A fractal nature for polymerized laminin

Polylaminin (polyLM) is a non-covalent acid-induced nano- and micro-structured polymer of the protein laminin displaying distinguished biological properties. Polylaminin stimulates neuritogenesis beyond the levels achieved by ordinary laminin and has been shown to promote axonal regeneration in anim...

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Autores: Hochman Méndez, Carlos, Cantini ., Marco, Salmerón Sánchez, Manuel, Coelho-Sampaio, Tatiana, Moratal, David|||0000-0002-2825-3646
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/59795
Acceso en línea:https://riunet.upv.es/handle/10251/59795
Access Level:acceso abierto
Palabra clave:Polylaminin (polyLM)
Confocal fluorescence microscopy (CFM)
Scanning electron microscopy (SEM)
Atomic Force Microscopy (AFM)
FISICA APLICADA
TECNOLOGIA ELECTRONICA
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spelling A fractal nature for polymerized lamininHochman Méndez, CarlosCantini ., MarcoSalmerón Sánchez, ManuelCoelho-Sampaio, TatianaMoratal, David|||0000-0002-2825-3646Polylaminin (polyLM)Confocal fluorescence microscopy (CFM)Scanning electron microscopy (SEM)Atomic Force Microscopy (AFM)FISICA APLICADATECNOLOGIA ELECTRONICAPolylaminin (polyLM) is a non-covalent acid-induced nano- and micro-structured polymer of the protein laminin displaying distinguished biological properties. Polylaminin stimulates neuritogenesis beyond the levels achieved by ordinary laminin and has been shown to promote axonal regeneration in animal models of spinal cord injury. Here we used confocal fluorescence microscopy (CFM), scanning electron microscopy (SEM) and atomic force microscopy (AFM) to characterize its three-dimensional structure. Renderization of confocal optical slices of immunostained polyLM revealed the aspect of a loose flocculated meshwork, which was homogeneously stained by the antibody. On the other hand, an ordinary matrix obtained upon adsorption of laminin in neutral pH (LM) was constituted of bulky protein aggregates whose interior was not accessible to the same anti-laminin antibody. SEM and AFM analyses revealed that the seed unit of polyLM was a flat polygon formed in solution whereas the seed structure of LM was highly heterogeneous, intercalating rod-like, spherical and thin spread lamellar deposits. As polyLM was visualized at progressively increasing magnifications, we observed that the morphology of the polymer was alike independently of the magnification used for the observation. A search for the Hausdorff dimension in images of the two matrices showed that polyLM, but not LM, presented fractal dimensions of 1.55, 1.62 and 1.70 after 1, 8 and 12 hours of adsorption, respectively. Data in the present work suggest that the intrinsic fractal nature of polymerized laminin can be the structural basis for the fractal-like organization of basement membranes in the neurogenic niches of the central nervous system.This work was supported by a grant from the Brazilian National Research Council (CNPq; 476772/2008-7) to TCS. MSS acknowledges support from the European Research Council through ERC - 306990. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of ScienceDepartamento de Ingeniería ElectrónicaCentro de Biomateriales e Ingeniería TisularEscuela Técnica Superior de Ingeniería IndustrialConselho Nacional de Desenvolvimento Científico e Tecnológico, BrasilEuropean CommissionRepositorio Institucional de la Universitat Politècnica de València Riunet20142014-10-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/59795reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengEuropean Commission https://doi.org/10.13039/501100000780 FP7 306990 Material-driven Fibronectin Fibrillogenesis to Engineer Synergistic Growth Factor MicroenvironmentsConselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil https://doi.org/10.13039/501100003593 476772%2F2008-7open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/597952026-06-13T07:49:27Z
dc.title.none.fl_str_mv A fractal nature for polymerized laminin
title A fractal nature for polymerized laminin
spellingShingle A fractal nature for polymerized laminin
Hochman Méndez, Carlos
Polylaminin (polyLM)
Confocal fluorescence microscopy (CFM)
Scanning electron microscopy (SEM)
Atomic Force Microscopy (AFM)
FISICA APLICADA
TECNOLOGIA ELECTRONICA
title_short A fractal nature for polymerized laminin
title_full A fractal nature for polymerized laminin
title_fullStr A fractal nature for polymerized laminin
title_full_unstemmed A fractal nature for polymerized laminin
title_sort A fractal nature for polymerized laminin
dc.creator.none.fl_str_mv Hochman Méndez, Carlos
Cantini ., Marco
Salmerón Sánchez, Manuel
Coelho-Sampaio, Tatiana
Moratal, David|||0000-0002-2825-3646
author Hochman Méndez, Carlos
author_facet Hochman Méndez, Carlos
Cantini ., Marco
Salmerón Sánchez, Manuel
Coelho-Sampaio, Tatiana
Moratal, David|||0000-0002-2825-3646
author_role author
author2 Cantini ., Marco
Salmerón Sánchez, Manuel
Coelho-Sampaio, Tatiana
Moratal, David|||0000-0002-2825-3646
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Ingeniería Electrónica
Centro de Biomateriales e Ingeniería Tisular
Escuela Técnica Superior de Ingeniería Industrial
Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil
European Commission
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Polylaminin (polyLM)
Confocal fluorescence microscopy (CFM)
Scanning electron microscopy (SEM)
Atomic Force Microscopy (AFM)
FISICA APLICADA
TECNOLOGIA ELECTRONICA
topic Polylaminin (polyLM)
Confocal fluorescence microscopy (CFM)
Scanning electron microscopy (SEM)
Atomic Force Microscopy (AFM)
FISICA APLICADA
TECNOLOGIA ELECTRONICA
description Polylaminin (polyLM) is a non-covalent acid-induced nano- and micro-structured polymer of the protein laminin displaying distinguished biological properties. Polylaminin stimulates neuritogenesis beyond the levels achieved by ordinary laminin and has been shown to promote axonal regeneration in animal models of spinal cord injury. Here we used confocal fluorescence microscopy (CFM), scanning electron microscopy (SEM) and atomic force microscopy (AFM) to characterize its three-dimensional structure. Renderization of confocal optical slices of immunostained polyLM revealed the aspect of a loose flocculated meshwork, which was homogeneously stained by the antibody. On the other hand, an ordinary matrix obtained upon adsorption of laminin in neutral pH (LM) was constituted of bulky protein aggregates whose interior was not accessible to the same anti-laminin antibody. SEM and AFM analyses revealed that the seed unit of polyLM was a flat polygon formed in solution whereas the seed structure of LM was highly heterogeneous, intercalating rod-like, spherical and thin spread lamellar deposits. As polyLM was visualized at progressively increasing magnifications, we observed that the morphology of the polymer was alike independently of the magnification used for the observation. A search for the Hausdorff dimension in images of the two matrices showed that polyLM, but not LM, presented fractal dimensions of 1.55, 1.62 and 1.70 after 1, 8 and 12 hours of adsorption, respectively. Data in the present work suggest that the intrinsic fractal nature of polymerized laminin can be the structural basis for the fractal-like organization of basement membranes in the neurogenic niches of the central nervous system.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-10-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/59795
url https://riunet.upv.es/handle/10251/59795
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 FP7 306990 Material-driven Fibronectin Fibrillogenesis to Engineer Synergistic Growth Factor Microenvironments
Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil https://doi.org/10.13039/501100003593 476772%2F2008-7
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300724

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