Combining stable isotopes and biochemical markers to assess organic contamination in transplanted mussels Mytilus galloprovincialis
Marine pollution and water quality are evaluated on direct measurements of the abiotic variables and also on bioaccumulation measurements of chemical contaminants in marine organisms. Measuring the same biomarkers in different localities simultaneously gives information about the pollution states an...
| Autores: | , , , , |
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| Tipo de recurso: | otro |
| Fecha de publicación: | 2011 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:dnet:digitalcsic_::40590e43dbb83afb5f31a7f3d334bf7a |
| Acceso en línea: | http://hdl.handle.net/10261/317437 |
| Access Level: | acceso abierto |
| Palabra clave: | Medio Marino Caging marine mussels Centro Oceanográfico de Baleares PAHs PCBs Stable isotopes Biomarkers Organic pollution |
| Sumario: | Marine pollution and water quality are evaluated on direct measurements of the abiotic variables and also on bioaccumulation measurements of chemical contaminants in marine organisms. Measuring the same biomarkers in different localities simultaneously gives information about the pollution states and provides a better comprehension of the mechanistic model of action of environmental pollutants on the organisms. The use of biomarkers to evaluate stressful situations is widely extended in bivalves. In the current work, organic compound concentrations (dichlorodiphenyltrichloroethane isomers, dioxins, PCBs and PAHs), antioxidant biomarkers (malondialdehyde, catalase, glutathione peroxidase, superoxide dismutase and glutathione reductase) and isotopic composition (15N and 13C) were measured in the digestive gland and gill tissues of the mussel Mytilus galloprovincialis in coastal waters of the Balearic Islands (Western Mediterranean) in order to assess pollution levels in these waters. The highest concentrations of PAHs corresponded to naphthalene, acenaphthylene, fluorene and phenanthrene, with the harbours of Santa Eulàlia and Eïvissa having the highest levels of PAHs. Oxidative stress and biomarkers are used as indicators of pollution exposure, showing that pollution can not evidence exposure effects, while the antioxidant responses can change with time. In the current work, the existence of pollution was indicated by the positive correlation between the concentrations of the lighter PCBs in the digestive gland of the mussels and catalase and glutathione reductase enzyme activities. Gills showed a correlation between the lighter PCBs and superoxide dismutase activity, indicating the bioaccumulation of these organic compounds. Carbon and nitrogen isotopic signatures showed a clear trend for differences in tissue distribution among the studied localities, with the digestive gland being more enriched in carbon and nitrogen than the gills. PCA for biomarkers also showed that tissues responded differently at sampling stations. The presence of pollutants could be the responsible for the changes described in the isotopic composition and in the antioxidant defences of the mussel M. galloprovincialis in waters of the Balearic Islands. The correlations between organic pollutants and the isotopic composition and biomarkers in M. galloprovincialis suggest that these measures could represent a good proxy for evaluation of contamination, additional to the chemical characterisation. |
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