Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance
[Background]: Plant responses to a wide range of stresses are known to be regulated by epigenetic mechanisms. Pathogen-related investigations, particularly against RNA viruses, are however scarce. It has been demonstrated that Arabidopsis thaliana plants defective in some members of the RNA-directed...
| Autores: | , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/360579 |
| Acesso em linha: | http://hdl.handle.net/10261/360579 |
| Access Level: | acceso abierto |
| Palavra-chave: | Biotic stress Defense priming Epigenetics Histone modifications Induced resistance Potyvirus RNA-directed DNA methylation |
| id |
ES_664ccbe0f4e01b53a5cb476d70330464 |
|---|---|
| oai_identifier_str |
oai:digital.csic.es:10261/360579 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| dc.title.none.fl_str_mv |
Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance |
| title |
Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance |
| spellingShingle |
Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance Corrêa, Régis L. Biotic stress Defense priming Epigenetics Histone modifications Induced resistance Potyvirus RNA-directed DNA methylation |
| title_short |
Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance |
| title_full |
Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance |
| title_fullStr |
Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance |
| title_full_unstemmed |
Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance |
| title_sort |
Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance |
| dc.creator.none.fl_str_mv |
Corrêa, Régis L. Kutnjak, Denis Ambrós, Silvia Bustos, Mónica Elena, Santiago F. |
| author |
Corrêa, Régis L. |
| author_facet |
Corrêa, Régis L. Kutnjak, Denis Ambrós, Silvia Bustos, Mónica Elena, Santiago F. |
| author_role |
author |
| author2 |
Kutnjak, Denis Ambrós, Silvia Bustos, Mónica Elena, Santiago F. |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Conferencia de Rectores de las Universidades Españolas Consejo Superior de Investigaciones Científicas (España) Generalitat Valenciana Agencia Estatal de Investigación (España) Ministerio de Ciencia e Innovación (España) Ministerio de Ciencia, Innovación y Universidades (España) European Commission Slovenian Research Agency Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Biotic stress Defense priming Epigenetics Histone modifications Induced resistance Potyvirus RNA-directed DNA methylation |
| topic |
Biotic stress Defense priming Epigenetics Histone modifications Induced resistance Potyvirus RNA-directed DNA methylation |
| description |
[Background]: Plant responses to a wide range of stresses are known to be regulated by epigenetic mechanisms. Pathogen-related investigations, particularly against RNA viruses, are however scarce. It has been demonstrated that Arabidopsis thaliana plants defective in some members of the RNA-directed DNA methylation (RdDM) or histone modification pathways presented differential susceptibility to the turnip mosaic virus. In order to identify genes directly targeted by the RdDM-related RNA Polymerase V (POLV) complex and the histone demethylase protein JUMONJI14 (JMJ14) during infection, the transcriptomes of infected mutant and control plants were obtained and integrated with available chromatin occupancy data for various epigenetic proteins and marks. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024 2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/360579 |
| url |
http://hdl.handle.net/10261/360579 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136912NB-I00 The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1186/s12870-024-04866-3 https://doi.org/10.1186/s12870-024-04866-3 Sí |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
BioMed Central |
| publisher.none.fl_str_mv |
BioMed Central |
| dc.source.none.fl_str_mv |
reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| collection |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869409805792182272 |
| spelling |
Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistanceCorrêa, Régis L.Kutnjak, DenisAmbrós, SilviaBustos, MónicaElena, Santiago F.Biotic stressDefense primingEpigeneticsHistone modificationsInduced resistancePotyvirusRNA-directed DNA methylation[Background]: Plant responses to a wide range of stresses are known to be regulated by epigenetic mechanisms. Pathogen-related investigations, particularly against RNA viruses, are however scarce. It has been demonstrated that Arabidopsis thaliana plants defective in some members of the RNA-directed DNA methylation (RdDM) or histone modification pathways presented differential susceptibility to the turnip mosaic virus. In order to identify genes directly targeted by the RdDM-related RNA Polymerase V (POLV) complex and the histone demethylase protein JUMONJI14 (JMJ14) during infection, the transcriptomes of infected mutant and control plants were obtained and integrated with available chromatin occupancy data for various epigenetic proteins and marks.[Results]: A comprehensive list of virus-responsive gene candidates to be regulated by the two proteins was obtained. Twelve genes were selected for further characterization, confirming their dynamic regulation during the course of infection. Several epigenetic marks on their promoter sequences were found using in silico data, raising confidence that the identified genes are actually regulated by epigenetic mechanisms. The altered expression of six of these genes in mutants of the methyltransferase gene CURLY LEAF and the histone deacetylase gene HISTONE DEACETYLASE 19 suggests that some virus-responsive genes may be regulated by multiple coordinated epigenetic complexes. A temporally separated multiple plant virus infection experiment in which plants were transiently infected with one virus and then infected by a second one was designed to investigate the possible roles of the identified POLV- and JMJ14-regulated genes in wild-type (WT) plants. Plants that had previously been stimulated with viruses were found to be more resistant to subsequent virus challenge than control plants. Several POLV- and JMJ14-regulated genes were found to be regulated in virus induced resistance in WT plants, with some of them poisoned to be expressed in early infection stages.[Conclusions]: A set of confident candidate genes directly regulated by the POLV and JMJ14 proteins during virus infection was identified, with indications that some of them may be regulated by multiple epigenetic modules. A subset of these genes may also play a role in the tolerance of WT plants to repeated, intermittent virus infections.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature.This work was supported by the Generalitat Valenciana grants CIDEGENT/2021/030 (RLC) and CIPROM/2022/59 (SFE), by grant PID2022-136912NB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF a way of making Europe” (SFE), by the FAPERJ grant 14/2019 (RLC) and by the Slovenian Research and Innovation Agency (ARIS) core and project financing grants P4-0407 and Z1-9158 (DK).Peer reviewedBioMed CentralConferencia de Rectores de las Universidades EspañolasConsejo Superior de Investigaciones Científicas (España)Generalitat ValencianaAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionSlovenian Research AgencyFundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/360579reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136912NB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1186/s12870-024-04866-3https://doi.org/10.1186/s12870-024-04866-3Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3605792026-05-22T06:33:51Z |
| score |
15,812429 |