Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity
[EN] Cell transplantation therapies in the nervous system are frequently hampered by glial scarring and cell drain from the damaged site, among others. To improve this situation, new biomaterials may be of help. Here, novel single-channel tubular conduits based on hyaluronic acid (HA) with and witho...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/89409 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/89409 |
| Access Level: | acceso abierto |
| Palabra clave: | Nervous system regeneration Hyaluronic acid Schwann cell Protective barrier Cell sheet Electron Microscopy Service of the UPV TECNOLOGIA ELECTRONICA MAQUINAS Y MOTORES TERMICOS |
| id |
ES_59ffb00a211104e33a8e7c042a570bd2 |
|---|---|
| oai_identifier_str |
oai:riunet.upv.es:10251/89409 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosityVilariño-Feltrer, Guillermo|||0000-0002-3063-3808Martínez-Ramos, Cristina|||0000-0002-6540-4714Vallés Lluch, Ana|||0000-0002-7896-8666Moratal, David|||0000-0002-2825-3646Monleón Pradas, Manuel|||0000-0001-6457-0414Monleon De la Fuente, AdrianBarcia Albacar, Juan AntonioNervous system regenerationHyaluronic acidSchwann cellProtective barrierCell sheetElectron Microscopy Service of the UPVTECNOLOGIA ELECTRONICAMAQUINAS Y MOTORES TERMICOS[EN] Cell transplantation therapies in the nervous system are frequently hampered by glial scarring and cell drain from the damaged site, among others. To improve this situation, new biomaterials may be of help. Here, novel single-channel tubular conduits based on hyaluronic acid (HA) with and without poly-l-lactide acid fibers in their lumen were fabricated. Rat Schwann cells were seeded within the conduits and cultured for 10days. The conduits possessed a three-layered porous structure that impeded the leakage of the cells seeded in their interior and made them impervious to cell invasion from the exterior, while allowing free transport of nutrients and other molecules needed for cell survival. The channel's surface acted as a template for the formation of a cylindrical sheath-like tapestry of Schwann cells continuously spanning the whole length of the lumen. Schwann-cell tubes having a diameter of around 0.5mm and variable lengths can thus be generated. This structure is not found in nature and represents a truly engineered tissue, the outcome of the specific cell-material interactions. The conduits might be useful to sustain and protect cells for transplantation, and the biohybrids here described, together with neuronal precursors, might be of help in building bridges across significant distances in the central and peripheral nervous system.The authors acknowledge financing through projects MAT2011-28791-C03-02 and 03, and ERA-NET NEURON project PRI-PIMNEU-2011-1372. We thank the Cytomics Core Facility at Principe Felipe Research Center (CIPF, Valencia, Spain) for their support and advice in flow cytometry experiments, and the Electron Microscopy Service at the UPV, where the SEM images were obtained. The authors thankfully acknowledge the reviewers' comments, which have helped to improve the clarity of the paper's presentation.Departamento de Ingeniería ElectrónicaDepartamento de Termodinámica AplicadaCentro de Biomateriales e Ingeniería TisularEscuela Técnica Superior de Ingeniería IndustrialMinisterio de Ciencia e InnovaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20162016-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/89409reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 MAT2011-28791-C03-02 MATERIALES DE SOPORTE Y LIBERACION CONTROLADA PARA LA REGENERACION DE ESTRUCTURAS NEURALES AFECTADAS POR ICTUSMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 MAT2011-28791-C03-03 CONSTRUCTOS PARA LA REGENERACION GUIADA DE ESTRUCTURAS DEL SISTEMA NERVIOSO CENTRALMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 PRI-PIMNEU-2011-1372 MATERIALES BIFUNCIONALES PARA LA REGENERACION NEURAL DE AREAS AFECTADAS POR ICTUSopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/894092026-06-13T07:49:27Z |
| dc.title.none.fl_str_mv |
Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity |
| title |
Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity |
| spellingShingle |
Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity Vilariño-Feltrer, Guillermo|||0000-0002-3063-3808 Nervous system regeneration Hyaluronic acid Schwann cell Protective barrier Cell sheet Electron Microscopy Service of the UPV TECNOLOGIA ELECTRONICA MAQUINAS Y MOTORES TERMICOS |
