The transcriptional aftermath in two independently formed hybrids of the opportunistic pathogen Candida orthopsilosis
Interspecific hybridization can drive evolutionary adaptation to novel environments. The Saccharomycotina clade of budding yeasts includes many hybrid lineages, and hybridization has been proposed as a source for new pathogenic species. Candida orthopsilosis is an emerging opportunistic pathogen for...
| Autores: | , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universitat Pompeu Fabra |
| Repositorio: | Repositorio Digital de la UPF |
| OAI Identifier: | oai:repositori.upf.edu:10230/46780 |
| Acceso en línea: | http://hdl.handle.net/10230/46780 http://dx.doi.org/10.1128/mSphere.00282-20 |
| Access Level: | acceso abierto |
| Palabra clave: | Candida orthopsilosis Hybridization Pathogen Transcriptomics Yeasts |
| id |
ES_63ff15d366724f366a3d35cd0b3a8d62 |
|---|---|
| oai_identifier_str |
oai:repositori.upf.edu:10230/46780 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
The transcriptional aftermath in two independently formed hybrids of the opportunistic pathogen Candida orthopsilosisHovhannisyan, Hrant, 1992-Saus Martínez, EsterKsiezopolska, EwaGabaldón Estevan, Juan Antonio, 1973-Candida orthopsilosisHybridizationPathogenTranscriptomicsYeastsInterspecific hybridization can drive evolutionary adaptation to novel environments. The Saccharomycotina clade of budding yeasts includes many hybrid lineages, and hybridization has been proposed as a source for new pathogenic species. Candida orthopsilosis is an emerging opportunistic pathogen for which most clinical isolates are hybrids, each derived from one of at least four independent crosses between the same two parental lineages. To gain insight into the transcriptomic aftermath of hybridization in these pathogens, we analyzed allele-specific gene expression in two independently formed hybrid strains and in a homozygous strain representative of one parental lineage. Our results show that the effect of hybridization on overall gene expression is rather limited, affecting ∼4% of the genes studied. However, we identified a larger effect in terms of imbalanced allelic expression, affecting ∼9.5% of the heterozygous genes in the hybrids. This effect was larger in the hybrid with more extensive loss of heterozygosity, which may indicate a tendency to avoid loss of heterozygosity in these genes. Consistently, the number of shared genes with allele-specific expression in the two independently formed hybrids was higher than random expectation, suggesting selective retention. Some of the imbalanced genes have functions related to pathogenicity, including zinc transport and superoxide dismutase activities. While it remains unclear whether the observed imbalanced genes play a role in virulence, our results suggest that differences in allele-specific expression may add an additional layer of phenotypic plasticity to traits related to virulence in C. orthopsilosis hybrids.IMPORTANCE How new pathogens emerge is an important question that remains largely unanswered. Some emerging yeast pathogens are hybrids originated through the crossing of two different species, but how hybridization contributes to higher virulence is unclear. Here, we show that hybrids selectively retain gene regulation plasticity inherited from the two parents and that this plasticity affects genes involved in virulence.This work received funding from the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie grant agreement H2020-MSCA-ITN-2014-642095. T.G.’s research group also acknowledges support from the Spanish Ministry of Economy, Industry, and Competitiveness (MEIC) for the EMBL partnership, from grants “Centro de Excelencia Severo Ochoa 2013–2017” SEV-2012-0208, and BFU2015-67107 cofounded by European Regional Development Fund (ERDF), from the CERCA Program/Generalitat de Catalunya, from the Catalan Research Agency (AGAUR) SGR857, and grants from the European Union’s Horizon 2020 Research and Innovation Program under grant agreement ERC-2016-724173. T.G. also receives support from an INB grant (PT17/0009/0023 – ISCIII-SGEFI/ERDF).American Society for Microbiology202120212020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/46780http://dx.doi.org/10.1128/mSphere.00282-20reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésmSphere. 2020; 5(3):e00282-20info:eu-repo/grantAgreement/EC/H2020/642095info:eu-repo/grantAgreement/ES/1PE/BFU2015-67107info:eu-repo/grantAgreement/EC/H2020/724173Copyright © 2020 Hovhannisyan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/467802026-06-12T07:21:37Z |
| dc.title.none.fl_str_mv |
The transcriptional aftermath in two independently formed hybrids of the opportunistic pathogen Candida orthopsilosis |
