Notas preliminares sobre las técnicas de amplificación y variación de la región lTS (Intemal Transcribed Spacer) en el género Encalypta (Encalyptaceae, Bryophyta)
[EN]Results on DNA amplifications are presented for the Encalypta species present in the Iberian Peninsula. DNA was isolated from protonema obtained from cultures in vitro spores. The internal transcribed spacers 1 and 2, including the 5.8S nrDNA have been amplified. Pair primers ITS5/ITS4, ITS1/ITS...
| Authors: | , |
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| Format: | article |
| Publication Date: | 2001 |
| Country: | España |
| Institution: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repository: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:dnet:digitalcsic_::a3933d1b1137d1bab6ad74e143b15a3a |
| Online Access: | http://hdl.handle.net/10261/45545 |
| Access Level: | Open access |
| Keyword: | Encalyptaceae Musgos Península Ibérica Amplificación ADN ITS |
| Summary: | [EN]Results on DNA amplifications are presented for the Encalypta species present in the Iberian Peninsula. DNA was isolated from protonema obtained from cultures in vitro spores. The internal transcribed spacers 1 and 2, including the 5.8S nrDNA have been amplified. Pair primers ITS5/ITS4, ITS1/ITS4, ITS1/ITS2 and ITS3/ITS4 were tested by using two different amplification protocols. ITS5/ITS4 and ITS1/ITS4 yielded amplimer only from fresh samples, not from herbarium collections, from which only primers ITS1/ITS2 and ITS3/ITS4 amplified successfully each of the ITS regions separately. |
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