X chromosome inactivation during grasshopper spermatogenesis
Regulation of transcriptional activity during meiosis depends on the interrelated processes of recombination and synapsis. In eutherian mammal spermatocytes, transcription levels change during prophase-I, being low at the onset of meiosis but highly increased from pachytene up to the end of diploten...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad Autónoma de Madrid |
| Repositorio: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.uam.es:10486/704712 |
| Acceso en línea: | http://hdl.handle.net/10486/704712 https://dx.doi.org/10.3390/genes12121844 |
| Access Level: | acceso abierto |
| Palabra clave: | Animals Chromosome Pairing Epigenesis, Genetic Female Gene Silencing Grasshoppers Lysine Male Meiosis Meiotic Prophase I RNA Polymerase II Spermatocytes Spermatogenesis X Chromosome X Chromosome Inactivation Y Chromosome Biología y Biomedicina / Biología |
| id |
ES_56f6fa3b5ed2d4361f9d4674cfecf293 |
|---|---|
| oai_identifier_str |
oai:repositorio.uam.es:10486/704712 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
X chromosome inactivation during grasshopper spermatogenesisViera Vicario, AlbertoParra Catalán, María TeresaArévalo, SaraGarcía de la Vega, CarlosSantos, Juan LuisPage Utrilla, JesúsAnimalsChromosome PairingEpigenesis, GeneticFemaleGene SilencingGrasshoppersLysineMaleMeiosisMeiotic Prophase IRNA Polymerase IISpermatocytesSpermatogenesisX ChromosomeX Chromosome InactivationY ChromosomeBiología y Biomedicina / BiologíaRegulation of transcriptional activity during meiosis depends on the interrelated processes of recombination and synapsis. In eutherian mammal spermatocytes, transcription levels change during prophase-I, being low at the onset of meiosis but highly increased from pachytene up to the end of diplotene. However, X and Y chromosomes, which usually present unsynapsed regions throughout prophase-I in male meiosis, undergo a specific pattern of transcriptional inactivation. The interdependence of synapsis and transcription has mainly been studied in mammals, basically in mouse, but our knowledge in other unrelated phylogenetically species is more limited. To gain new insights on this issue, here we analyzed the relationship between synapsis and transcription in spermatocytes of the grasshopper Eyprepocnemis plorans. Autosomal chromosomes of this species achieve complete synapsis; however, the single X sex chromosome remains always unsynapsed and behaves as a univalent. We studied transcription in meiosis by immunolabeling with RNA polymerase II phosphorylated at serine 2 and found that whereas autosomes are active from leptotene up to diakinesis, the X chromosome is inactive throughout meiosis. This inactivation is accompanied by the accumulation of, at least, two repressive epigenetic modifications: H3 methylated at lysine 9 and H2AX phosphorylated at serine 139. Furthermore, we identified that X chromosome inactivation occurs in premeiotic spermatogonia. Overall, our results indicate: (i) transcription regulation in E. plorans spermatogenesis differs from the canonical pattern found in mammals and (ii) X chromosome inactivation is likely preceded by a process of heterochromatinization before the initiation of meiosisMDPIDepartamento de BiologíaFacultad de Ciencias20212021-11-23research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/704712https://dx.doi.org/10.3390/genes12121844reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7047122026-06-23T12:46:27Z |
| dc.title.none.fl_str_mv |
X chromosome inactivation during grasshopper spermatogenesis |
| title |
X chromosome inactivation during grasshopper spermatogenesis |
| spellingShingle |
X chromosome inactivation during grasshopper spermatogenesis Viera Vicario, Alberto Animals Chromosome Pairing Epigenesis, Genetic Female Gene Silencing Grasshoppers Lysine Male Meiosis Meiotic Prophase I RNA Polymerase II Spermatocytes Spermatogenesis X Chromosome X Chromosome Inactivation Y Chromosome Biología y Biomedicina / Biología |
| title_short |
X chromosome inactivation during grasshopper spermatogenesis |
