Genomic characterization of liver metastases from colorectal cancer patients

[EN]Metastatic dissemination is the most frequent cause of death of sporadic colorectal cancer (sCRC) patients. Genomic abnormalities which are potentially characteristic of such advanced stages of the disease are complex and so far, they have been poorly described and only partially understood. We...

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Detalhes bibliográficos
Autores: Sayagués Manzano, José María, Corchete Sánchez, Luis Antonio, Gutiérrez Troncoso, María Laura, Alonso Sarasquete, María Eugenia, Abad Hernández, María Mar, Bengoechea Miranda, Oscar, Fermiñán, Encarna, Anduaga, María Fernanda, Carmen Martínez, Sofía del, Iglesias Iglesias, Manuel José, Esteban Velasco, María Carmen, Angoso Clavijo, María, Alcázar Montero, José Antonio, García García, Jacinto, Orfao de Matos Correia e Vale, José Alberto, Muñoz‐Bellvis, Luís, Gutiérrez, María Laura, Sarasquete, Maria Eugenia, del Mar Abad, María, Bengoechea, Oscar, del Carmen, Sofia, Iglesias, Manuel, Esteban, Carmen, Angoso, María, García, Jacinto, Muñoz-Bellvis, Luís
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/160917
Acesso em linha:http://hdl.handle.net/10366/160917
Access Level:acceso abierto
Palavra-chave:GEP
Colorectal cancer
Neoplasm Metastasis
Colorectal Neoplasms
3201.01 Oncología
metástasis neoplásica
neoplasias colorrectales
Descrição
Resumo:[EN]Metastatic dissemination is the most frequent cause of death of sporadic colorectal cancer (sCRC) patients. Genomic abnormalities which are potentially characteristic of such advanced stages of the disease are complex and so far, they have been poorly described and only partially understood. We evaluated the molecular heterogeneity of sCRC tumors based on simultaneous assessment of the overall GEP of both coding mRNA and non-coding RNA genes in primary sCRC tumor samples from 23 consecutive patients and their paired liver metastases. Liver metastases from the sCRC patients analyzed, systematically showed deregulated transcripts of those genes identified as also deregulated in their paired primary colorectal carcinomas. However, some transcripts were found to be specifically deregulated in liver metastases (vs. non-tumoral colorectal tissues) while expressed at normal levels in their primary tumors, reflecting either an increased genomic instability of metastatic cells or theiradaption to the liver microenvironment. Newly deregulated metastatic transcripts included overexpression of APOA1, HRG, UGT2B4, RBP4 and ADH4 mRNAS and the miR-3180-3p, miR-3197, miR-3178, miR-4793 and miR-4440 miRNAs, together with decreased expression of the IGKV1-39, IGKC, IGKV1-27, FABP4 and MYLK mRNAS and the miR-363, miR-1, miR-143, miR-27b and miR-28-5p miRNAs. Canonical pathways found to be specifically deregulated in liver metastatic samples included multiple genes related with intercellular adhesion and the metastatic processes (e.g., IGF1R, PIK3CA, PTEN and EGFR), endocytosis (e.g., the PDGFRA, SMAD2, ERBB3, PML and FGFR2), and the cell cycle (e.g., SMAD2, CCND2, E2F5 and MYC). Our results also highlighted the activation of genes associated with the TGFβ signaling pathway, -e.g. RHOA, SMAD2, SMAD4, SMAD5, SMAD6, BMPR1A, SMAD7 and MYC-, which thereby emerge as candidate genes to play an important role in CRC tumor metastasis.

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