| title_short |
Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity |
| title_full |
Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity |
| title_fullStr |
Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity |
| title_full_unstemmed |
Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity |
| title_sort |
Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity |
| dc.creator.none.fl_str_mv |
Vilariño-Feltrer, Guillermo|||0000-0002-3063-3808 Martínez-Ramos, Cristina|||0000-0002-6540-4714 Vallés Lluch, Ana|||0000-0002-7896-8666 Moratal, David|||0000-0002-2825-3646 Monleón Pradas, Manuel|||0000-0001-6457-0414 Monleon De la Fuente, Adrian Barcia Albacar, Juan Antonio |
| author |
Vilariño-Feltrer, Guillermo|||0000-0002-3063-3808 |
| author_facet |
Vilariño-Feltrer, Guillermo|||0000-0002-3063-3808 Martínez-Ramos, Cristina|||0000-0002-6540-4714 Vallés Lluch, Ana|||0000-0002-7896-8666 Moratal, David|||0000-0002-2825-3646 Monleón Pradas, Manuel|||0000-0001-6457-0414 Monleon De la Fuente, Adrian Barcia Albacar, Juan Antonio |
| author_role |
author |
| author2 |
Martínez-Ramos, Cristina|||0000-0002-6540-4714 Vallés Lluch, Ana|||0000-0002-7896-8666 Moratal, David|||0000-0002-2825-3646 Monleón Pradas, Manuel|||0000-0001-6457-0414 Monleon De la Fuente, Adrian Barcia Albacar, Juan Antonio |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Ingeniería Electrónica Departamento de Termodinámica Aplicada Centro de Biomateriales e Ingeniería Tisular Escuela Técnica Superior de Ingeniería Industrial Ministerio de Ciencia e Innovación Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Nervous system regeneration Hyaluronic acid Schwann cell Protective barrier Cell sheet Electron Microscopy Service of the UPV TECNOLOGIA ELECTRONICA MAQUINAS Y MOTORES TERMICOS |
| topic |
Nervous system regeneration Hyaluronic acid Schwann cell Protective barrier Cell sheet Electron Microscopy Service of the UPV TECNOLOGIA ELECTRONICA MAQUINAS Y MOTORES TERMICOS |
| description |
[EN] Cell transplantation therapies in the nervous system are frequently hampered by glial scarring and cell drain from the damaged site, among others. To improve this situation, new biomaterials may be of help. Here, novel single-channel tubular conduits based on hyaluronic acid (HA) with and without poly-l-lactide acid fibers in their lumen were fabricated. Rat Schwann cells were seeded within the conduits and cultured for 10days. The conduits possessed a three-layered porous structure that impeded the leakage of the cells seeded in their interior and made them impervious to cell invasion from the exterior, while allowing free transport of nutrients and other molecules needed for cell survival. The channel's surface acted as a template for the formation of a cylindrical sheath-like tapestry of Schwann cells continuously spanning the whole length of the lumen. Schwann-cell tubes having a diameter of around 0.5mm and variable lengths can thus be generated. This structure is not found in nature and represents a truly engineered tissue, the outcome of the specific cell-material interactions. The conduits might be useful to sustain and protect cells for transplantation, and the biohybrids here described, together with neuronal precursors, might be of help in building bridges across significant distances in the central and peripheral nervous system. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2016-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/89409 |
| url |
https://riunet.upv.es/handle/10251/89409 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 MAT2011-28791-C03-02 MATERIALES DE SOPORTE Y LIBERACION CONTROLADA PARA LA REGENERACION DE ESTRUCTURAS NEURALES AFECTADAS POR ICTUS Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 MAT2011-28791-C03-03 CONSTRUCTOS PARA LA REGENERACION GUIADA DE ESTRUCTURAS DEL SISTEMA NERVIOSO CENTRAL Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 PRI-PIMNEU-2011-1372 MATERIALES BIFUNCIONALES PARA LA REGENERACION NEURAL DE AREAS AFECTADAS POR ICTUS |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.source.none.fl_str_mv |
reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
| instname_str |
Universitat Politècnica de València (UPV) |
| reponame_str |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| collection |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869408665190006784 |
| score |
15,300724 |