Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen

T cells form immunological synapses with professional antigen-presenting cells (APCs) resulting in T cell activation and the acquisition of peptide antigen-MHC (pMHC) complexes from the plasma membrane of the APC. They thus become APCs themselves. We investigate the functional outcome of T-T cell an...

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Autores: Boccasavia, Viola L., Bovolenta, Elena R., Villanueva, Alberto, Borroto Revuelta, Aldo, Oeste, Clara L., Santen, Hisse M. van, Prieto López, Cristina, Alonso-López, D., Díaz-Muñoz, Manuel D., Batista, Facundo D., Alarcón, Balbino
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/241754
Acesso em linha:http://hdl.handle.net/10261/241754
Access Level:acceso abierto
Palavra-chave:Antigen presentation by T cells
Trogocytosis
Th17
Treg
Limiting antigen
Vaccine dosing
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spelling Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigenBoccasavia, Viola L.Bovolenta, Elena R.Villanueva, AlbertoBorroto Revuelta, AldoOeste, Clara L.Santen, Hisse M. vanPrieto López, CristinaAlonso-López, D.Díaz-Muñoz, Manuel D.Batista, Facundo D.Alarcón, BalbinoAntigen presentation by T cellsTrogocytosisTh17TregLimiting antigenVaccine dosingT cells form immunological synapses with professional antigen-presenting cells (APCs) resulting in T cell activation and the acquisition of peptide antigen-MHC (pMHC) complexes from the plasma membrane of the APC. They thus become APCs themselves. We investigate the functional outcome of T-T cell antigen presentation by CD4 T cells and find that the antigen-presenting T cells (Tpres) predominantly differentiate into regulatory T cells (Treg), whereas T cells that have been stimulated by Tpres cells predominantly differentiate into Th17 pro-inflammatory cells. Using mice deficient in pMHC uptake by T cells, we show that T-T antigen presentation is important for the development of experimental autoimmune encephalitis and Th17 cell differentiation in vivo. By varying the professional APC:T cell ratio, we can modulate Treg versus Th17 differentiation in vitro and in vivo, suggesting that T-T antigen presentation underlies proinflammatory responses in conditions of antigen scarcity.This work was funded by grant no. SAF2016-76394-R, from the CICYT, by grant no. S2017/BMD-3671 from the Comunidad de Madrid, and by the European Research Council ERC 2013-Advanced Grant 334763 “NOVARIPP” (to B.A.). V.L.B. was supported by an ITN-Marie Curie Fellowship. CBMSO acknowledges the support of the Fundación Ramón Areces.Cell PressMinisterio de Economía y Competitividad (España)Comunidad de MadridEuropean Research CouncilEuropean CommissionFundación Ramón ArecesConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/241754reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-76394-RS2017/BMD-3671/INFLAMUNE-CMinfo:eu-repo/grantAgreement/EC/FP7/334763http://dx.doi.org/10.1016/j.celrep.2021.108861Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2417542026-05-22T06:33:51Z
dc.title.none.fl_str_mv Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen
title Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen
spellingShingle Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen
Boccasavia, Viola L.
Antigen presentation by T cells
Trogocytosis
Th17
Treg
Limiting antigen
Vaccine dosing
title_short Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen
title_full Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen
title_fullStr Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen
title_full_unstemmed Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen
title_sort Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen
dc.creator.none.fl_str_mv Boccasavia, Viola L.
Bovolenta, Elena R.
Villanueva, Alberto
Borroto Revuelta, Aldo
Oeste, Clara L.
Santen, Hisse M. van
Prieto López, Cristina
Alonso-López, D.
Díaz-Muñoz, Manuel D.
Batista, Facundo D.
Alarcón, Balbino
author Boccasavia, Viola L.
author_facet Boccasavia, Viola L.
Bovolenta, Elena R.
Villanueva, Alberto
Borroto Revuelta, Aldo
Oeste, Clara L.
Santen, Hisse M. van
Prieto López, Cristina
Alonso-López, D.
Díaz-Muñoz, Manuel D.
Batista, Facundo D.
Alarcón, Balbino
author_role author
author2 Bovolenta, Elena R.
Villanueva, Alberto
Borroto Revuelta, Aldo
Oeste, Clara L.
Santen, Hisse M. van
Prieto López, Cristina
Alonso-López, D.
Díaz-Muñoz, Manuel D.
Batista, Facundo D.
Alarcón, Balbino
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
European Research Council
European Commission
Fundación Ramón Areces
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antigen presentation by T cells
Trogocytosis
Th17
Treg
Limiting antigen
Vaccine dosing
topic Antigen presentation by T cells
Trogocytosis
Th17
Treg
Limiting antigen
Vaccine dosing
description T cells form immunological synapses with professional antigen-presenting cells (APCs) resulting in T cell activation and the acquisition of peptide antigen-MHC (pMHC) complexes from the plasma membrane of the APC. They thus become APCs themselves. We investigate the functional outcome of T-T cell antigen presentation by CD4 T cells and find that the antigen-presenting T cells (Tpres) predominantly differentiate into regulatory T cells (Treg), whereas T cells that have been stimulated by Tpres cells predominantly differentiate into Th17 pro-inflammatory cells. Using mice deficient in pMHC uptake by T cells, we show that T-T antigen presentation is important for the development of experimental autoimmune encephalitis and Th17 cell differentiation in vivo. By varying the professional APC:T cell ratio, we can modulate Treg versus Th17 differentiation in vitro and in vivo, suggesting that T-T antigen presentation underlies proinflammatory responses in conditions of antigen scarcity.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/241754
url http://hdl.handle.net/10261/241754
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-76394-R
S2017/BMD-3671/INFLAMUNE-CM
info:eu-repo/grantAgreement/EC/FP7/334763
http://dx.doi.org/10.1016/j.celrep.2021.108861
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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