Uptake and Survival of African Swine Fever Virus in Mealworm (Tenebrio molitor) and Black Soldier Fly (Hermetia illucens) Larvae

Insect production offers a sustainable source of nutrients for livestock. This comes with a risk for transmission of pathogens from the insects into the livestock sector, including viruses causing serious diseases, such as African swine fever virus (ASFV), classical swine fever virus and foot-and-mo...

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Autores: Olesen, Ann Sofie|||0000-0002-6161-3439, Lazov, Christina Marie|||0000-0003-2852-970X, Lecocq, Antoine|||0000-0002-8013-0221, Accensi Alemany, Francesc|||0000-0001-9068-0377, Jensen, Annette Bruun|||0000-0002-2044-2274, Lohse, Louise|||0000-0002-6769-5447, Rasmussen, Thomas Bruun|||0000-0002-4241-1559, Belsham, Graham J.|||0000-0003-1187-4873, Bøtner, Anette|||0000-0002-0558-0222
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:270873
Acceso en línea:https://ddd.uab.cat/record/270873
https://dx.doi.org/urn:doi:10.3390/pathogens12010047
Access Level:acceso abierto
Palabra clave:African swine fever virus
Black soldier fly
Feed safety
Insect rearing
Mealworm
Virus survival
Virus transmission
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spelling Uptake and Survival of African Swine Fever Virus in Mealworm (Tenebrio molitor) and Black Soldier Fly (Hermetia illucens) LarvaeOlesen, Ann Sofie|||0000-0002-6161-3439Lazov, Christina Marie|||0000-0003-2852-970XLecocq, Antoine|||0000-0002-8013-0221Accensi Alemany, Francesc|||0000-0001-9068-0377Jensen, Annette Bruun|||0000-0002-2044-2274Lohse, Louise|||0000-0002-6769-5447Rasmussen, Thomas Bruun|||0000-0002-4241-1559Belsham, Graham J.|||0000-0003-1187-4873Bøtner, Anette|||0000-0002-0558-0222African swine fever virusBlack soldier flyFeed safetyInsect rearingMealwormVirus survivalVirus transmissionInsect production offers a sustainable source of nutrients for livestock. This comes with a risk for transmission of pathogens from the insects into the livestock sector, including viruses causing serious diseases, such as African swine fever virus (ASFV), classical swine fever virus and foot-and-mouth disease virus. ASFV is known to survive for a long time within animal meat and byproducts. Therefore, we conducted experimental exposure studies of insects to ASFV using larvae of two key insect species produced for food and feed, the mealworm; Tenebrio molitor, and the black soldier fly, Hermetia illucens. The larvae were exposed to ASFV POL/2015/Podlaskie, via oral uptake of serum or spleen material from ASFV-infected pigs. Using qPCR, the amounts of viral DNA present immediately after exposure varied from ~10 4.7 to 10 7.2 genome copies per insect. ASFV DNA was detectable in the larvae of H. illucens for up to 3 days post exposure and in T. molitor larvae for up to 9 days post exposure. To assess the presence of infectious virus within the larvae and with this, the risk of virus transmission via oral consumption, pigs were fed cakes containing larvae exposed to ASFV. Pigs that consumed 50 T. molitor or 50 H. illucens virus-exposed larvae did not become infected with ASFV. Thus, it appears, that in our experimental setting, the risk of ASFV transmission via consumption of unprocessed insect larvae, used as feed, is low. 22022-01-0120222022-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/270873https://dx.doi.org/urn:doi:10.3390/pathogens12010047reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2708732026-06-06T12:50:31Z
dc.title.none.fl_str_mv Uptake and Survival of African Swine Fever Virus in Mealworm (Tenebrio molitor) and Black Soldier Fly (Hermetia illucens) Larvae
title Uptake and Survival of African Swine Fever Virus in Mealworm (Tenebrio molitor) and Black Soldier Fly (Hermetia illucens) Larvae
spellingShingle Uptake and Survival of African Swine Fever Virus in Mealworm (Tenebrio molitor) and Black Soldier Fly (Hermetia illucens) Larvae
Olesen, Ann Sofie|||0000-0002-6161-3439
African swine fever virus
Black soldier fly
Feed safety
Insect rearing
