Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects

Melatonin is involved in various functions such as the timing of circadian rhythms, energy metabolism, and body mass gain in experimental animals. However, its effects on adipose tissue lipid metabolism are still unclear. This study analyzes the effects of melatonin on the relative gene expression o...

Descripción completa

Detalles Bibliográficos
Autores: Cano Barquilla, María Pilar, Jiménez Ortega, Vanesa, Fernández Mateos, María Del Pilar, Virto Ruiz, Leire, Maldonado Bautista, Estela, Pérez De Miguelsanz, María Juliana, Esquifino, Ana I.
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/118344
Acceso en línea:https://hdl.handle.net/20.500.14352/118344
Access Level:acceso abierto
Palabra clave:577.2
Obesity
Melatonin
Lipolysis
Mesenteric adipose tissue
Rats
Ciencias Biomédicas
2302.21 Biología Molecular
id ES_6817ec7ea3b291ebc95c917efdad5a4e
oai_identifier_str oai:docta.ucm.es:20.500.14352/118344
network_acronym_str ES
network_name_str España
repository_id_str
spelling Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin EffectsCano Barquilla, María PilarJiménez Ortega, VanesaFernández Mateos, María Del PilarVirto Ruiz, LeireMaldonado Bautista, EstelaPérez De Miguelsanz, María JulianaEsquifino, Ana I.577.2ObesityMelatoninLipolysisMesenteric adipose tissueRatsCiencias Biomédicas2302.21 Biología MolecularMelatonin is involved in various functions such as the timing of circadian rhythms, energy metabolism, and body mass gain in experimental animals. However, its effects on adipose tissue lipid metabolism are still unclear. This study analyzes the effects of melatonin on the relative gene expression of lipolytic proteins in rat mesenteric adipose tissue and free fatty acid (FFA) and glycerol plasma levels of male Wistar rats fed a high-fat (HFD) or maintenance diet. Four experimental groups were established: control, obese, and control or obese plus 2.3 mg/kg/day of melatonin in tap water. After 11 weeks, animals were sacrificed at different times throughout a 24 h cycle, and mesenteric adipose tissue and plasma samples were collected and analyzed. Cgi58, Perilipin, and Dgat1 gene expression, as well as FFA and glycerol concentrations, showed rhythm patterns in the control group. HFD disrupted those rhythm patterns and increased FFA and glycerol concentrations during the dark photoperiod. In both melatonin-treated groups, almost all analyzed genes showed circadian patterns. Notably, melatonin significantly prevented the increase in FFA levels during the dark photoperiod in obese rats (obese group: ~1100 mM vs. obese + melatonin group: ~600 μM, similar to control levels). However, the rhythmic pattern observed in control animals was not sustained. According to our results, melatonin could regulate circadian gene transcription of mesenteric adipose tissue lipolysis proteins. The effect of melatonin on preventing elevated FFA plasma levels associated with high-fat diet intake warrants further investigation.PubMedUniversidad Complutense de Madrid20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/118344reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 Not available SAF2008-00442 EFECTO PROTECTOR DE LA MELATONINA EN LA INDUCCION DE OBESIDAD POR DIETA HIPERCALORICAopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-sa/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1183442026-06-02T12:44:21Z
dc.title.none.fl_str_mv Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects
title Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects
spellingShingle Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects
Cano Barquilla, María Pilar
577.2
Obesity
Melatonin
Lipolysis
Mesenteric adipose tissue
Rats
Ciencias Biomédicas
2302.21 Biología Molecular
title_short Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects
title_full Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects
title_fullStr Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects
title_full_unstemmed Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects
title_sort Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects
dc.creator.none.fl_str_mv Cano Barquilla, María Pilar
Jiménez Ortega, Vanesa
Fernández Mateos, María Del Pilar
Virto Ruiz, Leire
Maldonado Bautista, Estela
Pérez De Miguelsanz, María Juliana
Esquifino, Ana I.
author Cano Barquilla, María Pilar
author_facet Cano Barquilla, María Pilar
Jiménez Ortega, Vanesa
Fernández Mateos, María Del Pilar
Virto Ruiz, Leire
Maldonado Bautista, Estela
Pérez De Miguelsanz, María Juliana
Esquifino, Ana I.
author_role author
author2 Jiménez Ortega, Vanesa
Fernández Mateos, María Del Pilar
Virto Ruiz, Leire
Maldonado Bautista, Estela
Pérez De Miguelsanz, María Juliana
Esquifino, Ana I.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.2
Obesity
Melatonin
Lipolysis
Mesenteric adipose tissue
Rats
Ciencias Biomédicas
2302.21 Biología Molecular
topic 577.2
Obesity
Melatonin
Lipolysis
Mesenteric adipose tissue
Rats
Ciencias Biomédicas
2302.21 Biología Molecular
description Melatonin is involved in various functions such as the timing of circadian rhythms, energy metabolism, and body mass gain in experimental animals. However, its effects on adipose tissue lipid metabolism are still unclear. This study analyzes the effects of melatonin on the relative gene expression of lipolytic proteins in rat mesenteric adipose tissue and free fatty acid (FFA) and glycerol plasma levels of male Wistar rats fed a high-fat (HFD) or maintenance diet. Four experimental groups were established: control, obese, and control or obese plus 2.3 mg/kg/day of melatonin in tap water. After 11 weeks, animals were sacrificed at different times throughout a 24 h cycle, and mesenteric adipose tissue and plasma samples were collected and analyzed. Cgi58, Perilipin, and Dgat1 gene expression, as well as FFA and glycerol concentrations, showed rhythm patterns in the control group. HFD disrupted those rhythm patterns and increased FFA and glycerol concentrations during the dark photoperiod. In both melatonin-treated groups, almost all analyzed genes showed circadian patterns. Notably, melatonin significantly prevented the increase in FFA levels during the dark photoperiod in obese rats (obese group: ~1100 mM vs. obese + melatonin group: ~600 μM, similar to control levels). However, the rhythmic pattern observed in control animals was not sustained. According to our results, melatonin could regulate circadian gene transcription of mesenteric adipose tissue lipolysis proteins. The effect of melatonin on preventing elevated FFA plasma levels associated with high-fat diet intake warrants further investigation.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/118344
url https://hdl.handle.net/20.500.14352/118344
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 Not available SAF2008-00442 EFECTO PROTECTOR DE LA MELATONINA EN LA INDUCCION DE OBESIDAD POR DIETA HIPERCALORICA
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PubMed
publisher.none.fl_str_mv PubMed
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409936369254400
score 15,812429

Similares en LA Referencia