Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer

Puig, Pere; Erill, Nadina; Terricabras, Marta; Subirana, Isaac; González-García, Judit; Asensi-Puig, Adrià; Donovan, Michael J.; Mengual Brichs, Lourdes; Agulló-Ortuño, M. Teresa; Olivan Riera, Mireia; Alcaraz Asensio, Antonio; López-Martín, José A.; Torres, Inés de; Rodríguez Peralto, José Luis; Rodríguez-Antolín, Alfredo; Morote, Juan; González-Rumayor, Víctor

Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer

Objectives: To apply a systems pathology-based approach to the quantification of nuclear Active β-catenin and human leukocyte antigen class I, and assess the biomarker involvement in a cohort of prostate tumor patients. Results: The systems pathology approach applied allows a precise quantification...

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Detalles Bibliográficos
Autores:	Puig, Pere, Erill, Nadina, Terricabras, Marta, Subirana, Isaac, González-García, Judit, Asensi-Puig, Adrià, Donovan, Michael J., Mengual Brichs, Lourdes, Agulló-Ortuño, M. Teresa, Olivan Riera, Mireia, Alcaraz Asensio, Antonio, López-Martín, José A., Torres, Inés de, Rodríguez Peralto, José Luis, Rodríguez-Antolín, Alfredo, Morote, Juan, González-Rumayor, Víctor
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2019
País:	España
Institución:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:2445/164367
Acceso en línea:	https://hdl.handle.net/2445/164367
Access Level:	acceso abierto
Palabra clave:	Marcadors bioquímics Càncer de pròstata Proteïnes Biochemical markers Prostate cancer Proteins

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spelling	Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancerPuig, PereErill, NadinaTerricabras, MartaSubirana, IsaacGonzález-García, JuditAsensi-Puig, AdriàDonovan, Michael J.Mengual Brichs, LourdesAgulló-Ortuño, M. TeresaOlivan Riera, MireiaAlcaraz Asensio, AntonioLópez-Martín, José A.Torres, Inés deRodríguez Peralto, José LuisRodríguez-Antolín, AlfredoMorote, JuanGonzález-Rumayor, VíctorMarcadors bioquímicsCàncer de pròstataProteïnesBiochemical markersProstate cancerProteinsObjectives: To apply a systems pathology-based approach to the quantification of nuclear Active β-catenin and human leukocyte antigen class I, and assess the biomarker involvement in a cohort of prostate tumor patients. Results: The systems pathology approach applied allows a precise quantification of the marker expression in the different cell compartments as well as the determination of the areas that coexpress two markers. Our data shows that the accumulation of β-catenin in the nuclear compartment is significantly decreased in the adjacent normal areas when compared to tumor of the same patients (p < 0.001). In conclusion, the application of this novel multiple immunofluorescence assay demonstrates that the upregulation of Active β-catenin is a relatively common feature of prostate tumor development, and further supports the activation of the Wnt/β-catenin pathway in prostate cancer progression.BioMed Central2020202020192020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion5 p.application/pdfhttps://hdl.handle.net/2445/164367Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1186/s13104-019-4100-zBMC Research Notes, 2019, vol. 12, p. 68https://doi.org/10.1186/s13104-019-4100-zcc-by (c) Puig, Pere et al., 2019http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1643672026-05-29T05:05:01Z
dc.title.none.fl_str_mv	Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer
title	Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer
spellingShingle	Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer Puig, Pere Marcadors bioquímics Càncer de pròstata Proteïnes Biochemical markers Prostate cancer Proteins
title_short	Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer
title_full	Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer
title_fullStr	Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer
title_full_unstemmed	Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer
title_sort	Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer
dc.creator.none.fl_str_mv	Puig, Pere Erill, Nadina Terricabras, Marta Subirana, Isaac González-García, Judit Asensi-Puig, Adrià Donovan, Michael J. Mengual Brichs, Lourdes Agulló-Ortuño, M. Teresa Olivan Riera, Mireia Alcaraz Asensio, Antonio López-Martín, José A. Torres, Inés de Rodríguez Peralto, José Luis Rodríguez-Antolín, Alfredo Morote, Juan González-Rumayor, Víctor
author	Puig, Pere
author_facet	Puig, Pere Erill, Nadina Terricabras, Marta Subirana, Isaac González-García, Judit Asensi-Puig, Adrià Donovan, Michael J. Mengual Brichs, Lourdes Agulló-Ortuño, M. Teresa Olivan Riera, Mireia Alcaraz Asensio, Antonio López-Martín, José A. Torres, Inés de Rodríguez Peralto, José Luis Rodríguez-Antolín, Alfredo Morote, Juan González-Rumayor, Víctor
author_role	author
author2	Erill, Nadina Terricabras, Marta Subirana, Isaac González-García, Judit Asensi-Puig, Adrià Donovan, Michael J. Mengual Brichs, Lourdes Agulló-Ortuño, M. Teresa Olivan Riera, Mireia Alcaraz Asensio, Antonio López-Martín, José A. Torres, Inés de Rodríguez Peralto, José Luis Rodríguez-Antolín, Alfredo Morote, Juan González-Rumayor, Víctor
author2_role	author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Marcadors bioquímics Càncer de pròstata Proteïnes Biochemical markers Prostate cancer Proteins
topic	Marcadors bioquímics Càncer de pròstata Proteïnes Biochemical markers Prostate cancer Proteins
description	Objectives: To apply a systems pathology-based approach to the quantification of nuclear Active β-catenin and human leukocyte antigen class I, and assess the biomarker involvement in a cohort of prostate tumor patients. Results: The systems pathology approach applied allows a precise quantification of the marker expression in the different cell compartments as well as the determination of the areas that coexpress two markers. Our data shows that the accumulation of β-catenin in the nuclear compartment is significantly decreased in the adjacent normal areas when compared to tumor of the same patients (p < 0.001). In conclusion, the application of this novel multiple immunofluorescence assay demonstrates that the upregulation of Active β-catenin is a relatively common feature of prostate tumor development, and further supports the activation of the Wnt/β-catenin pathway in prostate cancer progression.
publishDate	2019
dc.date.none.fl_str_mv	2019 2020 2020 2020
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/164367
url	https://hdl.handle.net/2445/164367
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: https://doi.org/10.1186/s13104-019-4100-z BMC Research Notes, 2019, vol. 12, p. 68 https://doi.org/10.1186/s13104-019-4100-z
dc.rights.none.fl_str_mv	cc-by (c) Puig, Pere et al., 2019 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc-by (c) Puig, Pere et al., 2019 http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	5 p. application/pdf
dc.publisher.none.fl_str_mv	BioMed Central
publisher.none.fl_str_mv	BioMed Central
dc.source.none.fl_str_mv	Articles publicats en revistes (Patologia i Terapèutica Experimental) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.81155

Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer

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