Mealworm
Virus survival
Virus transmission
title_short Uptake and Survival of African Swine Fever Virus in Mealworm (Tenebrio molitor) and Black Soldier Fly (Hermetia illucens) Larvae
title_full Uptake and Survival of African Swine Fever Virus in Mealworm (Tenebrio molitor) and Black Soldier Fly (Hermetia illucens) Larvae
title_fullStr Uptake and Survival of African Swine Fever Virus in Mealworm (Tenebrio molitor) and Black Soldier Fly (Hermetia illucens) Larvae
title_full_unstemmed Uptake and Survival of African Swine Fever Virus in Mealworm (Tenebrio molitor) and Black Soldier Fly (Hermetia illucens) Larvae
title_sort Uptake and Survival of African Swine Fever Virus in Mealworm (Tenebrio molitor) and Black Soldier Fly (Hermetia illucens) Larvae
dc.creator.none.fl_str_mv Olesen, Ann Sofie|||0000-0002-6161-3439
Lazov, Christina Marie|||0000-0003-2852-970X
Lecocq, Antoine|||0000-0002-8013-0221
Accensi Alemany, Francesc|||0000-0001-9068-0377
Jensen, Annette Bruun|||0000-0002-2044-2274
Lohse, Louise|||0000-0002-6769-5447
Rasmussen, Thomas Bruun|||0000-0002-4241-1559
Belsham, Graham J.|||0000-0003-1187-4873
Bøtner, Anette|||0000-0002-0558-0222
author Olesen, Ann Sofie|||0000-0002-6161-3439
author_facet Olesen, Ann Sofie|||0000-0002-6161-3439
Lazov, Christina Marie|||0000-0003-2852-970X
Lecocq, Antoine|||0000-0002-8013-0221
Accensi Alemany, Francesc|||0000-0001-9068-0377
Jensen, Annette Bruun|||0000-0002-2044-2274
Lohse, Louise|||0000-0002-6769-5447
Rasmussen, Thomas Bruun|||0000-0002-4241-1559
Belsham, Graham J.|||0000-0003-1187-4873
Bøtner, Anette|||0000-0002-0558-0222
author_role author
author2 Lazov, Christina Marie|||0000-0003-2852-970X
Lecocq, Antoine|||0000-0002-8013-0221
Accensi Alemany, Francesc|||0000-0001-9068-0377
Jensen, Annette Bruun|||0000-0002-2044-2274
Lohse, Louise|||0000-0002-6769-5447
Rasmussen, Thomas Bruun|||0000-0002-4241-1559
Belsham, Graham J.|||0000-0003-1187-4873
Bøtner, Anette|||0000-0002-0558-0222
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv African swine fever virus
Black soldier fly
Feed safety
Insect rearing
Mealworm
Virus survival
Virus transmission
topic African swine fever virus
Black soldier fly
Feed safety
Insect rearing
Mealworm
Virus survival
Virus transmission
description Insect production offers a sustainable source of nutrients for livestock. This comes with a risk for transmission of pathogens from the insects into the livestock sector, including viruses causing serious diseases, such as African swine fever virus (ASFV), classical swine fever virus and foot-and-mouth disease virus. ASFV is known to survive for a long time within animal meat and byproducts. Therefore, we conducted experimental exposure studies of insects to ASFV using larvae of two key insect species produced for food and feed, the mealworm; Tenebrio molitor, and the black soldier fly, Hermetia illucens. The larvae were exposed to ASFV POL/2015/Podlaskie, via oral uptake of serum or spleen material from ASFV-infected pigs. Using qPCR, the amounts of viral DNA present immediately after exposure varied from ~10 4.7 to 10 7.2 genome copies per insect. ASFV DNA was detectable in the larvae of H. illucens for up to 3 days post exposure and in T. molitor larvae for up to 9 days post exposure. To assess the presence of infectious virus within the larvae and with this, the risk of virus transmission via oral consumption, pigs were fed cakes containing larvae exposed to ASFV. Pigs that consumed 50 T. molitor or 50 H. illucens virus-exposed larvae did not become infected with ASFV. Thus, it appears, that in our experimental setting, the risk of ASFV transmission via consumption of unprocessed insect larvae, used as feed, is low.
publishDate 2022
dc.date.none.fl_str_mv 2
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/270873
https://dx.doi.org/urn:doi:10.3390/pathogens12010047
url https://ddd.uab.cat/record/270873
https://dx.doi.org/urn:doi:10.3390/pathogens12010047
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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