First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6

A novel electrochemical bioplatform for Pru du 6 determination, one of the most abundant and allergenic almond proteins, was developed to detect traces of almonds in processed foods. The bioplatform combines the application of a sandwich immunoassay on the surface of magnetic beads using specific an...

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Detalles Bibliográficos
Autores: Civera, Alba, Tvorynska, Sofiia, Gamella Carballo, María, Torrente Rodríguez, Rebeca Magnolia, Pedrero Muñoz, María, Galan-Malo, Patricia, Mata, Luis, Sanchez, Lourdes, Barek, Jiri, Pingarrón Carrazón, José Manuel, Campuzano Ruiz, Susana, Perez, Maria D.
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/114295
Acceso en línea:https://hdl.handle.net/20.500.14352/114295
Access Level:acceso abierto
Palabra clave:543
Electrochemical immunoplatform
Almond
Pru du 6
Incurred cookies
Biosensor
Química analítica (Química)
2301.04 Análisis Electroquímico
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oai_identifier_str oai:docta.ucm.es:20.500.14352/114295
network_acronym_str ES
network_name_str España
repository_id_str
spelling First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6Civera, AlbaTvorynska, SofiiaGamella Carballo, MaríaTorrente Rodríguez, Rebeca MagnoliaPedrero Muñoz, MaríaGalan-Malo, PatriciaMata, LuisSanchez, LourdesBarek, JiriPingarrón Carrazón, José ManuelCampuzano Ruiz, SusanaPerez, Maria D.543Electrochemical immunoplatformAlmondPru du 6Incurred cookiesBiosensorQuímica analítica (Química)2301.04 Análisis ElectroquímicoA novel electrochemical bioplatform for Pru du 6 determination, one of the most abundant and allergenic almond proteins, was developed to detect traces of almonds in processed foods. The bioplatform combines the application of a sandwich immunoassay on the surface of magnetic beads using specific antibodies unmodified or conjugated to horseradish peroxidase as capture and detection antibodies. The resulting magnetic bioconjugates are trapped on the surface of disposable carbon electrodes, and amperometry in the presence of hydrogen peroxide and hydroquinone is employed to measure the change in the cathodic current, which is proportional to Pru du 6 concentration. The developed bioplatform exhibited good selectivity and sensitivity, offering a detection limit of 0.12 ng/mL of Pru du 6. It could determine Pru du 6 in raw dough and baked cookies incurred with 0.2 µg/ g of almond protein with acceptable precision, providing results comparable to those obtained using an ELISA technique.ElsevierUniversidad Complutense de Madrid20232023-01-0120232023-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/114295reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1142952026-06-02T12:44:21Z
dc.title.none.fl_str_mv First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6
title First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6
spellingShingle First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6
Civera, Alba
543
Electrochemical immunoplatform
Almond
Pru du 6
Incurred cookies
Biosensor
Química analítica (Química)
2301.04 Análisis Electroquímico
title_short First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6
title_full First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6
title_fullStr First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6
title_full_unstemmed First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6
title_sort First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6
dc.creator.none.fl_str_mv Civera, Alba
Tvorynska, Sofiia
Gamella Carballo, María
Torrente Rodríguez, Rebeca Magnolia
Pedrero Muñoz, María
Galan-Malo, Patricia
Mata, Luis
Sanchez, Lourdes
Barek, Jiri
Pingarrón Carrazón, José Manuel
Campuzano Ruiz, Susana
Perez, Maria D.
author Civera, Alba
author_facet Civera, Alba
Tvorynska, Sofiia
Gamella Carballo, María
Torrente Rodríguez, Rebeca Magnolia
Pedrero Muñoz, María
Galan-Malo, Patricia
Mata, Luis
Sanchez, Lourdes
Barek, Jiri
Pingarrón Carrazón, José Manuel
Campuzano Ruiz, Susana
Perez, Maria D.
author_role author
author2 Tvorynska, Sofiia
Gamella Carballo, María
Torrente Rodríguez, Rebeca Magnolia
Pedrero Muñoz, María
Galan-Malo, Patricia
Mata, Luis
Sanchez, Lourdes
Barek, Jiri
Pingarrón Carrazón, José Manuel
Campuzano Ruiz, Susana
Perez, Maria D.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Electrochemical immunoplatform
Almond
Pru du 6
Incurred cookies
Biosensor
Química analítica (Química)
2301.04 Análisis Electroquímico
topic 543
Electrochemical immunoplatform
Almond
Pru du 6
Incurred cookies
Biosensor
Química analítica (Química)
2301.04 Análisis Electroquímico
description A novel electrochemical bioplatform for Pru du 6 determination, one of the most abundant and allergenic almond proteins, was developed to detect traces of almonds in processed foods. The bioplatform combines the application of a sandwich immunoassay on the surface of magnetic beads using specific antibodies unmodified or conjugated to horseradish peroxidase as capture and detection antibodies. The resulting magnetic bioconjugates are trapped on the surface of disposable carbon electrodes, and amperometry in the presence of hydrogen peroxide and hydroquinone is employed to measure the change in the cathodic current, which is proportional to Pru du 6 concentration. The developed bioplatform exhibited good selectivity and sensitivity, offering a detection limit of 0.12 ng/mL of Pru du 6. It could determine Pru du 6 in raw dough and baked cookies incurred with 0.2 µg/ g of almond protein with acceptable precision, providing results comparable to those obtained using an ELISA technique.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/114295
url https://hdl.handle.net/20.500.14352/114295
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409973040054272
score 15,812429

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