Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish.

Zebrafish have the capacity to regenerate most of its organs upon injury, including the heart. Due to its amenability for genetic manipulation, the zebrafish is an excellent model organism to study the cellular and molecular mechanisms promoting heart regeneration. Several cardiac injury models have...

Descripción completa

Detalles Bibliográficos
Autores: Marques, Ines Joao, Sanz-Morejon, Andres, Mercader, Nadia
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/13317
Acceso en línea:http://hdl.handle.net/20.500.12105/13317
Access Level:acceso abierto
Palabra clave:Disease Models, Animal
Regeneration
Ventricular Remodeling
Animals
Cardiac Surgical Procedures
Cell Proliferation
Cryosurgery
Heart
Heart Injuries
Zebrafish
Descripción
Sumario:Zebrafish have the capacity to regenerate most of its organs upon injury, including the heart. Due to its amenability for genetic manipulation, the zebrafish is an excellent model organism to study the cellular and molecular mechanisms promoting heart regeneration. Several cardiac injury models have been developed in zebrafish, including ventricular resection, genetic ablation, and ventricular cryoinjury. This chapter provides a detailed protocol of zebrafish ventricular cryoinjury and highlights factors and critical steps to be considered when performing this method.