Isolationand characterization of 20 muicrosatellite loci for laurel species (laurus, lauraceae)
• Premise of the study: Microsatellite primers were developed for the evergreen tree Laurus to investigate population genetic structure and patterns of gene flow via animal-dispersed pollen and seeds. • Methods and Results: Twenty polymorphic nuclear microsatellite markers were developed using CA, G...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/36442 |
| Acceso en línea: | http://hdl.handle.net/10261/36442 |
| Access Level: | acceso abierto |
| Palabra clave: | Genetic diversity Laurus azorica Laurus nobilis Polyploidy SSR. |
| Sumario: | • Premise of the study: Microsatellite primers were developed for the evergreen tree Laurus to investigate population genetic structure and patterns of gene flow via animal-dispersed pollen and seeds. • Methods and Results: Twenty polymorphic nuclear microsatellite markers were developed using CA, GA, AAC, and ATG n- enriched genomic libraries. Given the tetraploidy of the sampled populations, we analyzed our data both as dominant loci and as codominant genotypic data to calculate allele frequencies and genetic diversity. A total of 196 and 222 alleles were found in 37 Mediterranean (L. nobilis) and 26 Macaronesian islands (L. azorica) individuals, respectively. • Conclusions: Levels of polymorphism of the reported markers are adequate for studies of diversity and parentage in natural populations of this Tertiary relict tree. |
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