A facile method to obtain hydrophilic-hydrophobic hyaluronic acid- polylactic acid bilayer films
[EN] Fostering cell attachment is essential for tissue engineering. However, unwanted cellular interactions increase the risk of post-operative adhesions which can hinder tissue regeneration. Thus, cell-adhesion-promoting and cell-adhesion-inhibitory functions need to be regulated simultaneously. We...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/220024 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/220024 |
| Access Level: | acceso abierto |
| Palabra clave: | Polylactic acid Hyaluronic acid Cell adhesion prevention Schwann cells |
| Sumario: | [EN] Fostering cell attachment is essential for tissue engineering. However, unwanted cellular interactions increase the risk of post-operative adhesions which can hinder tissue regeneration. Thus, cell-adhesion-promoting and cell-adhesion-inhibitory functions need to be regulated simultaneously. We develop a stably joined bilayer film composed of a thin polylactic acid film (cell interactive side) obtained by solvent casting coated with hyaluronic acid (cell antifouling side) crosslinked with divinyl sulphone, seeking to reduce post-operative adhesions after surgery. Both layers were bonded together by a biocompatible nitrocellulose-base adhesive forming a long-term stable bilayer due to the reaction between their chemical groups and the formation of a semi-interpenetrating polymeric network on the amorphous regions of the polylactic acid film. A thorough physicochemical characterisation (morphology, composition, swelling ratio and stability) of this novel type of scaffold was carried out to ensure a comprehensive understanding of its structural properties and performance characteristics. Schwann cell culture on the bilayer film proved that the polylactic acid surface was cell-friendly and promoted cell attachment and proliferation whereas the hyaluronic acid surface prevented initial cell adhesion. |
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