Per- and Polyfluoroalkyl Substances in Urine Samples from Eight-Year-Old Children Living in Northwest Spain

Per- and polyfluoroalkyl substances (PFAS) are synthetics prized for their chemical stability and functionality. Legacy PFAS such as perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) have been phased out due to their persistence and toxicity. This study assessed exposure to both...

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Detalhes bibliográficos
Autores: Bautista, Arianna, Fernández-Tardón, Guillermo, Rodríguez-Suárez, Marta M., Tardón, Adonina, Bravo, Natalia, Garí, Mercè, Grimalt, Joan O., Llorca, Marta, Farré, Marinella
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/425214
Acesso em linha:http://hdl.handle.net/10261/425214
https://api.elsevier.com/content/abstract/scopus_id/105032811178
Access Level:acceso abierto
Palavra-chave:Urine
Children cohort
Human biomonitoring
Human matrices
Mass spectrometry
Per- and polyfluoroalkyl substances
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/12
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
Make cities and human settlements inclusive, safe, resilient and sustainable
Ensure sustainable consumption and production patterns
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oai_identifier_str oai:digital.csic.es:10261/425214
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Per- and Polyfluoroalkyl Substances in Urine Samples from Eight-Year-Old Children Living in Northwest Spain
title Per- and Polyfluoroalkyl Substances in Urine Samples from Eight-Year-Old Children Living in Northwest Spain
spellingShingle Per- and Polyfluoroalkyl Substances in Urine Samples from Eight-Year-Old Children Living in Northwest Spain
Bautista, Arianna
Urine
Children cohort
Human biomonitoring
Human matrices
Mass spectrometry
Per- and polyfluoroalkyl substances
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/12
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
Make cities and human settlements inclusive, safe, resilient and sustainable
Ensure sustainable consumption and production patterns
title_short Per- and Polyfluoroalkyl Substances in Urine Samples from Eight-Year-Old Children Living in Northwest Spain
title_full Per- and Polyfluoroalkyl Substances in Urine Samples from Eight-Year-Old Children Living in Northwest Spain
title_fullStr Per- and Polyfluoroalkyl Substances in Urine Samples from Eight-Year-Old Children Living in Northwest Spain
title_full_unstemmed Per- and Polyfluoroalkyl Substances in Urine Samples from Eight-Year-Old Children Living in Northwest Spain
title_sort Per- and Polyfluoroalkyl Substances in Urine Samples from Eight-Year-Old Children Living in Northwest Spain
dc.creator.none.fl_str_mv Bautista, Arianna
Fernández-Tardón, Guillermo
Rodríguez-Suárez, Marta M.
Tardón, Adonina
Bravo, Natalia
Garí, Mercè
Grimalt, Joan O.
Llorca, Marta
Farré, Marinella
author Bautista, Arianna
author_facet Bautista, Arianna
Fernández-Tardón, Guillermo
Rodríguez-Suárez, Marta M.
Tardón, Adonina
Bravo, Natalia
Garí, Mercè
Grimalt, Joan O.
Llorca, Marta
Farré, Marinella
author_role author
author2 Fernández-Tardón, Guillermo
Rodríguez-Suárez, Marta M.
Tardón, Adonina
Bravo, Natalia
Garí, Mercè
Grimalt, Joan O.
Llorca, Marta
Farré, Marinella
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Generalitat de Catalunya
Fernández-Tardón, Guillermo [0000-0002-7680-158X]
Rodríguez-Suárez, Marta M. [0000-0003-0835-5837]
Tardón, Adonina [0000-0001-5150-1209]
Garí, Mercè [0000-0001-6903-0732]
Grimalt, Joan O. [0000-0002-7391-5768]
Llorca, Marta [0000-0002-6065-2129]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Urine
Children cohort
Human biomonitoring
Human matrices
Mass spectrometry
Per- and polyfluoroalkyl substances
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/12
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
Make cities and human settlements inclusive, safe, resilient and sustainable
Ensure sustainable consumption and production patterns
topic Urine
Children cohort
Human biomonitoring
Human matrices
Mass spectrometry
Per- and polyfluoroalkyl substances
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/12
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
Make cities and human settlements inclusive, safe, resilient and sustainable
Ensure sustainable consumption and production patterns
description Per- and polyfluoroalkyl substances (PFAS) are synthetics prized for their chemical stability and functionality. Legacy PFAS such as perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) have been phased out due to their persistence and toxicity. This study assessed exposure to both legacy and emerging PFAS in 281 urine samples from 8-year-old children participating in the (Infancia y Medio Ambiente) INMA Asturias birth cohort (northwest Spain), a region with a strong industrial background. Dietary and household information was collected via questionnaires, and urine samples were analysed using ultra-high-performance liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) with full-scan acquisition in independent all-ion fragmentation mode. A suspected screening approach was applied to discover previously unreported PFAS and expand the detectable chemical profile, complemented by targeted analysis of 29 compounds selected for their persistence and regulatory relevance. Among them, 17 compounds were confirmed and quantified. The combined targeted and suspect-screening approach also identified novel PFAS, including fluorotelomer carboxylic acids, demonstrating the value of LC-HRMS for detecting unregulated compounds. Emerging PFAS showed the highest detection frequencies and concentrations: trifluoroacetic acid (TFA) and hexafluoropropylene oxide dimer acid (HFPO-DA, GenX) were detected in 63% and 27% of samples, respectively, with GenX reaching 10.1 ng/mL, whereas PFOA and PFOS were detected less frequently (8.5% and 3.2%) and at concentrations below 1 ng/mL, highlighting the need for epidemiological studies to achieve comprehensive PFAS exposure assessments. Associations with dietary habit exposure estimates point to dairy, protein-rich foods, vegetables, and drinking water as the main contributors.
publishDate 2026
dc.date.none.fl_str_mv 2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/425214
https://api.elsevier.com/content/abstract/scopus_id/105032811178
url http://hdl.handle.net/10261/425214
https://api.elsevier.com/content/abstract/scopus_id/105032811178
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/HE/101057014
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/molecules31050900
https://doi.org/10.3390/molecules31050900

dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Per- and Polyfluoroalkyl Substances in Urine Samples from Eight-Year-Old Children Living in Northwest SpainBautista, AriannaFernández-Tardón, GuillermoRodríguez-Suárez, Marta M.Tardón, AdoninaBravo, NataliaGarí, MercèGrimalt, Joan O.Llorca, MartaFarré, MarinellaUrineChildren cohortHuman biomonitoringHuman matricesMass spectrometryPer- and polyfluoroalkyl substanceshttp://metadata.un.org/sdg/11http://metadata.un.org/sdg/12http://metadata.un.org/sdg/3http://metadata.un.org/sdg/9Ensure healthy lives and promote well-being for all at all agesBuild resilient infrastructure, promote inclusive and sustainable industrialization and foster innovationMake cities and human settlements inclusive, safe, resilient and sustainableEnsure sustainable consumption and production patternsPer- and polyfluoroalkyl substances (PFAS) are synthetics prized for their chemical stability and functionality. Legacy PFAS such as perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) have been phased out due to their persistence and toxicity. This study assessed exposure to both legacy and emerging PFAS in 281 urine samples from 8-year-old children participating in the (Infancia y Medio Ambiente) INMA Asturias birth cohort (northwest Spain), a region with a strong industrial background. Dietary and household information was collected via questionnaires, and urine samples were analysed using ultra-high-performance liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) with full-scan acquisition in independent all-ion fragmentation mode. A suspected screening approach was applied to discover previously unreported PFAS and expand the detectable chemical profile, complemented by targeted analysis of 29 compounds selected for their persistence and regulatory relevance. Among them, 17 compounds were confirmed and quantified. The combined targeted and suspect-screening approach also identified novel PFAS, including fluorotelomer carboxylic acids, demonstrating the value of LC-HRMS for detecting unregulated compounds. Emerging PFAS showed the highest detection frequencies and concentrations: trifluoroacetic acid (TFA) and hexafluoropropylene oxide dimer acid (HFPO-DA, GenX) were detected in 63% and 27% of samples, respectively, with GenX reaching 10.1 ng/mL, whereas PFOA and PFOS were detected less frequently (8.5% and 3.2%) and at concentrations below 1 ng/mL, highlighting the need for epidemiological studies to achieve comprehensive PFAS exposure assessments. Associations with dietary habit exposure estimates point to dairy, protein-rich foods, vegetables, and drinking water as the main contributors.This work was carried out in the framework of the European Partnership for the Assessment of Risks from Chemicals (PARC; Grant Agreement No. 101057014). It also received funding from the Horizon European Projects NEMESIS (HLTH-2023-ENVHLTH-02-03-101137405), ENVESOME (HLTH-2024-ENVHLTH-02-06-two-stage-101157269), and the Generalitat de Catalunya through the Consolidated Research Groups 2021 SGR 01150 and 2021 SGR 00986. The views and opinions expressed are, however, those of the author(s) only, and do not necessarily reflect those of the European Union or the Health and Digital Executive Agency. Neither the European Union nor the granting authority can be held responsible for such views and opinions.Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionGeneralitat de CatalunyaFernández-Tardón, Guillermo [0000-0002-7680-158X]Rodríguez-Suárez, Marta M. [0000-0003-0835-5837]Tardón, Adonina [0000-0001-5150-1209]Garí, Mercè [0000-0001-6903-0732]Grimalt, Joan O. [0000-0002-7391-5768]Llorca, Marta [0000-0002-6065-2129]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262026info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/425214https://api.elsevier.com/content/abstract/scopus_id/105032811178reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/HE/101057014The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/molecules31050900https://doi.org/10.3390/molecules31050900Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4252142026-05-22T06:33:51Z
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