Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis

[EN]Technical problems intrinsic to the purification of preribosome intermediates have limited our understanding of ribosome biosynthesis in humans. Addressing this issue is important given the implication of this biological process in human disease. Here we report a preribosome purification and tag...

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Detalhes bibliográficos
Autores: Nieto, Blanca, Gaspar, Sonia G, Moriggi, Giulia, Pestov, Dimitri G, Bustelo, Xosé R., Dosil Castro, Mercedes
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/168969
Acesso em linha:http://hdl.handle.net/10366/168969
Access Level:acceso abierto
Palavra-chave:Ribosome maturation
human ribosome
preribosome
40S ribosomal subunit
pre-rRNA
human nucleolus
RNA
Ribosomal Proteins
Staining and Labeling
Exoribonucleases
Humans
Cell Nucleolus
Cell Line
HCT116 Cells
HeLa Cells
RNA Precursors
Ribosome Subunits
Nuclear Proteins
2407 Biología Celular
2415 Biología Molecular
2409 Genética
2302.05 Biosíntesis
precursores del ARN
nucleolo celular
humanos
tinción y etiquetado
línea celular
ARN
células HCT116
células HeLa
subunidades ribosómicas
proteínas ribosómicas
exorribonucleasas
proteínas nucleares
id ES_5c3c3c8bf1c857389aed0b45a5daba05
oai_identifier_str oai:gredos.usal.es:10366/168969
network_acronym_str ES
network_name_str España
repository_id_str
spelling Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesisNieto, BlancaGaspar, Sonia GMoriggi, GiuliaPestov, Dimitri GBustelo, Xosé R.Dosil Castro, MercedesRibosome maturationhuman ribosomepreribosome40S ribosomal subunitpre-rRNAhuman nucleolusRNARibosomal ProteinsStaining and LabelingExoribonucleasesHumansCell NucleolusCell LineHCT116 CellsHeLa CellsRNA PrecursorsRibosome SubunitsNuclear Proteins2407 Biología Celular2415 Biología Molecular2409 Genética2302.05 Biosíntesisprecursores del ARNnucleolo celularhumanostinción y etiquetadolínea celularARNcélulas HCT116células HeLasubunidades ribosómicasproteínas ribosómicasexorribonucleasasproteínas nucleares[EN]Technical problems intrinsic to the purification of preribosome intermediates have limited our understanding of ribosome biosynthesis in humans. Addressing this issue is important given the implication of this biological process in human disease. Here we report a preribosome purification and tagging strategy that overcomes some of the existing technical difficulties. Using these tools, we find that the pre-40S precursors go through two distinct maturation phases inside the nucleolus and follow a regulatory step that precedes late maturation in the cytoplasm. This regulatory step entails the intertwined actions of both PARN (a metazoan-specific ribonuclease) and RRP12 (a phylogenetically conserved 40S biogenesis factor that has acquired additional functional features in higher eukaryotes). Together, these results demonstrate the usefulness of this purification method for the dissection of ribosome biogenesis in human cells. They also identify distinct maturation stages and metazoan-specific regulatory mechanisms involved in the generation of the human 40S ribosomal subunit.Springer202620262020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/168969reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésBFU2014-52729-PBFU2017- 88192-PFS/36-2017SAF2015-64556-R)GC16173472GARCAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1689692026-06-07T06:28:51Z
dc.title.none.fl_str_mv Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
title Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
spellingShingle Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
Nieto, Blanca
Ribosome maturation
human ribosome
preribosome
40S ribosomal subunit
pre-rRNA
human nucleolus
RNA
Ribosomal Proteins
Staining and Labeling
Exoribonucleases
Humans
Cell Nucleolus
Cell Line
HCT116 Cells
HeLa Cells
RNA Precursors
Ribosome Subunits
Nuclear Proteins
2407 Biología Celular
2415 Biología Molecular
2409 Genética
2302.05 Biosíntesis
precursores del ARN
nucleolo celular
humanos
tinción y etiquetado
línea celular
ARN
células HCT116
células HeLa
subunidades ribosómicas
proteínas ribosómicas
exorribonucleasas
proteínas nucleares
title_short Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
title_full Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
title_fullStr Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
title_full_unstemmed Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
title_sort Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
dc.creator.none.fl_str_mv Nieto, Blanca
Gaspar, Sonia G
Moriggi, Giulia
Pestov, Dimitri G
Bustelo, Xosé R.
Dosil Castro, Mercedes
author Nieto, Blanca
author_facet Nieto, Blanca
Gaspar, Sonia G
Moriggi, Giulia
Pestov, Dimitri G
Bustelo, Xosé R.
Dosil Castro, Mercedes
author_role author
author2 Gaspar, Sonia G
Moriggi, Giulia
Pestov, Dimitri G
Bustelo, Xosé R.
Dosil Castro, Mercedes
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ribosome maturation
human ribosome
preribosome
40S ribosomal subunit
pre-rRNA
human nucleolus
RNA
Ribosomal Proteins
Staining and Labeling
Exoribonucleases
Humans
Cell Nucleolus
Cell Line
HCT116 Cells
HeLa Cells
RNA Precursors
Ribosome Subunits
Nuclear Proteins
2407 Biología Celular
2415 Biología Molecular
2409 Genética
2302.05 Biosíntesis
precursores del ARN
nucleolo celular
humanos
tinción y etiquetado
línea celular
ARN
células HCT116
células HeLa
subunidades ribosómicas
proteínas ribosómicas
exorribonucleasas
proteínas nucleares
topic Ribosome maturation
human ribosome
preribosome
40S ribosomal subunit
pre-rRNA
human nucleolus
RNA
Ribosomal Proteins
Staining and Labeling
Exoribonucleases
Humans
Cell Nucleolus
Cell Line
HCT116 Cells
HeLa Cells
RNA Precursors
Ribosome Subunits
Nuclear Proteins
2407 Biología Celular
2415 Biología Molecular
2409 Genética
2302.05 Biosíntesis
precursores del ARN
nucleolo celular
humanos
tinción y etiquetado
línea celular
ARN
células HCT116
células HeLa
subunidades ribosómicas
proteínas ribosómicas
exorribonucleasas
proteínas nucleares
description [EN]Technical problems intrinsic to the purification of preribosome intermediates have limited our understanding of ribosome biosynthesis in humans. Addressing this issue is important given the implication of this biological process in human disease. Here we report a preribosome purification and tagging strategy that overcomes some of the existing technical difficulties. Using these tools, we find that the pre-40S precursors go through two distinct maturation phases inside the nucleolus and follow a regulatory step that precedes late maturation in the cytoplasm. This regulatory step entails the intertwined actions of both PARN (a metazoan-specific ribonuclease) and RRP12 (a phylogenetically conserved 40S biogenesis factor that has acquired additional functional features in higher eukaryotes). Together, these results demonstrate the usefulness of this purification method for the dissection of ribosome biogenesis in human cells. They also identify distinct maturation stages and metazoan-specific regulatory mechanisms involved in the generation of the human 40S ribosomal subunit.
publishDate 2020
dc.date.none.fl_str_mv 2020
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/168969
url http://hdl.handle.net/10366/168969
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv BFU2014-52729-P
BFU2017- 88192-P
FS/36-2017
SAF2015-64556-R)
GC16173472GARC
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408886801301504
score 15.812429

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