Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes

Innovative technologies as ultrasound-assisted extraction (UAE) (550 W, 60% amplitude, 50 °C) or subcritical water extraction (SWE) (200 °C, 11.7 MPa) were more effective than hot water extractions to obtain β-glucan-enriched fractions from shiitake mushrooms. UAE required longer extraction time (60...

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Detalles Bibliográficos
Autores: Morales, Diego, Smiderle, Fhernanda Ribeiro, Villalva, Marisol, Abreu, Hellen, Rico, Claudia, Iacomini, Marcello, Santoyo Díez, Susana, Soler Rivas, Cristina
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/717290
Acceso en línea:http://hdl.handle.net/10486/717290
https://dx.doi.org/10.1016/j.jff.2019.103446
Access Level:acceso abierto
Palabra clave:Hypocholesterolemic activity
immunomodulatory activity
shiitake mushroom
subcritical water extraction
ultrasound-assisted extraction
β-Glucan
Ciencia y Tecnología de Alimentos
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spelling Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodesMorales, DiegoSmiderle, Fhernanda RibeiroVillalva, MarisolAbreu, HellenRico, ClaudiaIacomini, MarcelloSantoyo Díez, SusanaSoler Rivas, CristinaHypocholesterolemic activityimmunomodulatory activityshiitake mushroomsubcritical water extractionultrasound-assisted extractionβ-GlucanCiencia y Tecnología de AlimentosInnovative technologies as ultrasound-assisted extraction (UAE) (550 W, 60% amplitude, 50 °C) or subcritical water extraction (SWE) (200 °C, 11.7 MPa) were more effective than hot water extractions to obtain β-glucan-enriched fractions from shiitake mushrooms. UAE required longer extraction time (60 min) than SWE (15 min). Combination of UAE + SWE or pre-treatment of the raw material with supercritical CO2 (SFE) (40 °C, 35 MPa, 3 h) before both extractions yielded extracts containing larger β-glucan concentrations. Fluorimetric/colorimetric determinations indicated that obtained fractions contained (1 → 3)- and (1 → 3),(1 → 6)-β-glucans. NMR confirmed their presence as well as (1 → 3)-α-glucans and heteropolymers including mannose and galactose. SWE (15 min), SFE + SWE or UAE + SWE extracts showed larger glucose levels and lower mannose and galactose residues than the other extractions suggesting certain extraction specificity towards β-glucans. They also included more chitin-derivatives than UAE. The extracts obtained after combination of technologies partially retained their immunomodulatory properties but they showed high hypocholesterolemic activities according to in vitro studiesThis research was supported by national R+ D program from the Spanish Ministry of Science and Innovation (project AGL2014-56211-R ) and the regional program from the Community of Madrid, Spain ( S2013/ABI-2728 ).ElsevierFacultad de Ciencias20192019-09-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/717290https://dx.doi.org/10.1016/j.jff.2019.103446reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7172902026-06-23T12:46:27Z
dc.title.none.fl_str_mv Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes
title Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes
spellingShingle Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes
Morales, Diego
Hypocholesterolemic activity
immunomodulatory activity
shiitake mushroom
subcritical water extraction
ultrasound-assisted extraction
β-Glucan
Ciencia y Tecnología de Alimentos
title_short Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes
title_full Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes
title_fullStr Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes
title_full_unstemmed Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes
title_sort Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes
dc.creator.none.fl_str_mv Morales, Diego
Smiderle, Fhernanda Ribeiro
Villalva, Marisol
Abreu, Hellen
Rico, Claudia
Iacomini, Marcello
Santoyo Díez, Susana
Soler Rivas, Cristina
author Morales, Diego
author_facet Morales, Diego
Smiderle, Fhernanda Ribeiro
Villalva, Marisol
Abreu, Hellen
Rico, Claudia
Iacomini, Marcello
Santoyo Díez, Susana
Soler Rivas, Cristina
author_role author
author2 Smiderle, Fhernanda Ribeiro
Villalva, Marisol
Abreu, Hellen
Rico, Claudia
Iacomini, Marcello
Santoyo Díez, Susana
Soler Rivas, Cristina
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Facultad de Ciencias
dc.subject.none.fl_str_mv Hypocholesterolemic activity
immunomodulatory activity
shiitake mushroom
subcritical water extraction
ultrasound-assisted extraction
β-Glucan
Ciencia y Tecnología de Alimentos
topic Hypocholesterolemic activity
immunomodulatory activity
shiitake mushroom
subcritical water extraction
ultrasound-assisted extraction
β-Glucan
Ciencia y Tecnología de Alimentos
description Innovative technologies as ultrasound-assisted extraction (UAE) (550 W, 60% amplitude, 50 °C) or subcritical water extraction (SWE) (200 °C, 11.7 MPa) were more effective than hot water extractions to obtain β-glucan-enriched fractions from shiitake mushrooms. UAE required longer extraction time (60 min) than SWE (15 min). Combination of UAE + SWE or pre-treatment of the raw material with supercritical CO2 (SFE) (40 °C, 35 MPa, 3 h) before both extractions yielded extracts containing larger β-glucan concentrations. Fluorimetric/colorimetric determinations indicated that obtained fractions contained (1 → 3)- and (1 → 3),(1 → 6)-β-glucans. NMR confirmed their presence as well as (1 → 3)-α-glucans and heteropolymers including mannose and galactose. SWE (15 min), SFE + SWE or UAE + SWE extracts showed larger glucose levels and lower mannose and galactose residues than the other extractions suggesting certain extraction specificity towards β-glucans. They also included more chitin-derivatives than UAE. The extracts obtained after combination of technologies partially retained their immunomodulatory properties but they showed high hypocholesterolemic activities according to in vitro studies
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-09-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/717290
https://dx.doi.org/10.1016/j.jff.2019.103446
url http://hdl.handle.net/10486/717290
https://dx.doi.org/10.1016/j.jff.2019.103446
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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