Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish species

5 páginas, 4 figuras

Detalles Bibliográficos
Autores: Piñeiro, Carmen, Barros-Velázquez, Jorge, Pérez Martín, Ricardo Isaac, Gallardo, José Manuel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2000
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/57041
Acceso en línea:http://hdl.handle.net/10261/57041
Access Level:acceso abierto
Palabra clave:Gadoid fish species identification
Enzyme detection
Isoelectric focusing
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spelling Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish speciesPiñeiro, CarmenBarros-Velázquez, JorgePérez Martín, Ricardo IsaacGallardo, José ManuelGadoid fish species identificationEnzyme detectionIsoelectric focusing5 páginas, 4 figurasThe presence of specific enzymes in the sarcoplasmic protein fraction of eight gadoid fish species has been investigated following isoelectric focusing (IEF) in the 3.5–9.5 pH range. Lactate dehydrogenase (LDH) and glycerol-3-phosphate-dehydrogenase (G-3-PD) allowed the differentiation of most of the gadoids tested, even in the case of closely related species belonging to the genus Merluccius. Investigation of arginine kinase and creatine kinase afforded non-specific enzyme patterns in the species tested. Adenylate kinase and malate dehydrogenase did not give specific or reproducible enzyme patterns. Analysis of LDH and G-3-PD activities in commercial IEF gels was shown to be a reliable and reproducible technique for distinguishing closely related Merluccius spp. and other gadoid fish speciesThe authors thank the financial support of CICYT (Project ALI95-0053)Peer reviewedElsevierComisión Interministerial de Ciencia y Tecnología, CICYT (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201220122000info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/57041reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/S0308-8146(00)00042-XSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/570412026-05-22T06:33:51Z
dc.title.none.fl_str_mv Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish species
title Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish species
spellingShingle Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish species
Piñeiro, Carmen
Gadoid fish species identification
Enzyme detection
Isoelectric focusing
title_short Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish species
title_full Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish species
title_fullStr Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish species
title_full_unstemmed Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish species
title_sort Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish species
dc.creator.none.fl_str_mv Piñeiro, Carmen
Barros-Velázquez, Jorge
Pérez Martín, Ricardo Isaac
Gallardo, José Manuel
author Piñeiro, Carmen
author_facet Piñeiro, Carmen
Barros-Velázquez, Jorge
Pérez Martín, Ricardo Isaac
Gallardo, José Manuel
author_role author
author2 Barros-Velázquez, Jorge
Pérez Martín, Ricardo Isaac
Gallardo, José Manuel
author2_role author
author
author
dc.contributor.none.fl_str_mv Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Gadoid fish species identification
Enzyme detection
Isoelectric focusing
topic Gadoid fish species identification
Enzyme detection
Isoelectric focusing
description 5 páginas, 4 figuras
publishDate 2000
dc.date.none.fl_str_mv 2000
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/57041
url http://hdl.handle.net/10261/57041
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/S0308-8146(00)00042-X
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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