Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction

Mango (Mangifera indica L.) peel and pulp are a source of dietary fiber (DF) and phenolic compounds (PCs) that constituent part of the indigestible fraction (IF). This fraction reaches the colon and acts as a carbon and energy source for intestinal microbiota. The effect of mango IF on intestinal mi...

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Autores: Gutiérrez Sarmiento, Wilbert, Sáyago Ayerdi, Sonia Guadalupe, Goñi Cambrodón, Isabel, Gutiérrez Miceli, Federico Antonio, Abud-Archila, Miguel, Rejón Orantes, José del Carmen, Rincón Rosales, Reiner, Peña Ocaña, Betsy Anaid, Ruíz Valdiviezo, Víctor Manuel
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/8651
Acceso en línea:https://hdl.handle.net/20.500.14352/8651
Access Level:acceso abierto
Palabra clave:612.39
: “Ataulfo” mango
in vitro colonic fermentation
intestinal microbiota
16S rRNA gene
bioinformatics
Dietética y nutrición (Farmacia)
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spelling Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible FractionGutiérrez Sarmiento, WilbertSáyago Ayerdi, Sonia GuadalupeGoñi Cambrodón, IsabelGutiérrez Miceli, Federico AntonioAbud-Archila, MiguelRejón Orantes, José del CarmenRincón Rosales, ReinerPeña Ocaña, Betsy AnaidRuíz Valdiviezo, Víctor Manuel612.39: “Ataulfo” mangoin vitro colonic fermentationintestinal microbiota16S rRNA genebioinformaticsDietética y nutrición (Farmacia)Mango (Mangifera indica L.) peel and pulp are a source of dietary fiber (DF) and phenolic compounds (PCs) that constituent part of the indigestible fraction (IF). This fraction reaches the colon and acts as a carbon and energy source for intestinal microbiota. The effect of mango IF on intestinal microbiota during colonic fermentation is unknown. In this study, the isolated IF of a novel ‘Ataulfo’ mango-based bar (snack) UV-C irradiated and non irradiated (UVMangoBand MangoB) were fermented. Colonic fermentation occurred in vitro under chemical-enzymatic, semi-anaerobic, batch culture and controlled pH colonic conditions. Changes in the structure of fecal microbiota were analyzed by 16s rRNA gene Illumina MiSeq sequencing. The community´s functional capabilities were determined in silico. The MangoB and UVMangoB increased the presence of Faecalibacterium, Roseburia, Eubacterium, Fusicatenibacter, Holdemanella, Catenibacterium, Phascolarctobacterium, Buttiauxella, Bifidobacterium, Collinsella, Prevotella and Bacteroides genera. The alpha indexes showed a decrease in microbial diversity after 6 h of colonic fermentation. The coordinates analysis indicated any differences between irradiated and non-irradiated bar. The metabolic prediction demonstrated that MangoB and UVMangoB increase the microbiota carbohydrate metabolism pathway. This study suggests that IF of mango-based bar induced beneficial changes on microbial ecology and metabolic pathway that could be promissory to prevention or treatment of metabolic dysbiosis. However, in vivo interventions are necessary to confirm the interactions between microbiota modulating and intestinal beneficial effects.MDPIUniversidad Complutense de Madrid20202020-03-0320202020-03-03journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/8651reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/86512026-06-02T12:44:21Z
dc.title.none.fl_str_mv Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction
title Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction
spellingShingle Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction
Gutiérrez Sarmiento, Wilbert
612.39
: “Ataulfo” mango
in vitro colonic fermentation
intestinal microbiota
16S rRNA gene
bioinformatics
Dietética y nutrición (Farmacia)
title_short Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction
title_full Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction
title_fullStr Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction
title_full_unstemmed Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction
title_sort Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction
dc.creator.none.fl_str_mv Gutiérrez Sarmiento, Wilbert
Sáyago Ayerdi, Sonia Guadalupe
Goñi Cambrodón, Isabel
Gutiérrez Miceli, Federico Antonio
Abud-Archila, Miguel
Rejón Orantes, José del Carmen
Rincón Rosales, Reiner
Peña Ocaña, Betsy Anaid
Ruíz Valdiviezo, Víctor Manuel
author Gutiérrez Sarmiento, Wilbert
author_facet Gutiérrez Sarmiento, Wilbert
Sáyago Ayerdi, Sonia Guadalupe
Goñi Cambrodón, Isabel
Gutiérrez Miceli, Federico Antonio
Abud-Archila, Miguel
Rejón Orantes, José del Carmen
Rincón Rosales, Reiner
Peña Ocaña, Betsy Anaid
Ruíz Valdiviezo, Víctor Manuel
author_role author
author2 Sáyago Ayerdi, Sonia Guadalupe
Goñi Cambrodón, Isabel
Gutiérrez Miceli, Federico Antonio
Abud-Archila, Miguel
Rejón Orantes, José del Carmen
Rincón Rosales, Reiner
Peña Ocaña, Betsy Anaid
Ruíz Valdiviezo, Víctor Manuel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 612.39
: “Ataulfo” mango
in vitro colonic fermentation
intestinal microbiota
16S rRNA gene
bioinformatics
Dietética y nutrición (Farmacia)
topic 612.39
: “Ataulfo” mango
in vitro colonic fermentation
intestinal microbiota
16S rRNA gene
bioinformatics
Dietética y nutrición (Farmacia)
description Mango (Mangifera indica L.) peel and pulp are a source of dietary fiber (DF) and phenolic compounds (PCs) that constituent part of the indigestible fraction (IF). This fraction reaches the colon and acts as a carbon and energy source for intestinal microbiota. The effect of mango IF on intestinal microbiota during colonic fermentation is unknown. In this study, the isolated IF of a novel ‘Ataulfo’ mango-based bar (snack) UV-C irradiated and non irradiated (UVMangoBand MangoB) were fermented. Colonic fermentation occurred in vitro under chemical-enzymatic, semi-anaerobic, batch culture and controlled pH colonic conditions. Changes in the structure of fecal microbiota were analyzed by 16s rRNA gene Illumina MiSeq sequencing. The community´s functional capabilities were determined in silico. The MangoB and UVMangoB increased the presence of Faecalibacterium, Roseburia, Eubacterium, Fusicatenibacter, Holdemanella, Catenibacterium, Phascolarctobacterium, Buttiauxella, Bifidobacterium, Collinsella, Prevotella and Bacteroides genera. The alpha indexes showed a decrease in microbial diversity after 6 h of colonic fermentation. The coordinates analysis indicated any differences between irradiated and non-irradiated bar. The metabolic prediction demonstrated that MangoB and UVMangoB increase the microbiota carbohydrate metabolism pathway. This study suggests that IF of mango-based bar induced beneficial changes on microbial ecology and metabolic pathway that could be promissory to prevention or treatment of metabolic dysbiosis. However, in vivo interventions are necessary to confirm the interactions between microbiota modulating and intestinal beneficial effects.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-03-03
2020
2020-03-03
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/8651
url https://hdl.handle.net/20.500.14352/8651
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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