Isolationand characterization of 20 muicrosatellite loci for laurel species (laurus, lauraceae)

• Premise of the study: Microsatellite primers were developed for the evergreen tree Laurus to investigate population genetic structure and patterns of gene flow via animal-dispersed pollen and seeds. • Methods and Results: Twenty polymorphic nuclear microsatellite markers were developed using CA, G...

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Detalles Bibliográficos
Autores: Arroyo, Juan M., Rigueiro, Cristina, Rodríguez, Rocio, Hampe, Arndt, Valido, Alfredo, Rodríguez-Sánchez, Francisco, Jordano, Pedro
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/36442
Acceso en línea:http://hdl.handle.net/10261/36442
Access Level:acceso abierto
Palabra clave:Genetic diversity
Laurus azorica
Laurus nobilis
Polyploidy
SSR.
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spelling Isolationand characterization of 20 muicrosatellite loci for laurel species (laurus, lauraceae)Arroyo, Juan M.Rigueiro, CristinaRodríguez, RocioHampe, ArndtValido, AlfredoRodríguez-Sánchez, FranciscoJordano, PedroJordano, PedroGenetic diversityLaurus azoricaLaurus nobilisPolyploidySSR.• Premise of the study: Microsatellite primers were developed for the evergreen tree Laurus to investigate population genetic structure and patterns of gene flow via animal-dispersed pollen and seeds. • Methods and Results: Twenty polymorphic nuclear microsatellite markers were developed using CA, GA, AAC, and ATG n- enriched genomic libraries. Given the tetraploidy of the sampled populations, we analyzed our data both as dominant loci and as codominant genotypic data to calculate allele frequencies and genetic diversity. A total of 196 and 222 alleles were found in 37 Mediterranean (L. nobilis) and 26 Macaronesian islands (L. azorica) individuals, respectively. • Conclusions: Levels of polymorphism of the reported markers are adequate for studies of diversity and parentage in natural populations of this Tertiary relict tree.Peer reviewedBotanical Society of America201120112010info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/36442reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.amjbot.org/content/97/5/e26.full.pdf+htmlinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/364422026-05-22T06:33:51Z
dc.title.none.fl_str_mv Isolationand characterization of 20 muicrosatellite loci for laurel species (laurus, lauraceae)
title Isolationand characterization of 20 muicrosatellite loci for laurel species (laurus, lauraceae)
spellingShingle Isolationand characterization of 20 muicrosatellite loci for laurel species (laurus, lauraceae)
Arroyo, Juan M.
Genetic diversity
Laurus azorica
Laurus nobilis
Polyploidy
SSR.
title_short Isolationand characterization of 20 muicrosatellite loci for laurel species (laurus, lauraceae)
title_full Isolationand characterization of 20 muicrosatellite loci for laurel species (laurus, lauraceae)
title_fullStr Isolationand characterization of 20 muicrosatellite loci for laurel species (laurus, lauraceae)
title_full_unstemmed Isolationand characterization of 20 muicrosatellite loci for laurel species (laurus, lauraceae)
title_sort Isolationand characterization of 20 muicrosatellite loci for laurel species (laurus, lauraceae)
dc.creator.none.fl_str_mv Arroyo, Juan M.
Rigueiro, Cristina
Rodríguez, Rocio
Hampe, Arndt
Valido, Alfredo
Rodríguez-Sánchez, Francisco
Jordano, Pedro
Jordano, Pedro
author Arroyo, Juan M.
author_facet Arroyo, Juan M.
Rigueiro, Cristina
Rodríguez, Rocio
Hampe, Arndt
Valido, Alfredo
Rodríguez-Sánchez, Francisco
Jordano, Pedro
author_role author
author2 Rigueiro, Cristina
Rodríguez, Rocio
Hampe, Arndt
Valido, Alfredo
Rodríguez-Sánchez, Francisco
Jordano, Pedro
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Genetic diversity
Laurus azorica
Laurus nobilis
Polyploidy
SSR.
topic Genetic diversity
Laurus azorica
Laurus nobilis
Polyploidy
SSR.
description • Premise of the study: Microsatellite primers were developed for the evergreen tree Laurus to investigate population genetic structure and patterns of gene flow via animal-dispersed pollen and seeds. • Methods and Results: Twenty polymorphic nuclear microsatellite markers were developed using CA, GA, AAC, and ATG n- enriched genomic libraries. Given the tetraploidy of the sampled populations, we analyzed our data both as dominant loci and as codominant genotypic data to calculate allele frequencies and genetic diversity. A total of 196 and 222 alleles were found in 37 Mediterranean (L. nobilis) and 26 Macaronesian islands (L. azorica) individuals, respectively. • Conclusions: Levels of polymorphism of the reported markers are adequate for studies of diversity and parentage in natural populations of this Tertiary relict tree.
publishDate 2010
dc.date.none.fl_str_mv 2010
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/36442
url http://hdl.handle.net/10261/36442
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.amjbot.org/content/97/5/e26.full.pdf+html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Botanical Society of America
publisher.none.fl_str_mv Botanical Society of America
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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