Preventing Mislabeling: A Comparative Chromatographic Analysis for Classifying Medical and Industrial Cannabis
[EN] Gas chromatography (GC) techniques for analyzing and determining the cannabinoid profile in cannabis (Cannabis sativa L.) are widely used in standard laboratories; however, these methods may mislabel the profile when used under rapid conditions. Our study aimed to highlight this problem and opt...
| Authors: | , , , , , , , , , , |
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| Format: | article |
| Publication Date: | 2023 |
| Country: | España |
| Institution: | Universitat Politècnica de València (UPV) |
| Repository: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Language: | English |
| OAI Identifier: | oai:riunet.upv.es:10251/202812 |
| Online Access: | https://riunet.upv.es/handle/10251/202812 |
| Access Level: | Open access |
| Keyword: | Cannabinoids Validation Gas chromatography (GC) HPLC Overlapping 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades 08.- Fomentar el crecimiento económico sostenido, inclusivo y sostenible, el empleo pleno y productivo, y el trabajo decente para todos 16.- Promover sociedades pacíficas e inclusivas para el desarrollo sostenible, facilitar acceso a la justicia para todos y crear instituciones eficaces, responsables e inclusivas a todos los niveles 17.- Fortalecer los medios de ejecución y reavivar la alianza mundial para el desarrollo sostenible |
| Summary: | [EN] Gas chromatography (GC) techniques for analyzing and determining the cannabinoid profile in cannabis (Cannabis sativa L.) are widely used in standard laboratories; however, these methods may mislabel the profile when used under rapid conditions. Our study aimed to highlight this problem and optimize GC column conditions and mass spectrometry (MS) parameters to accurately identify cannabinoids in both standards and forensic samples. The method was validated for linearity, selectivity, and precision. It was observed that when tetrahydrocannabinol (¿9-THC) and cannabidiolic acid (CBD-A) were examined using rapid GC conditions, the resulting derivatives generated identical retention times. Wider chromatographic conditions were applied. The linear range for each compound ranged from 0.02 ¿g/mL to 37.50 ¿g/mL. The R2 values ranged from 0.996 to 0.999. The LOQ values ranged from 0.33 ¿g/mL to 5.83 ¿g/mL, and the LOD values ranged from 0.11 ¿g/mL to 1.92 ¿g/mL. The precision values ranged from 0.20% to 8.10% RSD. In addition, forensic samples were analyzed using liquid chromatography (HPLC-DAD) in an interlaboratory comparison test, with higher CBD and THC content than GC¿MS determination (p < 0.05) in samples. Overall, this study highlights the importance of optimizing GC techniques to avoid mislabeling cannabinoids in cannabis samples. |
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