| title_full |
X chromosome inactivation during grasshopper spermatogenesis |
| title_fullStr |
X chromosome inactivation during grasshopper spermatogenesis |
| title_full_unstemmed |
X chromosome inactivation during grasshopper spermatogenesis |
| title_sort |
X chromosome inactivation during grasshopper spermatogenesis |
| dc.creator.none.fl_str_mv |
Viera Vicario, Alberto Parra Catalán, María Teresa Arévalo, Sara García de la Vega, Carlos Santos, Juan Luis Page Utrilla, Jesús |
| author |
Viera Vicario, Alberto |
| author_facet |
Viera Vicario, Alberto Parra Catalán, María Teresa Arévalo, Sara García de la Vega, Carlos Santos, Juan Luis Page Utrilla, Jesús |
| author_role |
author |
| author2 |
Parra Catalán, María Teresa Arévalo, Sara García de la Vega, Carlos Santos, Juan Luis Page Utrilla, Jesús |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Biología Facultad de Ciencias |
| dc.subject.none.fl_str_mv |
Animals Chromosome Pairing Epigenesis, Genetic Female Gene Silencing Grasshoppers Lysine Male Meiosis Meiotic Prophase I RNA Polymerase II Spermatocytes Spermatogenesis X Chromosome X Chromosome Inactivation Y Chromosome Biología y Biomedicina / Biología |
| topic |
Animals Chromosome Pairing Epigenesis, Genetic Female Gene Silencing Grasshoppers Lysine Male Meiosis Meiotic Prophase I RNA Polymerase II Spermatocytes Spermatogenesis X Chromosome X Chromosome Inactivation Y Chromosome Biología y Biomedicina / Biología |
| description |
Regulation of transcriptional activity during meiosis depends on the interrelated processes of recombination and synapsis. In eutherian mammal spermatocytes, transcription levels change during prophase-I, being low at the onset of meiosis but highly increased from pachytene up to the end of diplotene. However, X and Y chromosomes, which usually present unsynapsed regions throughout prophase-I in male meiosis, undergo a specific pattern of transcriptional inactivation. The interdependence of synapsis and transcription has mainly been studied in mammals, basically in mouse, but our knowledge in other unrelated phylogenetically species is more limited. To gain new insights on this issue, here we analyzed the relationship between synapsis and transcription in spermatocytes of the grasshopper Eyprepocnemis plorans. Autosomal chromosomes of this species achieve complete synapsis; however, the single X sex chromosome remains always unsynapsed and behaves as a univalent. We studied transcription in meiosis by immunolabeling with RNA polymerase II phosphorylated at serine 2 and found that whereas autosomes are active from leptotene up to diakinesis, the X chromosome is inactive throughout meiosis. This inactivation is accompanied by the accumulation of, at least, two repressive epigenetic modifications: H3 methylated at lysine 9 and H2AX phosphorylated at serine 139. Furthermore, we identified that X chromosome inactivation occurs in premeiotic spermatogonia. Overall, our results indicate: (i) transcription regulation in E. plorans spermatogenesis differs from the canonical pattern found in mammals and (ii) X chromosome inactivation is likely preceded by a process of heterochromatinization before the initiation of meiosis |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021-11-23 |
| dc.type.none.fl_str_mv |
research article http://purl.org/coar/resource_type/c_2df8fbb1 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10486/704712 https://dx.doi.org/10.3390/genes12121844 |
| url |
http://hdl.handle.net/10486/704712 https://dx.doi.org/10.3390/genes12121844 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI |
| publisher.none.fl_str_mv |
MDPI |
| dc.source.none.fl_str_mv |
reponame:Biblos-e Archivo. Repositorio Institucional de la UAM instname:Universidad Autónoma de Madrid |
| instname_str |
Universidad Autónoma de Madrid |
| reponame_str |
Biblos-e Archivo. Repositorio Institucional de la UAM |
| collection |
Biblos-e Archivo. Repositorio Institucional de la UAM |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869408417993457664 |
| score |
15.300719 |