Olive oil quality and authencity methods based on sterol analysis: a review

Virgin olive oil (VOO) is highly regarded worldwide not only for its distinctive sensory characteristics but also for its widely recognized health benefits, positioning it as one of the most valuable vegetable oils. Nevertheless, these desirable properties also make VOO a frequent target of food fra...

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Bibliographic Details
Authors: Caño-Carrillo, Irene, Gilbert-López, Bienvenida, Ruiz-Samblás, Cristina, García-Reyes, Juan F.
Format: article
Status:Versión aceptada para publicación
Publication Date:2026
Country:España
Institution:Universidad de Murcia
Repository:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
OAI Identifier:oai:dnet:ruja________::1f3677c2b082d15bfc996396872b615b
Online Access:https://doi.org/10.1016/j.trac.2026.118852
https://hdl.handle.net/10953/7763
Access Level:Open access
Keyword:Virgin olive oil, sterols, authentication, food quality, fraud
2301.03
2301.10
Description
Summary:Virgin olive oil (VOO) is highly regarded worldwide not only for its distinctive sensory characteristics but also for its widely recognized health benefits, positioning it as one of the most valuable vegetable oils. Nevertheless, these desirable properties also make VOO a frequent target of food fraud, including issues related to the verification of geographical origin, mislabeling of the commercial category, or blending with other vegetable oils. Sterols, a group of minor compounds within the unsaponifiable fraction of olive oil, are considered key markers for evaluating both quality and authenticity. Several factors could influence the sterol composition, such as the geographical region, olive cultivar, and processing techniques. Gas chromatography (GC) coupled with flame ionization detection (FID) or mass spectrometry (MS) yet remains the most commonly used standard approach for sterols analysis, despite certain inherent limitations. However, liquid chromatography (LC), particularly when combined with MS, has also gained increasing interest as an effective alternative. Additionally, methods including ambient MS or spectroscopy have also been proposed for sterol profiling. This review aims to provide an overview of the role of sterols in ensuring VOO quality and authenticity, current methodologies used for their analysis, as well as key markers for detecting fraudulent practices in olive oil.