogs (2009) and 157 faecal samples from healthy humans (2007) from La Rioja region (Spain) were tested to know the carriage of vancomycin-resistant enterococci (VRE). VRE with intrinsic resistance (vanC) were found in 12% of healthy dogs and humans (29...

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Autores: López, M. [0000-0002-3834-4891], Tenorio, C. [0000-0002-8099-1208], Torres, C. [0000-0003-3709-1690]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Universidad de La Rioja (UR)
Repositorio:RIUR. Repositorio Institucional de la Universidad de La Rioja
OAI Identifier:oai:portal.dialnet.es:doc/5bbc68cfb750603269e80ff0
Acceso en línea:https://investigacion.unirioja.es/documentos/5bbc68cfb750603269e80ff0
Access Level:acceso abierto
Palabra clave:Bacteriocin
Healthy human
Pet
Vancomycin-resistant enterococci
Virulence
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spelling Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in EuropeLópez, M. [0000-0002-3834-4891]Tenorio, C. [0000-0002-8099-1208]Torres, C. [0000-0003-3709-1690]BacteriocinHealthy humanPetVancomycin-resistant enterococciVirulenceOne hundred and 26 faecal samples from healthy dogs (2009) and 157 faecal samples from healthy humans (2007) from La Rioja region (Spain) were tested to know the carriage of vancomycin-resistant enterococci (VRE). VRE with intrinsic resistance (vanC) were found in 12% of healthy dogs and humans (29 Enterococcus gallinarum and four Enterococcus casseliflavus). Nevertheless, VRE with acquired mechanism of resistance were not detected among these samples. Four Enterococcus faecalis isolates with vancomycin MIC of 8-16mgL-1 were recovered in human samples, but no single organism with known mechanism of acquired resistance could be identified. These 37 VRE isolates (33 E. gallinarum/E. casseliflavus and four E. faecalis) of dog and human origin were further characterized (PCR detection of antibiotic resistance, virulence and bacteriocin genes). High prevalence of tetracycline resistance was identified (70%), especially among dog isolates harbouring tet(M)±tet(L) genes; erythromycin resistance was also higher among isolates from dogs and they harboured the erm(B) gene, associated with erm(A) gene in one case. Virulence genes were only identified among E. faecalis isolates of human origin (agg, cpd and/or gelE) and never among E. gallinarum/E. casseliflavus of human or dog origin. Five E. gallinarum isolates of dog and three E. faecalis of human origin expressed bacteriocin activity; among them, only one E. faecalis presented activity against Listeria monocytogenes. The bacteriocin structural gene ef1097 was identified in 3 bacteriocin-producing E. faecalis isolates, associated with ent1071 in one of them. © 2012 Blackwell Verlag GmbH.2013info:eu-repo/semantics/articleSubtype: Articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://investigacion.unirioja.es/documentos/5bbc68cfb750603269e80ff0reponame:RIUR. Repositorio Institucional de la Universidad de La Riojainstname:Universidad de La Rioja (UR)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1111/J.1863-2378.2012.01502.Xinfo:eu-repo/semantics/altIdentifier/wos/WOS:000314469200007info:eu-repo/semantics/altIdentifier/pissn/1863-1959Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe, 2013, vol. 60, núm. 2, pág. 160-167info:eu-repo/semantics/openAccessoai:portal.dialnet.es:doc/5bbc68cfb750603269e80ff02026-06-14T12:47:17Z
dc.title.none.fl_str_mv Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe
title Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe
spellingShingle Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe
López, M. [0000-0002-3834-4891]
Bacteriocin
Healthy human
Pet
Vancomycin-resistant enterococci
Virulence
title_short Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe
title_full Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe
title_fullStr Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe
title_full_unstemmed Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe
title_sort Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe
dc.creator.none.fl_str_mv López, M. [0000-0002-3834-4891]
Tenorio, C. [0000-0002-8099-1208]
Torres, C. [0000-0003-3709-1690]
author López, M. [0000-0002-3834-4891]
author_facet López, M. [0000-0002-3834-4891]
Tenorio, C. [0000-0002-8099-1208]
Torres, C. [0000-0003-3709-1690]
author_role author
author2 Tenorio, C. [0000-0002-8099-1208]
Torres, C. [0000-0003-3709-1690]
author2_role author
author
dc.subject.none.fl_str_mv Bacteriocin
Healthy human
Pet
Vancomycin-resistant enterococci
Virulence
topic Bacteriocin
Healthy human
Pet
Vancomycin-resistant enterococci
Virulence
description One hundred and 26 faecal samples from healthy dogs (2009) and 157 faecal samples from healthy humans (2007) from La Rioja region (Spain) were tested to know the carriage of vancomycin-resistant enterococci (VRE). VRE with intrinsic resistance (vanC) were found in 12% of healthy dogs and humans (29 Enterococcus gallinarum and four Enterococcus casseliflavus). Nevertheless, VRE with acquired mechanism of resistance were not detected among these samples. Four Enterococcus faecalis isolates with vancomycin MIC of 8-16mgL-1 were recovered in human samples, but no single organism with known mechanism of acquired resistance could be identified. These 37 VRE isolates (33 E. gallinarum/E. casseliflavus and four E. faecalis) of dog and human origin were further characterized (PCR detection of antibiotic resistance, virulence and bacteriocin genes). High prevalence of tetracycline resistance was identified (70%), especially among dog isolates harbouring tet(M)±tet(L) genes; erythromycin resistance was also higher among isolates from dogs and they harboured the erm(B) gene, associated with erm(A) gene in one case. Virulence genes were only identified among E. faecalis isolates of human origin (agg, cpd and/or gelE) and never among E. gallinarum/E. casseliflavus of human or dog origin. Five E. gallinarum isolates of dog and three E. faecalis of human origin expressed bacteriocin activity; among them, only one E. faecalis presented activity against Listeria monocytogenes. The bacteriocin structural gene ef1097 was identified in 3 bacteriocin-producing E. faecalis isolates, associated with ent1071 in one of them. © 2012 Blackwell Verlag GmbH.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
Subtype: Article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://investigacion.unirioja.es/documentos/5bbc68cfb750603269e80ff0
url https://investigacion.unirioja.es/documentos/5bbc68cfb750603269e80ff0
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/J.1863-2378.2012.01502.X
info:eu-repo/semantics/altIdentifier/wos/WOS:000314469200007
info:eu-repo/semantics/altIdentifier/pissn/1863-1959
Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe, 2013, vol. 60, núm. 2, pág. 160-167
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:RIUR. Repositorio Institucional de la Universidad de La Rioja
instname:Universidad de La Rioja (UR)
instname_str Universidad de La Rioja (UR)
reponame_str RIUR. Repositorio Institucional de la Universidad de La Rioja
collection RIUR. Repositorio Institucional de la Universidad de La Rioja
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