Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish.

Zebrafish have the capacity to regenerate most of its organs upon injury, including the heart. Due to its amenability for genetic manipulation, the zebrafish is an excellent model organism to study the cellular and molecular mechanisms promoting heart regeneration. Several cardiac injury models have...

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Bibliographic Details
Authors: Marques, Ines Joao, Sanz-Morejon, Andres, Mercader, Nadia
Format: article
Publication Date:2021
Country:España
Institution:Instituto de Salud Carlos III (ISCIII)
Repository:Repisalud
Language:English
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/13317
Online Access:http://hdl.handle.net/20.500.12105/13317
Access Level:Open access
Keyword:Disease Models, Animal
Regeneration
Ventricular Remodeling
Animals
Cardiac Surgical Procedures
Cell Proliferation
Cryosurgery
Heart
Heart Injuries
Zebrafish
Description
Summary:Zebrafish have the capacity to regenerate most of its organs upon injury, including the heart. Due to its amenability for genetic manipulation, the zebrafish is an excellent model organism to study the cellular and molecular mechanisms promoting heart regeneration. Several cardiac injury models have been developed in zebrafish, including ventricular resection, genetic ablation, and ventricular cryoinjury. This chapter provides a detailed protocol of zebrafish ventricular cryoinjury and highlights factors and critical steps to be considered when performing this method.