Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region

Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% a...

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Detalles Bibliográficos
Autores: Casas Flecha, Inmaculada, Avellón, Ana, Mosquera Gutierrez, Maria del Mar, Jabado, O, Echevarria, Juan Emilio, Campos, R H, Rewers, M, Perez-Breña, Pilar, Lipkin, W I, Palacios, G
Tipo de recurso: artículo
Fecha de publicación:2005
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/8278
Acceso en línea:http://hdl.handle.net/20.500.12105/8278
Access Level:acceso abierto
Palabra clave:Adenovirus Infections, Human
Adenoviruses, Human
Adolescent
Adult
Capsid Proteins
Child
Child, Preschool
DNA, Viral
Genome, Viral
Humans
Infant
Infant, Newborn
Middle Aged
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Serotyping
Sequence Analysis, DNA
Descripción
Sumario:Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.

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