| title |
The transcriptional aftermath in two independently formed hybrids of the opportunistic pathogen Candida orthopsilosis |
| spellingShingle |
The transcriptional aftermath in two independently formed hybrids of the opportunistic pathogen Candida orthopsilosis Hovhannisyan, Hrant, 1992- Candida orthopsilosis Hybridization Pathogen Transcriptomics Yeasts |
| title_short |
The transcriptional aftermath in two independently formed hybrids of the opportunistic pathogen Candida orthopsilosis |
| title_full |
The transcriptional aftermath in two independently formed hybrids of the opportunistic pathogen Candida orthopsilosis |
| title_fullStr |
The transcriptional aftermath in two independently formed hybrids of the opportunistic pathogen Candida orthopsilosis |
| title_full_unstemmed |
The transcriptional aftermath in two independently formed hybrids of the opportunistic pathogen Candida orthopsilosis |
| title_sort |
The transcriptional aftermath in two independently formed hybrids of the opportunistic pathogen Candida orthopsilosis |
| dc.creator.none.fl_str_mv |
Hovhannisyan, Hrant, 1992- Saus Martínez, Ester Ksiezopolska, Ewa Gabaldón Estevan, Juan Antonio, 1973- |
| author |
Hovhannisyan, Hrant, 1992- |
| author_facet |
Hovhannisyan, Hrant, 1992- Saus Martínez, Ester Ksiezopolska, Ewa Gabaldón Estevan, Juan Antonio, 1973- |
| author_role |
author |
| author2 |
Saus Martínez, Ester Ksiezopolska, Ewa Gabaldón Estevan, Juan Antonio, 1973- |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
Candida orthopsilosis Hybridization Pathogen Transcriptomics Yeasts |
| topic |
Candida orthopsilosis Hybridization Pathogen Transcriptomics Yeasts |
| description |
Interspecific hybridization can drive evolutionary adaptation to novel environments. The Saccharomycotina clade of budding yeasts includes many hybrid lineages, and hybridization has been proposed as a source for new pathogenic species. Candida orthopsilosis is an emerging opportunistic pathogen for which most clinical isolates are hybrids, each derived from one of at least four independent crosses between the same two parental lineages. To gain insight into the transcriptomic aftermath of hybridization in these pathogens, we analyzed allele-specific gene expression in two independently formed hybrid strains and in a homozygous strain representative of one parental lineage. Our results show that the effect of hybridization on overall gene expression is rather limited, affecting ∼4% of the genes studied. However, we identified a larger effect in terms of imbalanced allelic expression, affecting ∼9.5% of the heterozygous genes in the hybrids. This effect was larger in the hybrid with more extensive loss of heterozygosity, which may indicate a tendency to avoid loss of heterozygosity in these genes. Consistently, the number of shared genes with allele-specific expression in the two independently formed hybrids was higher than random expectation, suggesting selective retention. Some of the imbalanced genes have functions related to pathogenicity, including zinc transport and superoxide dismutase activities. While it remains unclear whether the observed imbalanced genes play a role in virulence, our results suggest that differences in allele-specific expression may add an additional layer of phenotypic plasticity to traits related to virulence in C. orthopsilosis hybrids.IMPORTANCE How new pathogens emerge is an important question that remains largely unanswered. Some emerging yeast pathogens are hybrids originated through the crossing of two different species, but how hybridization contributes to higher virulence is unclear. Here, we show that hybrids selectively retain gene regulation plasticity inherited from the two parents and that this plasticity affects genes involved in virulence. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2021 2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10230/46780 http://dx.doi.org/10.1128/mSphere.00282-20 |
| url |
http://hdl.handle.net/10230/46780 http://dx.doi.org/10.1128/mSphere.00282-20 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
mSphere. 2020; 5(3):e00282-20 info:eu-repo/grantAgreement/EC/H2020/642095 info:eu-repo/grantAgreement/ES/1PE/BFU2015-67107 info:eu-repo/grantAgreement/EC/H2020/724173 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
American Society for Microbiology |
| publisher.none.fl_str_mv |
American Society for Microbiology |
| dc.source.none.fl_str_mv |
reponame:Repositorio Digital de la UPF instname:Universitat Pompeu Fabra |
| instname_str |
Universitat Pompeu Fabra |
| reponame_str |
Repositorio Digital de la UPF |
| collection |
Repositorio Digital de la UPF |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869409613785333760 |
| score |
15,811543 |