MicroRNAs as predictors and therapy for prostate cancer

Prostate cancer (PC) is the most common neoplasia among males from developed countries and the second leading cause of death from cancer in these males. During the last years, serum prostate specific antigen (PSA) has been used as biomarker for PC diagnosis. Although PSA test has increased PC detect...

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Detalhes bibliográficos
Autor: Montes Pérez, Melania
Tipo de documento: tese
Estado:Versão publicada
Data de publicação:2015
País:España
Recursos:CBUC, CESCA
Repositório:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/323366
Acesso em linha:http://hdl.handle.net/10803/323366
Access Level:Acceso aberto
Palavra-chave:MicroRNA
Urine
Prostate cancer
Ciències de la Salut
576
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spelling MicroRNAs as predictors and therapy for prostate cancerMontes Pérez, MelaniaMicroRNAUrineProstate cancerCiències de la Salut576Prostate cancer (PC) is the most common neoplasia among males from developed countries and the second leading cause of death from cancer in these males. During the last years, serum prostate specific antigen (PSA) has been used as biomarker for PC diagnosis. Although PSA test has increased PC detection, it has a low specificity and leads to over-diagnosis and over-treatment; therefore, it is necessary to improve the current diagnostic methods for PC. In the past decade, urine has received more and more attention for its simplicity, as well as its potential use in identifying new biomarkers as non-invasive detection method. Deregulation of microRNAs (miRNAs) in serum/plasma or tissue has been associated with many diseases including PC, suggesting the possible use of miRNAs as diagnostic biomarkers also in other body fluids. The intent of my PhD research program was to evaluate if miRNAs known to be deregulated in blood or tissue for PC could also be detected in urine from cellular fraction, and characterize the function of the most promising of them. By analysing a large cohort of post-digital rectal exam (DRE) urine samples, we have validated a miRNA panel consisting of 3 urinary miRNAs (miR-648, miR-147b and miR-193a-5p) with a predictive value of area under the curve (AUC): 0,74 In addition, we have determined that miRNAs-miR-28 and miR-210 are under-expressed in PC urine and associated with PC development and progression, mainly affecting PC cell proliferation and migration. Overall, this work introduces novel candidate miRNAs that are aberrantly expressed in PC as diagnostic biomarkers, and perhaps aids in defining how miRNAs might one day serve as anti-cancer therapeutic agents.Universitat Autònoma de BarcelonaReventós i Puigjaner, JaumeMorote Robles, JuanOlivan Riera, MireiaUniversitat Autònoma de Barcelona. Departament de Medicina201520152015info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersion179 p.application/pdfapplication/pdfhttp://hdl.handle.net/10803/323366TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésL'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-nd/3.0/es/http://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/3233662026-06-14T12:46:07Z
dc.title.none.fl_str_mv MicroRNAs as predictors and therapy for prostate cancer
title MicroRNAs as predictors and therapy for prostate cancer
spellingShingle MicroRNAs as predictors and therapy for prostate cancer
Montes Pérez, Melania
MicroRNA
Urine
Prostate cancer
Ciències de la Salut
576
title_short MicroRNAs as predictors and therapy for prostate cancer
title_full MicroRNAs as predictors and therapy for prostate cancer
title_fullStr MicroRNAs as predictors and therapy for prostate cancer
title_full_unstemmed MicroRNAs as predictors and therapy for prostate cancer
title_sort MicroRNAs as predictors and therapy for prostate cancer
dc.creator.none.fl_str_mv Montes Pérez, Melania
author Montes Pérez, Melania
author_facet Montes Pérez, Melania
author_role author
dc.contributor.none.fl_str_mv Reventós i Puigjaner, Jaume
Morote Robles, Juan
Olivan Riera, Mireia
Universitat Autònoma de Barcelona. Departament de Medicina
dc.subject.none.fl_str_mv MicroRNA
Urine
Prostate cancer
Ciències de la Salut
576
topic MicroRNA
Urine
Prostate cancer
Ciències de la Salut
576
description Prostate cancer (PC) is the most common neoplasia among males from developed countries and the second leading cause of death from cancer in these males. During the last years, serum prostate specific antigen (PSA) has been used as biomarker for PC diagnosis. Although PSA test has increased PC detection, it has a low specificity and leads to over-diagnosis and over-treatment; therefore, it is necessary to improve the current diagnostic methods for PC. In the past decade, urine has received more and more attention for its simplicity, as well as its potential use in identifying new biomarkers as non-invasive detection method. Deregulation of microRNAs (miRNAs) in serum/plasma or tissue has been associated with many diseases including PC, suggesting the possible use of miRNAs as diagnostic biomarkers also in other body fluids. The intent of my PhD research program was to evaluate if miRNAs known to be deregulated in blood or tissue for PC could also be detected in urine from cellular fraction, and characterize the function of the most promising of them. By analysing a large cohort of post-digital rectal exam (DRE) urine samples, we have validated a miRNA panel consisting of 3 urinary miRNAs (miR-648, miR-147b and miR-193a-5p) with a predictive value of area under the curve (AUC): 0,74 In addition, we have determined that miRNAs-miR-28 and miR-210 are under-expressed in PC urine and associated with PC development and progression, mainly affecting PC cell proliferation and migration. Overall, this work introduces novel candidate miRNAs that are aberrantly expressed in PC as diagnostic biomarkers, and perhaps aids in defining how miRNAs might one day serve as anti-cancer therapeutic agents.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10803/323366
url http://hdl.handle.net/10803/323366
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 179 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universitat Autònoma de Barcelona
publisher.none.fl_str_mv Universitat Autònoma de Barcelona
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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