Is there any relationship between gene expression of tumor antigens and CD4+ T cells in multiple myeloma?

Aim: The present study aimed at correlating the expression of cancer/testis antigens (CTAs) with the expression of genes related to tumor‐infiltrating T cells. Materials & methods:MAGE‐C1/CT‐7, MAGEA3/6, NY‐ESO‐1, LAGE‐1 and GAGE expression were evaluated in 46 bone marrow multiple myeloma (MM)...

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Detalles Bibliográficos
Autores: Braga, Walter MT, Silva, Bruna R da, Alves, Veruska LF, Atanackovic, Djordje, Colleoni, Gisele WB, ADRIANA BRUSCATO BORTOLUZZO
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Brasil
Institución:Instituição de Ensino Superior e de Pesquisa (INSPER)
Repositorio:Repositório Institucional da INSPER
Idioma:inglés
OAI Identifier:oai:repositorio.insper.edu.br:11224/3986
Acceso en línea:https://repositorio.insper.edu.br/handle/11224/3986
https://doi.org/10.2217/imt.14.24
Access Level:acceso abierto
Palabra clave:cancer/testis antigen
multiple myeloma
Th17
Treg
Descripción
Sumario:Aim: The present study aimed at correlating the expression of cancer/testis antigens (CTAs) with the expression of genes related to tumor‐infiltrating T cells. Materials & methods:MAGE‐C1/CT‐7, MAGEA3/6, NY‐ESO‐1, LAGE‐1 and GAGE expression were evaluated in 46 bone marrow multiple myeloma (MM) aspirates by RT‐PCR. Expression of FOXP3/CTLA4 and RORyt, as markers for Tregs and Th17 cells, respectively, was investigated by quantitative PCR. Results:MAGEC1/CT7 was expressed in 66% of MM samples. We did not find correlation between the presence of single CTA and expression of CTLA4 or RORyt neither expression of CD4+ T‐cell markers and the number of CTA simultaneously expressed in the tumor. However, we did observe a correlation between the percentage of plasma cells and the number of CTAs expressed in the patients' bone marrow. Conclusion: Although CTAs and immunomodulatory CD4+ T cells represent potential targets for immunotherapy in MM, we did not find association among expression of such genes in MM